Dietary Lecithin Modulates Growth, Selected Hemato-immunological Indices, and Glutathione Peroxidase Activity of Hoven's Carp (Leptobarbus hoevenii)
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Abstract
Beneficial effects of dietary phospholipids on growth performance, hemato-immunological parameters, and antioxidant status in Hoven's carp (Leptobarbus hoevenii) are reported in this study. Experimental fish were fed four isoproteic (40%) and isolipidic (13%) experimental diets supplemented with lecithin at 0, 1, 3, and 5% for 90 days. The results showed that at 30, 60, and 90 days into the feeding trial, fish fed dietary supplementation containing 3% or 5% lecithin showed significantly improved total length, mean body weight, feed conversion ratio, and specific growth rate compared to the control group (p<0.05). No significant differences in condition factor and survival rate were observed between fish receiving dietary lecithin at any tested concentration and the control group (p>0.05). A study on hemato-immunological parameters of fish fed the experimental diets for 90 days revealed the highest red blood cell count in those receiving the 1% lecithin diet (p<0.05), whereas the white blood cell count was significantly higher in fish fed 3% and 5% lecithin diets (p<0.05). In addition, fish fed a 3% lecithin diet showed significantly higher glutathione peroxidase activity than those fed with the control diet or the 1% lecithin diet (p<0.05). These findings suggest that dietary supplementation with lecithin modulates growth and some hematological and immunological parameters, as well as antioxidant activity, in this fish species. Based on the findings, dietary supplementation with lecithin at 3-5% could promote growth, stimulate the immune response, and enhance antioxidant activity in Hoven's carp.
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References
Srithongthum, S.; Au, H. L.; Amornsakun, T.; Musikarun, P.; Fatihah, S. N.; Halid, N. F. A.; Lim, L. S. Observation on the Embryonic Development of Sultan Fish, Leptobarbus hoevenii. AACL Bioflux 2021, 14(3), 1359–1364.
Kwanmuang, S.; Kraisurasi, S.; Phetrit, N.; Jiamton, W. Ah... Is There Fish Too? Pla Baa. Thai Fish. Gaz. 2016, 69(1), 77–79.
Au, H. L.; Lim, L. S.; Amornsakun, T.; Rahmah, S.; Liew, H. J.; Musikarun, P.; Promkaew, P.; Mok, W. J.; Kawamura, G.; Yong, A. S.; Shapawi, R.; Mustafa, S. Feeding and Nutrients Requirement of Sultan Fish, Leptobarbus hoevenii: A Review. Int. J. Aqu. Sci. 2020, 11(1), 3–12.
Sunarno, M. T. D. Growth and Nutrient Digestibility of Jelawat (Leptobarbus hoevenii) Fry Fed with Various Dietary Protein Levels. Indones. Fish. Res. J. 2002, 8(1), 19–25. https://doi.org/10.15578/ifrj.8.1.2002.19-25.
Farahiyah, I. J.; Zainal Abidin, A. R.; Ahmad, A.; Wong, H. K. Optimum Protein Requirement for the Growth of Jelawat Fish (Leptobarbus hoevenii). Mal. J. Anim. Sci. 2017, 20(2), 39–46.
Tocher, D. R.; Bendiksen, E. Å.; Campbell, P. J.; Bell, J. G. The Role of Phospholipids in Nutrition and Metabolism of Teleost Fish. Aquaculture 2008, 280(1–4), 21–34. https://doi.org/10.1016/j.aquaculture.2008.04.034
Ukwela, E. J.; Muhammad, S. R. S.; Mazelan, S.; Mohamad, S. J.; Chian, W. C.; Vethamony, P.; Al-Khayat, J.; Rosas, V. T.; Jung, L. H. Benefits of Phospholipids in Aquafeed Development: A Review. Planet. Sust. 2024, 2(1), 5–24. https://doi.org/10.46754/ps.2024.01.002
Wee, W.; Téllez-Isaías, G.; Abdul Kari, Z.; Cheadoloh, R.; Kabir, M. A.; Mat, K.; Mohamad Sukri, S. A.; Rahman, M. M.; Rusli, N. D.; Wei, L. S. The Roles of Soybean Lecithin in Aquafeed: A Crucial Need and Update. Front. Vet. Sci. 2023, 10, 1188659. https://doi.org/10.3389/fvets.2023.1188659
Balito-Liboon, J. S.; Ferdinand, R.; Traifalgar, M.; Pagapulan, M. J. B. B.; Mameloco, E. J. G.; Temario, E. E.; Corre, V. L., Jr. Dietary Soybean Lecithin Enhances Growth Performance, Feed Utilization Efficiency and Body Composition of Early Juvenile Milkfish, Chanos chanos. Isr. J. Aquacult.-Bamid. 2018, 70. https://doi.org/10.46989/001c.20927
Amer, A.-R.; Eweedah, N. M.; Amer, A. A.; Gewaily, M. S.; Younis, N. A.; Ahmed, H. A.; Dawood, M. A. O. Dietary Effect of Soybean Lecithin on the Growth Performance, Digestive Enzyme Activity, Blood Biomarkers, and Antioxidative Status of Striped Catfish, Pangasianodon hypophthalmus. PLoS One 2023, 18(10), e0291954. https://doi.org/10.1371/journal.pone.0291954
Wu, J.; Yang, W.; Song, R.; Li, Z.; Jia, X.; Zhang, H.; Zhang, P.; Xue, X.; Li, S.; Xie, Y.; Zhang, R.; Ye, J.; Zhou, Z.; Wu, C. Dietary Soybean Lecithin Improves Growth, Immunity, Antioxidant Capability and Intestinal Barrier Functions in Largemouth Bass Micropterus salmoides Juveniles. Metabolites 2023, 13(4), 512. https://doi.org/10.3390/metabo13040512
AOAC. Official Methods of Analysis of AOAC International, 18th ed.; AOAC International: Gaithersburg, MD, USA, 2005.
Boyd, C. E.; Tucker, C. S. Water Quality and Pond Soil Analyses for Aquaculture; Alabama Agricultural Experiment Station, Auburn University: Alabama, USA, 1992.
Upasit, D.; Chiayvareesajja, J.; Chiayvareesajja, S. Reproductive Biology of Johnius carouna (Cuvier, 1830) off the Coast of Songkhla Province. Thaksin Univ J. 2017, 20(3), 43–50.
Kliangtapong, A.; Thongprajukaew, K.; Rodjaroen, S. Growth Performance of Oranda Goldfish (Carassius auratus) Fed Diets Containing Cyanobacteria Hapalosiphon welwitschii TISTR 8237. RMUTSV Res. J. 2018, 10(3), 356–367.
Kamnerddee, C.; Puangpee, S.; Kaewkong, P.; Nuntapong, N.; Wanlem, S.; Suanyuk, N. Dietary Supplementation with Zooshikella marina Improves Growth Performance, Haemato-Immunological Parameters and Disease Resistance against Streptococcus agalactiae in Nile Tilapia (Oreochromis niloticus). Songklanakarin J. Sci. Technol. 2024, 46(2), 83–91.
Mylonas, C. C.; Cardinaletti, G.; Sigelaki, I.; Polzonetti-Magni, A. Comparative Efficacy of Clove Oil and 2-Phenoxyethanol as Anesthetics in the Aquaculture of European Sea Bass (Dicentrarchus labrax) and Gilthead Sea Bream (Sparus aurata) at Different Temperatures. Aquaculture 2005, 246(1–4), 467–481. https://doi.org/10.1016/j.aquaculture.2005.02.046
Suwannasang, A.; Dangwetngam, M.; Issaro, A.; Phromkunthong, W.; Suanyuk, N. Pathological Manifestations and Immune Responses of Serotypes Ia and III Streptococcus agalactiae Infections in Nile Tilapia (Oreochromis niloticus). Songklanakarin J. Sci. Technol. 2014, 36(5), 499–506.
Malawa, S.; Nuntapong, N.; Suanyuk, N.; Thongprajukaew, K. Addition of Different Concentrations of Indian Almond (Terminalia catappa) Leaf Extract to Aquarium Water Resulted in Improved Water Quality and Increased Bubble Nest Formation by Male Siamese Fighting Fish (Betta splendens) without Having Any Consistent Negative Effects on Growth Metrics and Blood Chemistry. Aquacult. Int. 2022, 30, 3269–3288. https://doi.org/10.1007/s10499-022-00960-1
Lowry, O. H.; Rosebrough, N. J.; Farr, A. L.; Randall, R. J. Protein Measurement with the Folin-Phenol Reagent. J. Biol. Chem. 1951, 193(1), 265–275. https://doi.org/10.1016/S0021-9258(19)52451-6
Stasiak, S. A.; Baumann, P. C. Neutrophil Activity as a Potential Bioindicator for Contaminant Analysis. Fish Shellfish Immunol. 1996, 6(7), 537–539. https://doi.org/10.1006/fsim.1996.0050
Demers, N. E.; Bayne, C. J. The Immediate Effects of Stress on Hormones and Plasma Lysozyme in Rainbow Trout. Dev. Comp. Immunol. 1997, 21(4), 363–373. https://doi.org/10.1016/S0145-305X(97)00009-8
Hyvärinen, A.; Nikkilä, E. A. Specific Determination of Blood Glucose with o-Toluidine. Clin. Chim. Acta 1962, 7(1), 140–143. https://doi.org/10.1016/0009-8981(62)90133-X.
Ávila-Villa, L. A.; Fimbres-Olivarria, D.; Garcia-Sánchez, G.; Gollas-Galván, T.; Hernández-López, J.; Martínez-Porchas, M. Physiological and Immune Responses of White Shrimp (Litopenaeus vannamei) Infected with Necrotizing Hepatopancreatitis Bacterium. Aquaculture 2012, 324–325, 14–19. https://doi.org/10.1016/j.aquaculture.2011.09.010
Trasviña-Arenas, C. H.; Garcia-Triana, A.; Peregrino-Uriarte, A. B.; Yepiz-Plascencia, G. White Shrimp Litopenaeus vannamei Catalase: Gene Structure, Expression and Activity under Hypoxia and Reoxygenation. Comp. Biochem. Physiol. B Biochem. Mol. Biol. 2013, 164, 44–52. https://doi.org/10.1016/j.cbpb.2012.10.004
Senphan, T.; Benjakul, S. Compositions and Yield of Lipids Extracted from Hepatopancreas of Pacific White Shrimp (Litopenaeus vannamei) as Affected by Prior Autolysis. Food Chem. 2012, 134(2), 829–835. https://doi.org/10.1016/j.foodchem.2012.02.188
Sink, T. D.; Lochmann, R. T. The Effects of Soybean Lecithin Supplementation to a Practical Diet Formulation on Juvenile Channel Catfish, Ictalurus punctatus: Growth, Survival, Hematology, Innate Immune Activity, and Lipid Biochemistry. J. World Aquac. Soc. 2014, 45(2), 163–172. https://doi.org/10.1111/jwas.12108
Jafari, F.; Agh, N.; Noori, F.; Tokmachi, A.; Gisbert, E. Effects of Dietary Soybean Lecithin on Growth Performance, Blood Chemistry and Immunity in Juvenile Stellate Sturgeon (Acipenser stellatus). Fish Shellfish Immunol. 2018, 80, 487–496. https://doi.org/10.1016/j.fsi.2018.06.023
Batista, R. O.; Richter, B. L.; Banze, J. F.; Schleder, D. D.; Salhi, M.; Nobrega, R. O.; Silva, M. F. O.; Mattioni, B.; Pettigrew, J. E.; Fracalossi, D. M. Soy Lecithin Supplementation Promotes Growth and Increases Lipid Digestibility in GIFT Nile Tilapia Raised at Suboptimal Temperature. Fishes 2023, 8(8), 404. https://doi.org/10.3390/fishes8080404
Yang, W.; Wu, J.; Song, R.; Li, Z.; Jia, X.; Qian, P.; Zhang, H.; Zhang, P.; Xue, X.; Li, S.; Xie, Y.; Ye, J.; Dong, G.; Wu, C. Effects of Dietary Soybean Lecithin on Growth Performance, Body Composition, Serum Biochemical Parameters, Digestive and Metabolic Abilities in Largemouth Bass Micropterus salmoides. Aquac. Rep. 2023, 29, 101528. https://doi.org/10.1016/j.aqrep.2023.101528
Robert, C.; Couëdelo, L.; Vaysse, C.; Michalski, M. C. Vegetable Lecithins: A Review of Their Compositional Diversity, Impact on Lipid Metabolism and Potential in Cardiometabolic Disease Prevention. Biochimie 2020, 169, 121–132. https://doi.org/10.1016/j.biochi.2019.11.017
Boyd, C. E. Water Quality for Pond Aquaculture; Research and Development Series No. 43; International Center for Aquaculture and Aquatic Environments, Alabama Agricultural Experiment Station, Auburn University: Alabama, USA, 1998.
Lucas, J. S.; Southgate, P. C.; Tucker, C. S. Aquaculture: Farming Aquatic Animals and Plants, 3rd ed.; John Wiley & Sons Ltd.: Chichester, UK, 2019.
Wu, Y.; Wang, T. Soybean Lecithin Fractionation and Functionality. J. Am. Oil Chem. Soc. 2003, 80, 319–326. https://doi.org/10.1007/s11746-003-0697-x
van Nieuwenhuyzen, W.; Tomás, M. C. Update on Vegetable Lecithin and Phospholipid Technologies. Eur. J. Lipid Sci. Technol. 2008, 110(5), 472–486. https://doi.org/10.1002/ejlt.200800041
Guiotto, E. N.; Tomás, M. C.; Diehl, B. W. K. Sunflower Lecithin. In Polar Lipids; Ahmad, M. U., Xu, X., Eds.; Elsevier: Amsterdam, The Netherlands, 2015; pp 57–75. https://doi.org/10.1016/B978-1-63067-044-3.50007-8
Ciji, A.; Akhtar, M. S.; Tripathi, P. H.; Pandey, A.; Rajesh, M.; Kamalam, B. S. Dietary Soy Lecithin Augments Antioxidative Defense and Thermal Tolerance but Fails to Modulate Non-Specific Immune Genes in Endangered Golden Mahseer (Tor putitora) Fry. Fish Shellfish Immunol. 2021, 109, 34–40. https://doi.org/10.1016/j.fsi.2020.11.031
Butler, G.; Candebat, C. L.; Das, S. K.; Nankervis, L. Improving Lipid Utilization and Growth through Lecithin Inclusion in Diets for Giant Grouper (Epinephelus lanceolatus). Anim. Feed Sci. Technol. 2025, 326, 116393. https://doi.org/10.1016/j.anifeedsci.2025.116393
Sivaramakrishnan, T.; Ambasankar, K.; Kumaraguru Vasagam, K. P.; Syama Dayal, J.; Sandeep, K. P.; Bera, A.; Makesh, M.; Kailasam, M.; Vijayan, K. K. Effect of Dietary Soy Lecithin Inclusion Levels on Growth, Feed Utilization, Fatty Acid Profile, Deformity and Survival of Milkfish (Chanos chanos) Larvae. Aquac. Res. 2021, 52, 5366–5374. https://doi.org/10.1111/are.15406
Ando, S.; Mori, Y.; Nakamura, K.; Sugawara, A. Characteristics of Lipid Accumulation Types in Five Species of Fish. Nippon Suisan Gakkaishi 1993, 59(9), 1559–1564. https://doi.org/10.2331/suisan.59.1559
Steffens, W.; Wirth, M. Freshwater Fish—An Important Source of n-3 Polyunsaturated Fatty Acids: A Review. Arch. Pol. Fish. 2005, 13(1), 5–16.
Talukdar, H.; Kalita, D.; Singh, K. B. Current Perspective on Omega-3 Fatty Acids in Freshwater Fish from India: A Review of Scientific Progress. Int. J. Zool. Appl. Biosci. 2025, 10(6), 173–180. https://doi.org/10.55126/ijzab.2025.v10.i06.019
Bahurmiz, O. M.; Ng, W. K. Effects of Dietary Palm Oil Source on Growth, Tissue Fatty Acid Composition and Nutrient Digestibility of Red Hybrid Tilapia, Oreochromis sp., Raised from Stocking to Marketable Size. Aquaculture 2007, 262(2–4), 382–392. https://doi.org/10.1016/j.aquaculture.2006.11.023
Khieokhajonkhet, A.; Klongchai, S.; Maphum, O.; Kaneko, G. Lipid Distribution Patterns of Nine Commercial Fish in Thailand. Aquac. Res. 2019, 50, 1348–1360. https://doi.org/10.1111/are.14011
Monir, M. S.; Yusoff, S. M.; Zulperi, Z. M.; Hassim, H. A.; Mohamad, A.; Ngoo, M. S. M. H.; Ina-Salwany, M. Y. Haemato-Immunological Responses and Effectiveness of Feed-Based Bivalent Vaccine against Streptococcus iniae and Aeromonas hydrophila Infections in Hybrid Red Tilapia (Oreochromis mossambicus × O. niloticus). BMC Vet. Res. 2020, 16, 226. https://doi.org/10.1186/s12917-020-02443-y
Esmaeili, M. Blood Performance: A New Formula for Fish Growth and Health. Biology 2021, 10(12), 1236. https://doi.org/10.3390/biology10121236
Torsabo, D.; Idris, N.; Iber, B. T.; Arome, A. G.; Chu, I. K. C.; Abduh, M. Y.; Noordin, N. M.; Azra, M. N.; Impellitteri, F.; Faggio, C.; Abdol-Munafi, A. B. Effects of Dietary Phospholipids on Growth, Biochemical Composition, Fatty Acid Profiles, Blood Metrics, and Sex Steroid Hormone of Male Pangasius nasutus Broodstock. Aquac. Stud. 2025, 25 (1), 47–63. https://doi.org/10.4194/AQUAST1935
Torsabo, D.; Iber, B. T.; Idris, N.; Okomoda, V. T.; Koh, I. C. C.; Abduh, M. Y.; Noordin, N. M.; Abdol-Munafi, A. B. Phospholipid-Supplemented Diet Impacts on Growth, Blood Metrics, Reproductive Indices, and Fatty Acid Profiles of Pangasianodon hypophthalmus. Aquac. Rep. 2023, 33, 101802. https://doi.org/10.1016/j.aqrep.2023.101802
Torsabo, D.; Iber, B. T.; Idris, N.; Arome, A. G.; Okomoda, V. T.; Chu, I. K. C.; Abduh, M. Y.; Noordin, N. M.; Abdol-Munafi, A. B. Dietary Phospholipid Supplementation Affects Blood Metrics, Reproductive Indices, and Biochemical Parameters of Female Shark Catfish, Pangasius nasutus. J. Fish. Environ. 2025, 49(1), 46–66.
Balfry, S. K.; Higgs, D. A. Influence of Dietary Lipid Composition on the Immune System and Disease Resistance of Finfish. In Nutritional and Fish Health; Lim, C., Webster, C. D., Eds.; The Haworth Press: New York, NY, USA, 2001; pp 213–234.
Pagheh, E.; Agh, N.; Marammazi, J. G.; Nouri, F.; Sepahdari, A.; Gisbert, E.; Torfi Mozanzadeh, M. Dietary Soybean Lecithin Affects Growth Performance, Fillet Biochemical Composition and Digestive Enzyme Activity in Sparidentex hasta Juveniles. J. Appl. Anim. Res. 2019, 47(1), 24–33. https://doi.org/10.1080/09712119.2018.1557663
Lubos, E.; Loscalzo, J.; Handy, D. E. Glutathione Peroxidase-1 in Health and Disease: From Molecular Mechanisms to Therapeutic Opportunities. Antioxid. Redox Signal. 2011, 15(7), 1957–1997. https://doi.org/10.1089/ars.2010.3586
Anwar, S.; Alrumaihi, F.; Sarwar, T.; Babiker, A. Y.; Khan, A. A.; Prabhu, S. V.; Rahmani, A. H. Exploring Therapeutic Potential of Catalase: Strategies in Disease Prevention and Management. Biomolecules 2024, 14, 697. https://doi.org/10.3390/biom14060697
Mourente, G.; Díaz-Salvago, E.; Bell, J. G.; Tocher, D. R. Increased Activities of Hepatic Antioxidant Defence Enzymes in Juvenile Gilthead Sea Bream (Sparus aurata L.) Fed Dietary Oxidised Oil: Attenuation by Dietary Vitamin E. Aquaculture 2002, 214(1–4), 343–361. https://doi.org/10.1016/S0044-8486(02)00064-9
Zhang, H.; Mu, Z.; Xu, L.; Xu, G.; Liu, M.; Shan, A. Dietary Lipid Level Induced Antioxidant Response in Manchurian Trout, Brachymystax lenok (Pallas) Larvae. Lipids 2009, 44, 643–654. https://doi.org/10.1007/s11745-009-3313-7
Zengin, H. The Effects of Feeding and Starvation on Antioxidant Defence, Fatty Acid Composition and Lipid Peroxidation in Reared Oncorhynchus mykiss Fry. Sci. Rep. 2021, 11, 16716. https://doi.org/10.1038/s41598-021-96204-y
Annamalay, S. D. Effects of Antioxidants on Oxidative Stress: Assessing MDA in Urine Samples. Int. J. Clin. Nutr. Diet. 2018, 4, 135. https://doi.org/10.15344/2456-8171/2018/135
