Germination-Mediated Alterations in Physicochemical, Functional and Cooking Properties of Vigna aconitifolia Flour
Main Article Content
Abstract
A Vigna aconitifolia (V.aconitifolia), also known as moth bean, is a nutrient-rich legume that is commonly consumed in many parts of the world. Germination is a process that can enhance the nutritional and functional properties of legumes. However, there is limited information on the effects of germination on the physicochemical, functional, and cooking attributes of V. aconitifolia. The objectives of this study were to evaluate the effects of germination on the physicochemical, functional, and cooking attributes of V. aconitifolia and to compare the properties of germinated and ungerminated V. aconitifolia seeds. Seeds were germinated for 24 hours and then dried and milled into flour. The physico-chemical properties of the flour, including moisture content, ash content, and pH, were evaluated. The functional properties, including water absorption, oil absorption, and emulsification capacities, were also evaluated. The cooking characteristics, including cooking time, water uptake ratio, and swelling power, were evaluated using standard methods. The results showed that germination significantly improved the physicochemical properties of V.aconitifolia, including moisture content, ash content, and pH. The functional properties, including water absorption, oil absorption, and emulsification capacities, were also significantly improved. The cooking characteristics, including cooking time, water uptake ratio and swelling power, were significantly reduced. The results suggest that germination can enhance the nutritional and functional properties of V.aconitifolia, but may also affect its cooking characteristics. The findings of this study have significant implications for the food industry, as they suggest that germination can be a simple and effective method to enhance the quality and functionality of legume flours.
Article Details

This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License.
References
Kumar, D.; Singh, M.; Kushwaha, M. Nutrient content, uptake and NUE of oats (2). Indian Journal of Agronomy 2021, 66, 466-473.
National Nutrition Monitoring Board Report. Government of India. 2019.
Singh, R.; Kølvraa, S.; Bross, P.; Christensen, K.; Bathum, L.; Gregersen, N.; Tan, Q.; Rattan, S.I.S. Anti-inflammatory heat shock protein 70 genes are positively associated with human survival, Current Pharmaceutical Design 2010, 16(7), 796-801. https://doi.org/10.2174/138161210790883499
Tomooka, N.; Senthil, N.; Pandiyan, M.; Ramamoorthi, N.; Kaga, A.; Vaughan, D.A. Collection and conservation of leguminous crops and their wild relatives in Tamil Nadu, India, Annual Report on Exploration and Introduction of Plant Genetic Resources (NIAS, Tsukuba, Japan) 2008, 24, 113-125.
Tomooka, N.; Pandiyan, M.; Senthil, N.; Ramamoorthi, N.; Kaga, A.; Vaughan, D.A. Collection and conservation of leguminous crops and their wild relatives in Tamil Nadu, India, Annual Report on Exploration and Introduction of Plant Genetic Resources (NIAS,Tsukuba, Japan) 2009, 25, 83-109.
Kumar, R.; Ghoshal, G.; Goyal, M. Biodegradable composite films/coatings of modified corn starch/gelatin for shelf life improvement of cucumber. J Food Sci Technol. 2021, 58(4), 1227-1237. https://doi.org/10.1007/s13197-020-04685-y
Venipriyadharshini, L.; Kavitha, K. Moth Bean (Vigna aconitifolia) as Potential Supplement to Evaluate the Weight Gain in Wistar Albino Rats (Rattus norvegicus). International Journal of Experimental Research and Review 2023, 36, 127-134. https://doi.org/10.52756/ijerr.2023.v36.012
González-Fonteboa, F.; Martínez-Abella, R.; Rodríguez-Álvaro, E.; Rey-Bouzón, S.; Seara-Paz, M.F. Herrador,3 - Use of coal bottom ash and other waste as fine aggregates in lightweight cement-based materials, Woodhead Publishing 2021, 53-87. https://doi.org/10.1016/B978-0-12-820549-5.00026-7
Zare, F.; Orsat, V.; Boye, J. Functional, Physical and Sensory Properties of Pulse Ingredients Incorporated into Orange and Apple Juice Beverages, Journal of Food Research 2015, 4, 5. https://doi.org/10.5539/jfr.v4n5p143
Olika, E.; Abera, S.; Fikre, A. Physicochemical Properties and Effect of Processing Methods on Mineral Composition and Antinutritional Factors of Improved Chickpea (CicerarietinumL.) Varieties Grown in Ethiopia, International Journal of Food Science 2019, 1-7. https://doi.org/10.1155/2019/9614570
Kumar, R.; Ghoshal, G.; Goyal, M. Moth bean starch (Vignaaconitifolia): isolation, characterization, and development of edible/biodegradable films, Journal of Food Science and Technology 2019, 56(11), 4891-4900. https://doi.org/10.1007/s13197-019-03959-4
Azeredo, HMC.; Waldron, KW. Crosslinking in polysaccharide and protein films and coatings for food contact–a review, Trends Food Science Technology 2016, 52, 109-122. https://doi.org/10.1016/j.tifs.2016.04.008
Mohsenin, N.N. Physical properties of plant and animal materials. Vol. 1. Structure, physical characterisitics and mechanical properties 1970, 1, 734.
Shreelalitha, S.J.; Sridhar, K.R. Physical and Cooking Properties of Seeds of Two Wild Legume Landraces of Sesbania, International Journal of Agricultural Technology 2018, 14(3), 363-376.
Ghasemi-Varnamkhasti, M.; Mobli, H.; Jafari, A.; Keyhani, A.R.; Hedori Soltanabadi, M.; Rafiee, S.; Kheiralipour, K. Some physical properties of rough rice (Oryza Sativa L.) grain. Journal of Cereal Science 2008, 47, 496-501. https://doi.org/10.1016/j.jcs.2007.05.014
Mijivoievic, M.; Zita, R.; Tania, P. Izmene ISTA rules changes in seed germination testing at the beginning of the 21st century, Journal on Processing and Energy in Agriculture 2018, 22(2), 40-45 https://doi.org/10.5937/JPEA1801040M
Surender, M.; Sagare, D.; Setti, P. Durga, Ch.V.; FarzanaJabeen, Rani.; Sudarshan, M.R.; Sokka Reddy, S. Mean Performance of Normal and QPM Maize Genotypes forYield and Tryptophan Content, International Journal of Current Microbiology and Applied Sciences 2017, 6(11), 830-844. https://doi.org/10.20546/ijcmas.2017.611.098
American Society of Agricultural Engineers. ASAE S352.2: Moisture Measurement-Unground grain and seeds. In ASAE Standards 2001, 549-555.
Matouk. Physical properties of legumes. Journal of Food Science and Technology 2018, 55(2), 533-539.
Tosh, S.M.; Yada, S. Dietary fibres in pulse seeds and fractions: characterization, functional attributes and applications. Food Research International 2010, 43, 450-460. https://doi.org/10.1016/j.foodres.2009.09.005
Flores, F.; Singh, J.; Kerr, W.L.; Parrish, D.; Shi, Y. Physical and chemical properties of sweet potato purees during processing. Journal of Food Science 2012, 77(4), S1448-S1455. https://doi.org/10.1111/j.1750-3841.2012.02672.x
Evelyn, S.; Buckman, Ibok Oduro,Wisdom A. Plahar,Charles Tortoe, Determination of the chemical and functional properties of yam bean (Pachyrhizuserosus (L.) Urban) flour for food systems, Food Science & Nutrition 2017, 6(2), 457-463. https://doi.org/10.1002/fsn3.574
Shuang-kui, D.; Hongxin, J.; Xiuzhu,Y.; Jay-lin, J. Physicochemical and functional properties of whole legume flour, LWT-Food Science and Technology 2014, 55(1), 308-313. https://doi.org/10.1016/j.lwt.2013.06.001
Salma, H. A.; Nahid, A. A.; ElShazali, A. M.; Isam, Elfadil E. B. Changes in the functional properties as a function of NaCl concentration of legumes protein isolate by Trans glutaminase cross linking International Food Research Journal 2010, 17, 817-824.
Adeleke, R.O.; Odedeji, J.O. Functional Properties of Wheat and Sweet Potato Flour Blends. Pakistan Journal of Nutrition 2010, 9, 535-538. http://dx.doi.org/10.3923/pjn.2010.535.538.
Ghumman, A.; Kaur, A.; Singh, N. Functionality and digestibility of albumins and globulins from lentil and horse gram and their effect on starch rheology, Food Hydrocoll 2016, 61, 843-850. https://doi.org/10.1016/j.foodhyd.2016.07.013
Wani, IA.; Sogi, DS. Hamdani. Isolation, composition, and physicochemical properties of starch from legumes: a review. Starch/Starke 2016, 68, 834-845. https://doi.org/10.1002/star.201600007
Zhang, Y.; Li, M.; Wang, X. Anthocyanins regulate seedling growth and development by modulating auxin signaling. Plant Physiology 2019, 181(2), 641-653. https://doi.org/10.1104/pp.19.00851
Wang, X.; Li, Z.; Chen, G. Chlorophyll biosynthesis and photosynthesis in germinating rice seeds. Journal of Plant Physiology, 2020, 247, 153141. https://doi.org/10.1016/j.jplph.2020.153141
Balakrishnan, P.; Sreekala, MS., Thomas, S. Morphology, transport characteristics and viscoelastic polymer chain confinement in nano composites based on thermoplastic potato starch and cellulose nano fibers from pineapple leaf. CarbohydrPolym 2017, 169, 176-188. https://doi.org/10.1016/j.carbpol.2017.04.017
Chandrasekara, A.; Shahidi, F. Determination of antioxidant activity in free and hydrolyzed fractions of millet grains and characterization of their phenolic profiles by HPLC-DAD-ESI-MSⁿ. Journal of Functional Foods 2011, 3(3), 144-158. https://doi.org/10.1016/j.jff.2011.03.006
Nkhata, S. G.; Ayua, E.; Kamau, E. H.; Shingiro, J. B. Fermentation and germination improve nutritional value and functionality of foods: A review. Food Science & Nutrition 2018, 6(8), 2449-2458. https://doi.org/10.1002/fsn3.846