Effect of 7,8-dihydro-8α-20-hydroxyecdysone on Photosynthesis, Chlorophyll Content and Chloroplast Structure in Tomato (Solanum lycopersicum cv. “CH154”) under Drought Stress
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Abstract
This research aimed to investigate the role of 7,8-Dihydro-8α-20-Hydroxyecdysone (DHECD) on drought tolerant ability, photosynthesis, chlorophyll content and chloroplast structure under drought by using Polyethylene Glycol 6000 (PEG) in tomato. The experiment 1 studied drought tolerant ability in tomato and measured shoot length, root length, Relative Water Content (RWC), chlorophyll and carotenoids contents. There were 4 treatments: 0, 0.25, 0.5, and 1 % (w/v) PEG whereas the experiment 2 determined the effect of DHECD on chlorophyll a/b ratio, performance index (PI), chlorophyll fluorescence (Fv/Fm), SPAD values and chloroplast ultrastructure in tomato under drought. There were 3 treatments: 1) control plants (0 %(w/v) PEG) and sprayed with 0 μM DHECD, 2) drought treated plants (0.5 % (w/v) PEG) and sprayed with 0 μM DHECD, and 3) drought treated plants (0.5 %(w/v) PEG) and sprayed with 50 μM DHECD. Experiment 1 results showed that shoot and root lengths, RWC, chlorophyll a, and chlorophyll b contents of drought treated plants (0.5 %(w/v) PEG) were decreased by 33.93 %, 60.57 %, 34.22 %, 12.47 % and 35.68 %, respectively when compared with control while carotenoids content increased by 47.20% when compared with control. Experiment 2 results showed that after 12 days of drought treatment, DHECD maintained chloroplast structure in leaves and increased chlorophyll a/b ratio, PI, Fv/Fm and SPAD values by 6.51 %, 21.22 %, 0.72 % and 25.43 %, respectively when compared with drought. These results indicated that DHECD application improves photosynthetic efficiency and maintains chlorophyll content and chloroplast structure in tomato under drought.
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References
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