Effect of Different Luteinizing Hormone Releasing Hormone Analogs (LHRHa) on Spawning in Mahseer barb (Neolissochilus stracheyi)
Article Sidebar
Main Article Content
Abstract
The study assessed the impact of varying doses of luteinizing hormone releasing hormone analogs (LHRHa) on key reproductive parameters in Mahseer barb fish (N. stracheyi). Four treatment groups were established. Trt1 (control) received 0.9% sodium chloride of 0.3 mL/kg BW with 10 mg domperidone (Dom)/kg BW; Trt2 was administered 20 μg/kg BW of LHRHa with 10 mg Dom/kg BW; Trt3 received 25 μg/kg BW of LHRHa with 10 mg Dom/kg BW; and Trt4 was given 30 μg/kg BW of LHRHa with 10 mg Dom/kg BW. Each treatment was replicated three times with three fish per replicate. The outcomes revealed a 100% success rate in ovulation induction across all groups using LHRHa with Dom. Trt4, with the highest dosage, achieved the most notable results, showing an ovulation index of 95.12 ± 2.54%. In contrast, the control group (Trt1) recorded the lowest ovulation index at 6.10 ± 0.73%. The application of 30 μg/kg BW of LHRHa with 10 mg Dom/kg BW in Trt4 resulted in the quickest latency period of 30.35 ± 0.60 hours and significantly improved the fertilization rate compared to the lower dosages used in Trt2 and Trt3. However, Trt4 not only demonstrated the highest efficacy in terms of latency, number of ovulated eggs, fertilization rate, hatching rate, and survival rate but also confirmed the superior potential of the highest LHRHa dosage of 30 μg/kg BW combined with Dom for successful spawning induction in Mahseer barb fish.
Article Details
This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Day, F. Monograph of Indian Cyprinidae. Parts 1–3. J. Proc. Asiatic Soc. Bengal. 1871, 40(2), 95–142. https://zoobank.org/References/3F4C7611-9627-4632-A5E8-CAA7C9D874E1.
Pinder, A. C.; Raghavan, R. Conserving the Endangered Mahseers (Tor spp.) of India: The Positive Role of Recreational Fisheries. Curr. Sci. 2013, 104(11), 1472–1475. https://www.researchgate.net/publication/237067345.
Pinder, A. C.; Britton, R.; Harrison, A. J.; Raghavan, R.; et al. Mahseer (Tor spp.) Fishes of the World: Status, Challenges and Opportunities for Conservation. Rev. Fish Biol. Fish. 2019, 29(2–3), 417–452. https://doi.org/10.1007/s11160-019-09566-y.
Abass, Z.; Shah, T. H.; Bhat, F. A.; Ramteke, K.; Magloo, A. H.; Hamid, I.; Wanjari, R. N.; Somasundharam, I. The Mahseer: The Tiger of Water—An Angler's Delight in the Himalayas and the Undisputed King of Sport Fishing. Fish. Res. 2024, 279, 107147. https://doi.org/10.1016/j.fishres.2024.107147.
Surachat, K.; Deachamag, P.; Wonglapsuwan, M. The First De Novo Genome Assembly and Sex Marker Identification of Pluang Chomphu Fish (Tor tambra) from Southern Thailand. Comput. Struct. Biotechnol. J. 2022, 20, 1470–1480. https://doi.org/10.1016/j.csbj.2022.03.021.
Jaapar, M. Z.; Norhana, W. N. M. H.; Yusof, H. M.; Hamzah, A.; et al. Research and Innovation in Malaysian Mahseer (Tor sp.) Broodstock Development Programme. Asian Fish. Sci. 2023, 36(4), 219–232. https://doi.org/10.33997/j.afs.2023.36.4.004.
Triyanti, R.; Suryawati, S. H.; Wijaya, R. A.; Hafsaridewi, R.; et al. Assessment of the Success Factors Influencing Rice-Fish Farming Innovation Village to Support Food Security. IOP Conf. Ser. Earth Environ. Sci. 2021, 892(1), 012052. https://doi.org/10.1088/1755-1315/892/1/012052.
Das, S. C. S.; Pathak, R. K.; Khan, A. U.; Sarkar, U.; et al. Pattern of Reproductive Biology of the Endangered Golden Mahseer (Tor putitora, Hamilton 1822) with Special Reference to Regional Climate Change Implications on Breeding Phenology. J. Appl. Anim. Res. 2018, 46(1), 864–872. https://doi.org/10.1080/09712119.2018.1497493.
Shahi, N.; Pandey, J.; Mallik, S.; Das, P.; et al. Gonadal Development Stages of Wild Male Golden Mahseer (Tor putitora) from Nainital Region of Uttarakhand, India. J. Ecophysiol. Occup. Health 2015, 14(3–4), 133. https://doi.org/10.15512/joeoh/2014/v14i3-4/59998.
Sharma, A.; Sarma, D.; Joshi, R.; Das, P.; Akhtar, M. S.; Pande, V.; Sharma, P. Gonad Indices, Morphology, and Muscle Fatty Acid Compositions of Male and Female Golden Mahseer (Tor putitora) Sampled from Lake Bhimtal (Himalaya) at Different Seasons of the Year. Aquac. Fish. 2024, 9(4), 603–616. https://doi.org/10.1016/j.aaf.2022.08.002.
Dash, P.; Tandel, R. S.; Bhat, R. A. H.; Sarma, D.; Pandey, N.; Sawant, P. B.; Chadha, N. K. Spawning Substrate Preference and Spawning Behavior of Chocolate Mahseer (Neolissochilus hexagonolepis). Anim. Reprod. Sci. 2021, 233, 106847. https://doi.org/10.1016/j.anireprosci.2021.106847.
Duangjai, E.; Punya-in, S.; A-ta, A.; Arin, S.; Sangkamruang, A.; Sresutham, P.; Punroob, J. Effects of Omega-3 Fatty Acids (from Krill Oil) on Sperm Quality of Mahseer Barb, Neolissochilus stracheyi, Brooders Reared in Captivity. SNRU J. Sci. Technol. 2017, 9(2), 474–482. https://thaiscience.info/Journals/Article/SNRU/10997775.pdf
Ismail, M. F. S.; Siraj, S. S.; Daud, S. K.; Harmin, S. A. Association of Annual Hormonal Profile with Gonad Maturity of Mahseer (Tor tambroides) in Captivity. Gen. Comp. Endocrinol. 2011, 170(1), 125–130. https://doi.org/10.1016/j.ygcen.2010.09.021.
El-Hawarry, W. N.; Abd El-Rahman, S. H.; Shourbela, R. M. Breeding Response and Larval Quality of African Catfish (Clarias gariepinus, Burchell 1822) Using Different Hormones/Hormonal Analogues with Dopamine Antagonist. Egypt. J. Aquat. Res. 2016, 42(2), 231–239. https://doi.org/10.1016/j.ejar.2016.06.003.
Islam, P.; Hossain, M. I.; Khatun, P.; Masud, R. I.; Tasnim, S.; Anjum, M.; Islam, M. Z.; Nibir, S. S.; Rafiq, K.; Islam, M. A. Steroid Hormones in Fish, Caution for Present and Future: A Review. Toxicol. Rep. 2024, 13, 101733. https://doi.org/10.1016/j.toxrep.2024.101733.
Mylonas, C. C.; Fostier, A.; Zanuy, S. Broodstock Management and Hormonal Manipulations of Fish Reproduction. Gen. Comp. Endocrinol. 2010, 165(3), 516–534. https://doi.org/10.1016/j.ygcen.2009.03.007.
Roy, K. Rapid Review on the Use of New Age Induced Breeding Agent LHRHa in Indian Finfish Seed Production Sector. J. Fish. 2016, 4 (2), 401–407.https://doi.org/10.17017/jfish.v4i2.2016.145.
Nithirojpakdee, P.; Jaisuk, C.; Keereelang, J.; Kongsuk, M. Effects of Luteinizing Hormone-Releasing Hormone Analogue (LHRHa) on Ovulation and Spawning of Schistura kohchangensis. Int. J. Agric. Technol. 2024, 20(2), 619–630. http://www.ijat-aatsea.com.
Washim, M. R.; Ahmmed, S.; Rube, A. K. M. The Effects of Synthetic Gonadotropin Releasing Hormone Analogue (SGnRHa) on Artificial Propagation of Spotted Scat (Scatophagus argus). Asian J. Anim. Sci. 2022, 16(2), 123–132. https://file-khulna.portal.gov.bd.
Fermin, A. C. LHRH-a and Domperidone-Induced Oocyte Maturation and Ovulation in Bighead Carp, Aristichthys nobilis (Richardson). Aquaculture 1991, 93(1), 87–94. https://doi.org/10.1016/0044-8486(91)90207-N.
Harmin, S. A.; Crim, L. W. Gonadotropic Hormone-Releasing Hormone Analog (GnRH-A) Induced Ovulation and Spawning in Female Winter Flounder, Pseudopleuronectes americanus (Walbaum). Aquaculture 1992, 104 (3–4), 375–390. https://doi.org/10.1016/0044-8486(92)90218-A.
Tan-Fermin, J. D.; Pagador, R. R.; Chavez, R. C. LHRHa and Pimozide-Induced Spawning of Asian Catfish Clarias macrocephalus (Gunther) at Different Times During an Annual Reproductive Cycle. Aquaculture 1997, 148(4), 323–331. https://doi.org/10.1016/S0044-8486(96)01423-8.
Cahyanti, W.; Soelistyowati, D. T.; Carman, O.; Kristanto, A. H. Artificial Spawning and Larvae Performance of Three Indonesian Mahseer Species. AACL Bioflux 2019, 12(1), 1–7. http://www.bioflux.com.ro/aacl.
Pirhonen, J.; Schreck, C. B. Effects of Anaesthesia with MS-222, Clove Oil, and CO2 on Feed Intake and Plasma Cortisol in Steelhead Trout (Oncorhynchus mykiss). Aquaculture 2003, 220(1–4), 507–514. https://doi.org/10.1016/S0044-8486(02)00624-5.
Mylonas, C. C.; Zohar, Y. Use of GnRHa-Delivery Systems for the Control of Reproduction in Fish. Rev. Fish Biol. Fish. 2000, 10(4), 463–491. https://doi.org/10.1023/A:1012266030077.
Mylonas, C. C.; Fostier, A.; Zanuy, S. Broodstock Management and Hormonal Manipulation of Fish Reproduction. Gen. Comp. Endocrinol. 2009, 165(3), 516–534. https://doi.org/10.1016/j.ygcen.2009.03.007.
Nwokoye, C. O.; Nwuba, L. A.; Eyo, J. Induced Propagation of African Clariid Catfish, Heterobranchus bidorsalis (Geoffrey Saint Hillarie, 1809) Using Synthetic and Homoplastic Hormones. Afr. J. Biotechnol. 2007, 6(23). https://doi.org/10.5897/AJB2007.000-2430.
Trachtenberg, J. The Effect of the Chronic Administration of a Potent Luteinizing Hormone Releasing Hormone Analog on the Rat Prostate. J. Urol. 1982, 128(5), 1097–1100. https://doi.org/10.1016/s0022-5347(17)53352-2.
Sreenivasulu, G.; Senthilkumaran, B.; Sridevi, P.; Rajakumar, A.; Rasheeda, M. K. Expression and Immunolocalization of 20β-Hydroxysteroid Dehydrogenase during Testicular Cycle and after hCG Induction, in vivo in the Catfish, Clarias gariepinus. Gen. Comp. Endocrinol. 2012, 175(1), 48–54. https://doi.org/10.1016/j.ygcen.2011.09.002.
Maradun, H. F.; Ahmad, M.; Sahabi, A. M.; Umar, F.; Ibrahim, J. Z.; Adamu, I. Effect of Different Male-Female Broodstock Ratios on the Induced Breeding Performance of Clarias gariepinus. Int. J. Adv. Res. 2018, 6(1), 388–393. https://doi.org/10.21474/IJAR01/6278.
Aruho, C.; Nattabi, J. K.; Masembe, C.; Nyeko, J.; Rutaisire, J. Assessment of Artificial Breeding and Hatchery Management Practices of Labeobarbus altianalis in Uganda. Aquacult. Res. 2020, 51(6), 2384–2394. https://doi.org/10.1111/are.14602.
