Effect of Plant Growth Regulators on In Vitro Shoot and Root Induction and Acclimatization of Gymnocalycium mihanovichii LB2178 Agua Dulce (Cactaceae)
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Abstract
Gymnocalycium mihanovichii LB2178 Agua Dulce is a popular cactus still in demand, known for its distinctive shape and ease of growth. It can be propagated by tissue culture to produce strong, disease-free plants. Therefore, the aim of this research was 1) to study the effect of BA and IBA hormones and types of explants on the growth rate of G. mihanovichii LB2178 Agua Dulce cactus. and 2) to study suitable planting materials for survival and growth of G. mihanovichii LB2178 Agua Dulce cactus under natural conditions. After 3 months of culture, the results showed that the explant sectioned longitudinally were cultured on MS medium supplemented with 0 and 2 mg/L BA gave the highest survival, 100%, and shoot induction, 86.67%, the explant sectioned transversally were cultured on MS medium supplemented with 1 mg/L BA gave the highest number of shoot, 4.20 shoots/explant, and shoot length, 0.36 mm respectively, significant difference at p≤0.05. Following three months of cultivation, the findings indicated that shoot explants cultured on MS medium supplemented with 1 mg/L IBA showed the highest root induction (91.67%), average number of roots (20.47 roots/explant), and average root length (7.63 mm), with significant differences at p≤0.05. After 3 months, the rooted plants were removed from the tissue culture room and transferred to sterile plastic pots. The result showed that G. mihanovichii LB2178 Agua Dulce grown in potting soil: sand, showed the highest survival rate of 100%.
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