The combination of different carriers in producing plant-based seasoning powder from oyster mushroom (Pleurotus sajor-caju)

Main Article Content

Nguyen Thi Ngoc Giang
Khai Tran Van
Ho Thi Ngan Ha

Abstract

The increasing popularity and prioritization of plant-based nutritional foods have heightened the focus on research and the diversification of plant-derived products. Therefore, this paper centers on producing a plant-based seasoning powder from oyster mushroom, an available commodity, using its concentrated extract and various carriers to maximize retention of bioactive nutrients and ensure compliance with the product's physicochemical and microbiological quality standards. The optimal ratio of the combination between maltodextrin (MD) and gum arabic (GA) was obtained through a range of investigated ratios, which was a completely randomized setup with concentrations of 5, 10, 15, and 20% for MD, and 0, 0.5, 1.0, 1.5, and 2% for GA.  The outcome has shown that MD combined with GA significantly affected the seasoning powder product; at a mixing ratio of 10% MD and 1% GA, it exhibited the highest stability and optimal physicochemical properties. The high retention of key bioactive compounds, including flavonoids, phenolics, lysine, glutamic acid, and β-glucan, with respective contents of 0.0034 g QE, 0.28 g TAE, 0.0052 mg, 2.32 mg, and 10.86 mg per 100 g dry matter. The powder demonstrated satisfactory yield and solubility, and the brightness index was the highest among all tested formulations. Moreover, the water activity reached 0.42, ensuring the product's enzymatic and microbiological stability.

Article Details

Section
Research Articles

References

Giang, N. T. N.; Khai, T. V.; Thuy, N. M. Changes in yield and quality of Pleurotus sajor-caju by seasons, harvest times and during maturation. Vietnam J. Agric. Rural Dev. 2021, 1, 39–47.

Galappaththi, M. C. A.; Dauner, L.; Madawala, S.; Karunarathna, S. C. Nutritional and medicinal benefits of oyster (Pleurotus) mushrooms: a review. Fungal Biotec. 2021, 1(2), 65–87. https://doi.org/10.5943/FunBiotec/1/2/5

Fogarasi, M.; et al. Bioactive secondary metabolites in mushrooms: A focus on polyphenols, their health benefits and applications. Food Biosci. 2024, 62, 105166. https://doi.org/10.1016/j.fbio.2024.105166

Subramaniam, S.; Jiao, S.; Zhang, Z.; Jing, P. Impact of post-harvest processing or thermal dehydration on physiochemical, nutritional and sensory quality of shiitake mushrooms. Compr. Rev. Food Sci. Food Saf. 2021, 20(3), 2560–2595. https://doi.org/10.1111/1541-4337.12738

Giang, N. T. N.; Thuy, N. M. Effects of drying methods on the characteristics of Pleurotus sajor-caju mushroom. Malays. Appl. Biol. 2020, 49(3), 31–36. https://doi.org/10.55230/mabjournal.v49i3.1538

Giang, N. T. N.; Khai, T. V.; Thuy, N. M. The influence of packaging and storage temperature on the chemical composition of fresh oyster mushrooms (Pleurotus sajor-caju) during storage. Acta Sci. Pol. Technol. Aliment. 2022, 21(3), 261–269. https://doi.org/10.17306/J.AFS.2022.1007

Nolden, A. A.; Forde, C. G. The nutritional quality of plant-based foods. Sustainability 2023, 15(4), 3324. https://doi.org/10.3390/su15043324

Xue, J.; Yin, Y. Plant-based food: From nutritional value to health benefits. Foods 2024, 13(22), 3595. https://doi.org/10.3390/foods13223595

Medawar, E.; Huhn, S.; Villringer, A.; Witte, A. V. The effects of plant-based diets on the body and the brain: a systematic review. Transl. Psychiatry 2019, 9(1), 226. https://doi.org/10.1038/s41398-019-0552-0

Jiménez-González, O.; Guerrero-Beltrán, J. Á. Extraction, microencapsulation, color properties, and experimental design of natural pigments obtained by spray drying. Food Eng. Rev. 2021, 13(4), 769–811. https://doi.org/10.1007/s12393-021-09288-7

Pudžiuvelytė, L.; Petrauskaitė, E.; Stabrauskienė, J.; Bernatonienė, J. Spray-drying microencapsulation of natural bioactives: Advances in sustainable wall materials. Pharmaceuticals 2025, 18(7), 963. https://doi.org/10.3390/ph18070963

Kandasamy, S.; Naveen, R. A review on the encapsulation of bioactive components using spray‐drying and freeze‐drying techniques. J. Food Process Eng. 2022, 45(8), e14059. https://doi.org/10.1111/jfpe.14059

Ribeiro, A. M.; Shahgol, M.; Estevinho, B. N.; Rocha, F. Microencapsulation of vitamin A by spraydrying, using binary and ternary blends of gum arabic, starch and maltodextrin. Food Hydrocoll. 2020, 108, 106029. https://doi.org/10.1016/j.foodhyd.2020.106029

Sukri, N.; Multisona, R. R.; Zaida; Saputra, R. A.; Mahani; Nurhadi, B. Effect of maltodextrin and arabic gum ratio on physicochemical characteristic of spray dried propolis microcapsules. Int. J. Food Eng. 2021, 17(2), 159–165. https://doi.org/10.1515/ijfe-2019-0050

Bednarska, M. A.; Janiszewska-Turak, E. The influence of spray drying parameters and carrier material on the physico-chemical properties and quality of chokeberry juice powder. J. Food Sci. Technol. 2020, 57 (2), 564–577. https://doi.org/10.1007/s13197-019-04088-8

Thanh, D. V.; Ha, H. T. N.; Tan, N. D.; Giang, N. T. N. Combining carrier materials for spray-drying antioxidant extracts from Palmyra palm peel (Borassus flabellifer): optimization using RSM and ANN-GA. Acta Sci. Pol. Technol. Aliment. 2025, 24(3), 427–440.

Giang, N. T. N. Study on the production of concentrated extracts and their application in the production of mushroom-based sauce from oyster mushrooms (Pleurotus spp.). Ph.D. Dissertation, Can Tho University, Can Tho, Vietnam, 2023 (In Vietnamese).

Cano-Chauca, M.; Stringheta, P. C.; Ramos, A. M.; Cal-Vidal, J. Effect of the carriers on the microstructure of mango powder obtained by spray drying and its functional characterization. Innov. Food Sci. Emerg. Technol. 2005, 6(4), 420–428. https://doi.org/10.1016/j.ifset.2005.05.003

Stauß, A. C.; Fuchs, C.; Jansen, P.; Repert, S.; Alcock, K.; Ludewig, S.; Rozhon, W. The ninhydrin reaction revisited: Optimisation and application for quantification of free amino acids. Molecules 2024, 29(14), 3262. https://doi.org/10.3390/molecules29143262

Hasani, M.; Yaghoubi, L.; Abdollahi, H. A kinetic spectrophotometric method for simultaneous determination of glycine and lysine by artificial neural networks. Anal. Biochem. 2007, 365(1), 74–81. https://doi.org/10.1016/j.ab.2007.02.010

Fournier, E. Colorimetric quantification of carbohydrates. Curr. Protoc. Food Anal. Chem. 2001, 1(1), E1.1.1–E1.1.8. https://doi.org/10.1002/0471142913.fae0101s00

Sumaiyah; Masfria; Dalimunthe, A. Determination of total phenolic content, total flavonoid content, and antimutagenic activity of ethanol extract nanoparticles of Raphidophora pinnata (L.F) schott. Leaves. Rasayan J. Chem. 2018, 11(2), 505–510. https://doi.org/10.31788/RJC.2018.1122068

Sarabandi, K.; et al. Effect of different carriers on microstructure and physical characteristics of spray dried apple juice concentrate. J. Food Sci. Technol. 2018, 55(8), 3098–3109. https://doi.org/10.1007/s13197-018-3235-6

Shishir, M. R.; Chen, W. Trends of spray drying: A critical review on drying of fruit and vegetable juices. Trends Food Sci. Technol. 2017, 65, 49–67. https://doi.org/10.1016/j.tifs.2017.05.002

Bazaria, B.; Kumar, P. Effect of whey protein concentrate as drying aid and drying parameters on physicochemical and functional properties of spray dried beetroot juice concentrate. Food Biosci. 2016, 14, 21–27. https://doi.org/10.1016/j.fbio.2015.11.002

Escobedo-Avellaneda, Z.; Rodríguez-Martínez, V.; Serment-Moreno, V.; Velázquez, G.; Welti-Chanes, J.; Torres, J. A. Selected applications of water activity management in the food industry. In Water Activity in Foods: Fundamentals and Applications, 2nd ed.; Barbosa-Cánovas, G. V., Fontana, A. J., Schmidt, S. J., Labuza, T. P., Eds.; Wiley: Hoboken, NJ, USA, 2020; pp 465–482

Valková, V.; et al. Impact of freeze- and spray-drying microencapsulation techniques on β-glucan powder biological activity: A comparative study. Foods 2022, 11(15), 2267. https://doi.org/10.3390/foods11152267

Michalska, A.; Lech, K. The Effect of carrier quantity and drying method on the physical properties of apple juice powders. Beverages 2018, 4(1), 2. https://doi.org/10.3390/beverages4010002

Cao, X.; Zhang, M.; Qian, H.; Mujumdar, A. S.; Wang, Z. Physicochemical and nutraceutical properties of barley grass powder microencapsulated by spray drying. Dry. Technol. 2017, 35(11), 1358–1367. DOI: 10.1080/07373937.2017.1332074

Barbosa-Cánovas, G. V.; Fontana, A. J., Jr.; Schmidt, S. J.; Labuza, T. P. Water Activity in Foods: Fundamentals and Applications, 2nd ed.; John Wiley & Sons: Hoboken, NJ, 2020.

Nurhidajah; Boby, P.; Muhammad, Y.; Yunan, K. S.; Diode, Y. Microencapsulation of Umami Flavor Enhancer from Indonesian Waters Brown Seaweed. Curr. Res. Nutr. Food Sci. 2022, 10(1), 349–359. https://doi.org/10.12944/CRNFSJ.10.1.29

Sharma, A.; Jana, A. H.; Chavan, R. S. Functionality of milk powders and milk-based powders for end use applications: a review. Compr. Rev. Food Sci. Food Saf. 2012, 11(5), 518–528. https://doi.org/10.1111/j.1541-4337.2012.00199.x

Lee, J. K. M.; Taip, F. S.; Abdullah, Z. Effectiveness of additives in spray drying performance: a review. Food Res. 2018, 2(6), 486–499. https://doi.org/10.26656/fr.2017.2(6).134

Toikkanen, O.; Outinen, M.; Malafrante, L.; Rojan, O. J. Formation and structure of insoluble particles in reconstituted model infant formula powders. Int. Dairy J. 2018, 82, 19–27. https://doi.org/10.1016/j.idairyj.2018.03.001

Etzbach, L.; Meinert, M.; Faber, T.; Klein, C.; Schieber, A.; Weber, F. Effects of carrier agents on powder properties, stability of carotenoids, and encapsulation efficiency of goldenberry (Physalis peruviana L.) powder produced by co-current spray drying. Curr. Res. Food Sci. 2020, 3, 73–81. https://doi.org/10.1016/j.crfs.2020.03.002

Chavan, G. M.; et al. Enhancing functional and physical properties of spray dried mixed fruit juices using composite carrier agents. Ital. J. Food Sci. 2025, 37(4), 34–46. https://doi.org/10.15586/ijfs.v37i4.3122

Ho, T. M.; Chan, S.; Yago, A. J. E.; Shravya, R.; Bhandari, B. R.; Bansal, N. Changes in physicochemical properties of spray-dried camel milk powder over accelerated storage. Food Chem. 2019, 295, 224–233. https://doi.org/10.1016/j.foodchem.2019.05.122

Bhusari, S. N.; Muzaffar, K.; Kumar, P. Effect of carrier agents on physical and microstructural properties of spray dried tamarind pulp powder. Powder Technol. 2014, 266, 354–364. https://doi.org/10.1016/j.powtec.2014.06.038

Nordin, N. L.; Bakar, J.; Adzahan, N. M.; Razis, A. F. A.; Ismail, N.; Sulaiman, R. Microencapsulation of bioactive volatile compounds from MD2 pineapple peel Extract using spray-drying and foam-mat drying. J. Agric. Food Res. 2024, 18, 101539. https://doi.org/10.1016/j.jafr.2024.101539

Thuy, N. M.; Hao, H. V.; Duong, L. T. T.; Giau, T. N.; Minh, V. Q.; Tai, N. V. Foam-mat drying of lucuma powder: Mathematical and artificial modeling of drying kinetics, physicochemical and microstructural properties. J. Agric. Food Res. 2025, 19, 101656. https://doi.org/10.1016/j.jafr.2025.101656

Laureanti, E. J. G.; Paiva, T. S.; de Matos-Jorge, L. M.; Jorge, R. M. M. Microencapsulation of bioactive compound extracts using maltodextrin and gum arabic by spray and freeze-drying techniques. Int. J. Biol. Macromol. 2023, 253, 126969. https://doi.org/10.1016/j.ijbiomac.2023.126969

Parhizkary, M.; Hasanpour, R.; Assadpour, E.; Jafari, S. M. Spray-drying encapsulation of jujube extract: Enhancing bioactivity and stability via maltodextrin-based carriers with polysaccharides and proteins. Carbohydr. Polym. Technol. Appl. 2025, 10, 100869. https://doi.org/10.1016/j.carpta.2025.100869

Xu, Y.; et al. The application of encapsulation technology in the food Industry: Classifications, recent advances, and perspectives. Food Chem. X 2024, 21, 101240. https://doi.org/10.1016/j.fochx.2024.101240

Iesa, N. B.; et al. Effects of maltodextrin and gum arabic composition on the physical and antioxidant activities of dewaxed stingless bee cerumen. Foods 2023, 12(20), 3740. https://doi.org/10.3390/foods12203740

Gomes, W. F.; et al. Effect of freeze- and spray-drying on physico-chemical characteristics, phenolic compounds and antioxidant activity of papaya pulp. J. Food Sci. Technol. 2018, 55 (6), 2095–2102. https://doi.org/10.1007/s13197-018-3124-z

Rahman, M. M. A.; Ranganathan, T. V. Encapsulation of isoflavone with milk, maltodextrin and gum acacia improves its stability. Curr. Res. Food Sci. 2020, 2, 77–83. https://doi.org/10.1016/j.crfs.2019.12.003

Kurek, M. A.; Pratap-Singh, A. Plant-based (hemp, pea and rice) protein-maltodextrin combinations as wall material for spray-drying microencapsulation of hempseed (Cannabis sativa) oil. Foods 2020, 9 (11), 1707. https://doi.org/10.3390/foods9111707

Pérez-Pérez, V.; Jiménez-Martínez, C.; González-Escobar, J. L.; Corzo-Ríos, L. J. Exploring the impact of encapsulation on the stability and bioactivity of peptides extracted from botanical sources: trends and opportunities. Front. Chem. 2024, 12, 1423500. https://doi.org/10.3389/fchem.2024.1423500

Rao, P. S.; Bajaj, R. K.; Mann, B.; Arora, S.; Tomar, S. K. Encapsulation of antioxidant peptide enriched casein hydrolysate using maltodextrin-gum arabic blend. J. Food Sci. Technol. 2016, 53(10), 3834–3843. https://doi.org/10.1007/s13197-016-2376-8

Buljeta, I.; Pichler, A.; Šimunović, J.; Kopjar, M. Polysaccharides as carriers of polyphenols: comparison of freeze-drying and spray-drying as encapsulation techniques. Molecules 2022, 27(16), 5069. https://doi.org/10.3390/molecules27165069

Rezvankhah, A.; Emam-Djomeh, Z.; Safari, M.; Salami, M.; Askari, G. Investigating the effects of maltodextrin, gum arabic, and whey protein concentrate on the microencapsulation efficiency and oxidation stability of hemp seed oil. J. Food Process. Preserv. 2022, 46(6), e16554. https://doi.org/10.1111/jfpp.16554

Li, R. Y.; Shi, Y. Microencapsulation of borage oil with blends of milk protein, β-glucan and maltodextrin through spray drying: physicochemical characteristics and stability of the microcapsules. J. Sci. Food Agric. 2018, 98(3), 896–904. https://doi.org/10.1002/jsfa.8535

Giang, N. T. N.; Tan, N. D.; Ha, H. T. N.; Thanh, D. V.; Diem, L. T. T.; Khai, T. V.; Quyen, D. K. Sustainable and novel approach for valorizing nutritional component in oyster mushrooms (Pleurotus sajor-caju) hydrolysates: An optimization study by ANN-GA. J. Agric. Food Res. 2025, 24, 102351. https://doi.org/10.1016/j.jafr.2025.102351

Giang, N. T. N.; Tan, N. D.; Ha, H. T. N.; Thanh, D. V.; Diem, L. T. T.; Khai, T. V.; Diem, M. L.; et al. Artificial intelligence prediction the desirable moisture content of dried oyster mushroom (Pleurotus sajor-caju) for enhancing the cellulase-assisted extraction efficiency. J. Agric. Food Res. 2025, 19, 101561. https://doi.org/10.1016/j.jafr.2024.101561

Gayathry, K. S.; John, J. A. Physical, functional and bioactive properties of microencapsulated powders from banana pseudostem and inflorescence extracts. Food Prod. Process and Nutr. 2024, 6(1), 74. https://doi.org/10.1186/s43014-024-00251-7

Fournaise, T.; Petit, J.; Gaiani, C. Main powder physicochemical characteristics influencing their reconstitution behavior. Powder Technol. 2021, 383, 65–73. https://doi.org/10.1016/j.powtec.2021.01.056