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Stachytarpheta jamaicensis (L.) Vahl is a traditional herb found across tropical regions, including Thailand. The aerial part of this plant was extracted with solvents : hexane, dichloromethane, methanol, and water, respectively. Then, crude extracts were tested antioxidant activities using DPPH, ABTS•+, and FRAP assay. The research results showed that methanol crude extract had the highest antioxidant activities whereas, dichloromethane crude extract had the highest total flavonoid content (61.93 mg CE/g extract). Therefore, it was further used for cytotoxicity test against human oral squamous carcinoma cell lines (CLS-354/WT and CLS-354/DX) and cell viability using MTT assay. It also found that CH2Cl2 crude extract exhibited a potent cytotoxicity against both cancer cell lines, and the IC50 concentrations of CH2Cl2 crude extract in the CLS-354/WT and CLS-354/DX were 197.7 ± 38.6 and 143.4 ± 21.2 µg/mL. This research results confirmed that the biological activity of phytochemicals from Stachytarpheta jamaicensis (L.) Vahl will probably be useful for the research and development of anti-oral cancer agents in the future.
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