The Enhancing Oil Pollution Remediation Sites with Effective Emulsifying Property of Biosurfactant–Producing Bacillus oceanisediminis PM 08


  • Chanika Saenge Chooklin Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, Trang, 92150, Thailand
  • Kattinat Sagulsawasdipan Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, Trang, 92150, Thailand
  • Aneak Sawain Faculty of Science and Fisheries Technology, Rajamangala University of Technology Srivijaya, Trang, 92150, Thailand
  • Atipan Saimmai Faculty of Agricultural Technology, Phuket Rajabhat University, Phuket, 83000, Thailand
  • Natthaporn Rattanapan Faculty of Agricultural Technology, Phuket Rajabhat University, Phuket, 83000, Thailand;
  • Wannakorn Kitcha Faculty of Science and Technology, Bansomdejchaopraya Rajabhat University, Bangkok, 10600



Biosurfactant, emulsifying, oil pollution remediation, Bacillus oceanisediminis PM 08


Bacillus oceanisediminis PM 08, isolated from soil and seawater in southern Thailand, was evaluated as a potential biosurfactant with effective emulsifying properties. This strain produced a biosurfactant with a maximum emulsifying activity of 65% and a surface tension of 22.67 mN/m after 60 hours of cultivation under cultivation conditions. The yield of biosurfactant obtained by extraction with chloroform: methanol (2: 1, vol/vol) was 1.55 g/L. The stability of the biosurfactant was effective over a wide range of pH, temperature, and salinity. The high thermostability of the biosurfactant was determined using a thermal simultaneous. In addition, a preliminary chemical composition using FT-IR revealed that it is a glycolipid biosurfactant. The properties of this biosurfactant make it a good product for many industrial applications under extreme conditions.


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