Metabolite Profiling and Morphological Screening of C. militaris Fruiting Bodies Extracts using UHPLC-QTOF-IMS and GC-MS Analysis
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Abstract
The medicinal mushroom C. militaris has several health advantages and has been utilized for many years throughout Asia as a component of traditional medicine systems. It can be used as a functional food and in nutraceutical products. This study investigated the morphological characteristics of C. militaris during the large-scale cultivation and metabolic profiling of the ethanolic and aqueous extracts of their fruiting bodies. The cultural and morphological characteristics of C. militaris were studied during the growth of this mushroom in terms of production of mycelial growth and fruiting bodies by conventional microbiological techniques. Cordycepin content in the aqueous and ethanol extracts of fruiting bodies was evaluated using UHPLC-QTOF-IMS analysis. The detection of metabolites in the ethanol extract was done by GC-MS analysis. The cordycepin content in the ethanol and aqueous extracts of the fruiting bodies was found to be 16.92 mg/g and 10.88 mg/g, respectively. GC-MS spectra analysis of the C. militaris fruiting bodies ethanolic extracts indicated the existence of eighteen metabolites such as 3,4-Dihydroxymandelic acid-terms, n-Hexadecanoic acid, Ethyl pentadecanoate, 1, E-11, Z-13-Octadecatriene, 9,12-Octadecadienoic acid (Z, Z)-, I-9-Octadecenoic acid ethyl ester, 9,12-Octadecadienoic acid (Z, Z)-, Trimethyls, 9(11)-Dehydroergosterol tosylate, Ergosterol, Silane, (phenyloxiranylidene) bis[trimethy, Neophytadiene, 1-Octadecyne, n-Hexadecanoic acid, Ethyl 9-hexadecenoate, 2,5-Diiodo-9-oxabicyclo [4.2.1] nonane, i-Propyl 9,12,15-octadecatrienoate, Ergosta-4,7,22-trien-3.beta.-ol, and TMS Palmitic acid. Evaluating cordycepin content and other bio components of C. militaris will help exploit this mushroom for potential medicinal benefits and develop reasonable quality pharmaceutical, nutraceutical, and functional food products.
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