Wound Healing Property and Cytotoxicity against Normal Lymphocytes from Biocompounds Produced by Brevibacillus laterosporus SA14

Abstract

Biocompounds from Brevibacillus laterosporus SA14 possessed inhibitory activity against methicillin resistant Staphylococcus aureus (MRSA), a major cause of chronic wounds. The effects of the biocompounds for wound healing of skin cells (HaCaT) was studied using wound closure assay. Cytotoxicity of biocompounds against white blood cell (WBC) was also monitored using MTT assay. It was found that biocompounds from Brev. laterosporus SA14 prepared by precipitation with 80% ammonium sulfate at a concentration of 40 μg protein/ml causes the movement of HaCaT cells faster than HaCaT cells cultured in Luria-Bertani medium. Interestingly, the biocompounds at concentration of 10-80 μg/ml did not show cytotoxic effect to WBC and can stimulate WBC proliferation. These biocompounds have a potential to be used as a prototype compound for the treatment of wounds in the future.