Pharmacognostic Investigation, Optimization of Extraction Condition and Determination of Piperine in Black Pepper Fruit

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Published: Jun 30, 2024
DOI: https://doi.org/10.55164/ajstr.v27i4.253186
Keywords:
Pharmacognostic investigation optimization black pepper fruit

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Lukman Sueree
Faculty of Science and Technology, Hatyai University, Hatyai, Songkhla 90110, Thailand
Chaowalit Monton
Drug and Herbal Product Research and Development Center, College of Pharmacy, Rangsit University, Pathum Thani, 12000, Thailand
Aurawan Boonyataeng
Faculty of Science and Technology, Hatyai University, Hatyai, Songkhla, 90110, Thailand
Auttapong Pinthongpan
Faculty of Science and Technology, Hatyai University, Hatyai, Songkhla, 90110, Thailand

Abstract

This study aimed to investigate the standardization parameters through qualitative analyses of black pepper fruit. Microscopic characteristics of the crude drug were demonstrated. The study determined various parameters including loss on drying, total ash, acid-insoluble ash, water-soluble extractives, ethanol-soluble extractives, and volatile oil content. Additionally, piperine content in ethanol extract was evaluated using Thin Layer Chromatography (TLC) fingerprint analysis and High-Performance Liquid Chromatography (HPLC) analysis. The optimal conditions for piperine extraction were determined through experimental design. The study provides pharmacognostic specifications towards the fundamental standardization of Piper nigrum Linn. fruit.

Article Details

Issue
Vol. 27 No. 4 (2024): July - August
Section
Research Articles
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This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.

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