Biodegradation of Oil and Grease in Synthetic Wastewater Using Lipase-Producing Bacteria Isolated from University Canteen Wastewater
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Abstract
This study aims to isolate lipase-producing bacteria from university canteen wastewater and assess their effectiveness in the degradation of oil and grease in synthetic wastewater. The results showed that the bacteria isolated from the grease trap wastewater of the central canteen exhibited a bacterial count of 9.3 × 108 CFU/mL. In total, 11 bacterial isolates, labeled A1 to A11, were obtained, displaying six distinct colony morphologies. Lipase-producing bacteria were selected based on the size of the clear zone formed, with isolates A6 and A11 being selected for further evaluation. The effectiveness of these isolates in degrading oil and grease in synthetic wastewater was then assessed. The optimal initial concentration of oil and grease for bacterial degradation was found to be 10,480 mg/L at 32 °C over 40 hours. Bacteria A6 and A11 demonstrated average degradation efficiencies of 24.52% and 18.14%, respectively. Therefore, this study serves as a preliminary laboratory investigation aimed at generating essential data for the potential future applications in treating wastewater contaminated with oil and grease.
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