Effect of Chlorine Dioxide on Micropropagation of Gymnocalycium mihanovichii LB2178 Agua Dulce (Cactaceae)
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Abstract
Gymnocalycium mihanovichii LB2178 Agua Dulce is a succulent species popular in many areas. It has been cultivated for a long time. It is considered one of the easiest cacti to grow in Thailand. Therefore, the objective of this study was 1) to study the effect of Chlorine dioxide (ClO2) on culture medium sterilization on micropropagation of G. mihanovichii LB2178 Agua Dulce. and 2) to study the effect of Chlorine dioxide on shoot and root induction of G. mihanovichii LB2178 Agua Dulce. The G. mihanovichii LB2178 Agua Dulce seeds were used as plant material. After 2 months of culture, the results showed that MS medium supplemented with 50 ppm ClO2 gave the highest survival rate at 54.17%. After 3 months of culture, the results showed that shoot and root induction can be achieved in G. mihanovichii LB2178 Agua Dulce seeds were cultured on MS medium supplemented with 50 ppm ClO2 gave the highest number of roots at 9.00 roots/explant, length of root 3.45 cm, length of shoot 0.54 cm and diameter of shoot 0.44 cm.
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