Simple Media on Growth of Dendrobium Pompadour In Vitro

Abstract

The effect of simple media on growth of Dendrobium Pompadour in vitro was studied. Protocorms and seedlings were cultured on Vacin and Went (VW), simple media 1 (SM1), simple media 2 (SM2) and simple media 3 (SM3) for 8 weeks. The results showed that all of culture media could promote growth and development of protocorm and seedling of D. Pompadour. Protocorms developed into seedlings after 8 weeks of culture in all culture medium. SM2 medium gave the highest plantlets growth at 0.830 cm/plant and pseudobulb diameter at 0.273 cm/plant. SM3 medium gave the highest leaf number, root number and fresh weight at 5.7 leaves/plant, 11.4 roots/plant and 0.657 g/ plant, respectively after 32 weeks of culture. After transferring seedlings of D. Pompadour to greenhouse and acclimatization for 16 weeks, the seedlings from SM2 and SM3 gave the highest survival rate at 80%. The costs of simple medium (SM1, SM2 and SM3) are two times cheaper than VW medium. Thus, these culture media can be used instead of VW medium.