Biofilm Formation and Adhesin Genes in Staphylococcus aureus Strains Isolated from Community and Hospital Settings in Phatthalung Province, Thailand

Abstract

Biofilm formation and surface proteins are associated with Staphylococcus aureus pathogenesis. This study, 21 MSSA and 14 MRSA isolates from community and hospital settings in Phatthalung were determined for biofilm production and the presence of icaAD, fn bA and cna genes. Biofilm was detected in 30 (85.71%) and 32(91.43%) isolates by congo red agar (CRA) and microtiter plate (MTP), respectively. Both methods correlated in 31 (88.57%) isolates. The icaAD gene was detected in 28(80%) isolates. Unexpectedly, 4 (11.43%) and 4 (11.43%) isolates were icaAD negative while MTP and CRA positive, respectively. Only 15 (42.86%) strains were found to be cna positive. Conversely, the fnbA gene was present in almost of isolates.icaAD and fnbA were found to be present in almost of isolates tested indicating a high prevalence, and these genes were equally distributed among MSSA and MRSA. Finally, there was no difference in adhesin genes distribution among community and clinical strains.