Effects of Vacuum and Modified Atmosphere Packaging on Physicochemical and Microbiological Quality of Cooked Squid Stuffed with Sticky Rice During Refrigerated Storage
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Abstract
Stuffed squid with sticky rice (Tupa Sutong) is a traditional seafood product from southern Thailand with limited refrigerated shelf life because of rapid microbial spoilage and quality deterioration. This study evaluated the effects of atmospheric packaging (AP), vacuum packaging (VP), and modified atmosphere packaging (MAP; 50% O₂, 25% CO₂, and 25% N₂) on the physicochemical and microbiological quality of cooked stuffed squid during refrigerated storage (4 ± 2 °C) for 24 days. Changes in hardness, pH, total volatile basic nitrogen (TVB-N), trimethylamine nitrogen (TMA-N), thiobarbituric acid (TBA), and total viable count (TVC) were determined throughout storage. Storage time significantly affected all quality parameters (p 0.05). Samples stored under AP deteriorated most rapidly, showing faster increases in TVB-N, TMA-N, TBA, and TVC, accompanied by a greater reduction in texture quality. Both VP and MAP delayed quality deterioration by slowing microbial growth and chemical changes during storage. Based on the microbiological acceptability limit, the shelf life of the product was 12, 18, and 21 days under AP, MAP, and VP, respectively. Among the packaging systems evaluated, VP provided the greatest extension of refrigerated shelf life while maintaining the physicochemical and microbiological quality of cooked stuffed squid with sticky rice. These findings provide useful information for selecting appropriate packaging systems for the commercial production and distribution of this traditional seafood product.
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References
[1] Kheokao, J.; Nopsuwan, R.; Phovijit, P.; Pornsuksawat, S.; Plianrung, S. Tracing the acculturation of food in Thailand's three southern border provinces. Asia-Pacific J. Compassion Stud. 2022, 7(1), 29–39. https://doi.org/10.13140/RG.2.2.31398.33600
[2] Plankensteiner, S. L.; Faxholm, P. L.; Olsen, K.; Mouritsen, O. G.; Frøst, M. B. Physicochemical characterization of sous vide cooked squid (Loligo forbesii and Loligo vulgaris) and the relationship to selected sensory properties and hedonic response. Int. J. Gastron. Food Sci. 2021, 23, 100298. https://doi.org/ 10.1016/j.ijgfs.2020.100298
[3] Manoj, D.; Auddy, I.; Nimbkar, S.; Chittibabu, S.; Shanmugasundaram, S. Development of co-immobilized enzymes amperometric biosensor for the determination of triglycerides in coconut milk. Int. Food Res. J. 2020, 27(5), 875–882.
[4] Gokoglu, N.; Ceylan, A. Improving the Physicochemical and Textural Properties of Squid (Loligo vulgaris) Muscle by Sous-Vide Cooking in Different Time-Temperature Combinations. J. Aquat. Food Prod. Technol. 2022, 31(8), 872–881. https://doi.org/10.1080/10498850.2022.2108360
[5] Moreira, L. B.; Antunes, R.; Cotas, J.; Martins, N.; Costa, N.; Noronha, J. P.; Mata, P.; Diniz, M. Modified Atmosphere Packaging (MAP) for Seaweed Conservation: Impact on Physicochemical Characteristics and Microbiological Activity. Foods 2023, 12(14), 2736. https://doi.org/10.3390/foods12142736
[6] Chen, X.; Zhao, J.; Zhu, L.; Luo, X.; Mao, Y.; Hopkins, D.; Zhang, Y.; Dong, P. Effect of modified atmosphere packaging on shelf life and bacterial community of roast duck meat. Food Res. Int. 2020, 137, 109645. https://doi.org/10.1016/j.foodres.2020.109645
[7] Koukou, L.; Devitt, T. D.; Dalgaard, P. Extensive growth and growth boundary model for non-proteolytic Clostridium botulinum – Evaluation and validation with MAP and smoked foods. Food Microbiol. 2022, 102, 103931. https://doi.org/10.1016/j.fm.2021.103931
[8] Hauzoukim, S. S.; Mohanty, B. Modified atmosphere packaging of fish and fishery products: A review. J. Entomol. Zool. Stud. 2020, 8(4), 651–659.
[9] Bassey, A. P.; Chen, Y.; Zhu, Z.; Odeyemi, O. A.; Gao, T.; Olusola, O. O.; Ye, K.; Li, C.; Zhou, G. Evaluation of spoilage indexes and bacterial community dynamics of modified atmosphere packaged super-chilled pork loins. Food Control 2021, 130, 108383. https://doi.org/10.1016/j.foodcont.2021.108383
[10] Ameer, A.; Seleshe, S.; Kang, S. K. Effect of Modified Atmosphere Packaging Varying in CO2 and N2 Composition on Quality Characteristics of Dry Fermented Sausage during Refrigeration Storage. Food Sci. Anim. Resour. 2022, 42(4), 639–654. https://doi.org/10.5851/kosfa.2022.e27
[11] Yang, Z.; Liu, S.; Sun, Q.; Zheng, O.; Wei, S.; Xia, Q.; Ji, H.; Deng, C.; Hao, J.; Xu, J. Insight into muscle quality of golden pompano (Trachinotus ovatus) frozen with liquid nitrogen at different temperatures. Food Chem. 2022, 374, 131737. https://doi.org/10.1016/j.foodchem.2021.131737
[12] Zhao, C. C.; Eun, J. B. Characterization of volatile compounds and physicochemical properties of hongeo using headspace solid-phase microextraction and gas chromatography-mass spectrometry during fermentation. Food Biosci. 2021, 44, 101379. https://doi.org/10.1016/j.fbio.2021.101379
[13] Khodanazary, A. Effects of Carboxymethyl Chitosan/Pectin Coating Containing Free and Nanoliposome Mentha piperita Essential Oil on the Shelf Life of Shrimp During Ice Storage. Food Sci. Nutr. 2025, 13(1), 70184. https://doi.org/10.1002/fsn3.70184
[14] Kandeepan, G.; Tahseen, A. Modified Atmosphere Packaging (MAP) of Meat and Meat Products: A Review. J. Packag. Technol. Res. 2022, 6 (3), 137–148. https://doi.org/10.1007/s41783-022-00139-2
[15] Das, K. C.; Alice, E. J.; Hossain, M. A.; Mehbub, M. F.; Islam, M. T. Effects of vacuum and modified atmosphere packaging on the shelf life of Rohu fish (Labeo rohita) stored at refrigerated temperature (4 °C). Int. Food Res. J. 2021, 28(3), 586–593. https://doi.org/10.47836/ifrj.28.3.18
[16] Li, N.; Zhang, Y.; Wu, Q.; Gu, Q.; Chen, M.; Zhang, Y.; Sun, X.; Zhang, J. High-throughput sequencing analysis of bacterial community composition and quality characteristics in refrigerated pork during storage. Food Microbiol. 2019, 83, 86–94. https://doi.org/10.1016/j.fm.2019.04.013
[17] Prabhakar, P. K.; Srivasta, P. P.; Pathak, S. S.; Das, K. Mathematical Modeling of Total Volatile Basic Nitrogen and Microbial Biomass in Stored Rohu (Labeo rohita) Fish. Front. Sustain. Food Syst. 2021, 5, 669473. https://doi.org/10.3389/fsufs.2021.669473
[18] Sai-Ut, S.; Indriani, S.; Srisakultiew, N.; Kingwascharapong, P.; Rawdkuen, S.; Kaewprachu, P.; Jung, Y. H.; Zhang, W.; Pongsetkul, J. Microbial dynamics and quality of goat meat under elevated CO2 in high-O2 modified atmosphere packaging during cold storage. Food Control 2026, 179, 111577. https://doi.org/10.1016/j.foodcont.2025.111577
[19] Kontominas, M. G.; Badeka, A. V.; Kosma, I. S.; Nathanailides, C. I. Recent Developments in Spoilage Packaging Technologies. Foods 2021, 10(5), 940. https://doi.org/10.3390/foods10050940
[20] Suprapto, H.; Kumalaningsih, S.; Wignyanto, W.; Santoso, I.; Sucipto, S. Chemical and microbial changes of stingray fish (Trygon sephen) during soaking in salt solution before smoking process. Food Res. 2022, 6(5), 68–75.
[21] Milijasevic, J. B.; Milijasevic, M.; Lilic, S.; Djinovic, S. J.; Nastasijevic, I.; Geric, T. Effect of Vacuum and Modified Atmosphere Packaging on the Shelf Life and Quality of Gutted Rainbow Trout (Oncorhynchus mykiss) during Refrigerated Storage. Foods 2023, 12(16), 3015. https://doi.org/10.3390/foods12163015
[22] Bekhit, A. A.; Holman, B.; Giteru, S.; Hopkins, D. Total volatile basic nitrogen (TVB-N) and its role in meat spoilage. Trends Food Sci. Technol. 2021, 109, 280–302. https://doi.org/10.1016/j.tifs.2021.01.006
[23] Nurjanah, N.; Suwandi, R.; Hidayat, T.; Oкторомna, V. Chemical composition and amino acid profile of fresh and steamed cobia (Rachycentron canadum L.). Food ScienTech J. 2020, 2(1), 12–19. https://doi.org/10.33512/fsj.v2i1.8136
[24] Rizzo, M. Measurement of Malondialdehyde as a Biomarker of Lipid Oxidation in Fish. Am. J. Anal. Chem. 2024, 15(9), 303–332 https://doi.org/10.4236/ajac.2024.159020
[25] Nauman, K.; Jaspal, M. H.; Asghar, B.; Manzoor, A.; Akhtar, K. H.; Ali, U.; Ali, S.; Nasir, J.; Sohaib, M.; Badar, I. H. Effect of Different Packaging Atmosphere on Microbiological Shelf Life, Physicochemical Attributes, and Sensory Characteristics of Chilled Poultry Fillets. Food Sci. Anim. Resour. 2022, 42(1), 153–174. https://doi.org/10.5851/kosfa.2021.e71
[26] Jaberi, R.; Kaban, G.; Kaya, M. Effects of vacuum and high oxygen modified atmosphere packaging on physicochemical and microbiological properties of minced water buffalo meat. Asian-Australas. J. Anim. Sci. 2019, 32(3), 421–429. https://doi.org/10.5713/ajas.18.0391
[27] Moradinezhad, F.; Dorostkar, M. Effect of Vacuum and Modified Atmosphere Packaging on the Quality Attributes and Sensory Evaluation of Fresh Jujube Fruit. Int. J. Fruit Sci. 2021, 21(1), 82–94. https://doi.org/10.1080/15538362.2020.1858470
[28] Parlapani, F. F.; Michailidou, S.; Anagnostopoulos, D. A.; Koromilas, S.; Kiosa, K.; Pasentsis, K.; Psomopoulos, F.; Argiriou, A.; Haroutounian, S. A.; Boziaris, I. S. Bacterial communities and potential spoilage markers of whole blue crab (Callinectes sapidus) stored under commercial simulated conditions. Food Microbiol. 2019, 82, 325–333. https://doi.org/10.1016/j.fm.2019.03.011
[29] Esteves, E.; Guerra, L.; Aníbal, J. Effects of Vacuum and Modified Atmosphere Packaging on the Quality and Shelf-Life of Gray Triggerfish (Balistes capriscus) Fillets. Foods 2021, 10(2), 250. https://doi.org/10.3390/foods10020250