Comparative Analysis of Bacteria Causing Bovine Mastitis by Culture Dependent Antimicrobial Resistance and Molecular Technique

Abstract

The objective of this study were to isolate S. aureus S. agalacctiae E. coli and Corynebacterium spp. from bovine-mastitis milk that were collected from small dairy farm at Thaksin University 10 samples. Ninety-five isolates of Staphylococcus were randomized collected to examine S. aureus by catalase and coagulase productions. Thirteen isolates (18.57%) were S. aureus. One hundred-three isolates of Streptococcus were examined by catalase and esculin hydrolysis production that found seven isolates (8.97%) of S. agalactiae. Isolated S. aureus could resist to Penicillin G (84.62%), Kanamycin (46.15%) while these isolates were susceptible to Cephalothin (100%) and Gentamicin (92.3%). The all of isolated S. agalactiae resisted to Penicillin G Kanamycin Cephalothin and Gentamicin. The bacterial diversity in both samples were examined by 16S rDNA-PCR and DGGE. Serratia sp. and Alcaligenes sp. were found to be dominant species.