PNA-DNA Hybridization Detection with One-Step Electrodeposition using Nanomaterial Modified Gold Electrode

Abstract

One of the important parameter that plays an important role in the performance of an electrochemical DNA biosensor is the supporting material for the immobilized probes. Using nanomaterials for electrode modification has gained a lot of attention with many advantages, such as large surface area and good electrical conductivity that can improve sensor sensitivity. The development of a direct PNA-DNA hybridization detection based on a novel pyrrolidinyl peptide nucleic acid or acpcPNA probe was studied using differential pulse voltammetric detections. Enhancement the sensitivity of DNA hybridization biosensor is presented using nanomaterial modified gold electrode. The oxidation current of an anthraquinone (AQ) tagged acpcPNA probe (acpcPNA-AQ) was measured from the electron transfer between the tagged AQ and the gold electrode based on differential pulse voltammetry (DPV).   From various gold modified electrodes, polyaniline-Graphene-Ag nanocomposite coated electrode surface provided   a higher sensitivity than polyaniline, polyaniline-Graphene and polyaniline-Ag due to its high surface area and good electrical conductivity of graphene and Ag nanoparticles. This DNA sensor can be used up to 33 times with 0.05 M of sodium hydroxide as the regeneration solution. Moreover, only one-step of electrodeposition of nanomaterial modification is very simple, cost-effective and fast detection of a specific DNA screening technique.