การประเมินความเป็นพิษของยาบำรุงเลือดต่อเซลล์ลิมโฟซัยท์ของมนุษย์

Abstract

          The purpose of this research is to evaluate the toxicity potenlial of Thai emmenagogue drugs by metaphase chromosome analysis of human peripheral blood lymphocytes. Ten healthy female blood samples of age between 20-35 yean old were used. Six popular brands of Thai emmenagogue drugs were tested with human peripheral blood lymphocytes and mitomycin C was used a positive control. The result showed that I out of 6 brands when the concenbation reached to zo times caused culture toxicity manifesting as chromosome contraction. In addition, at high drug concentmtion (2, 4, 1o and 20 times), the declining in mitotic indices were demonstrated with a dose response relationship. Statistically in significant differences between test and control cultures al o.o5 level showrld that no drug could induce chromosome aberration. Thus, it can conclude that when testing with humur peripheral blood lymphocytes,
the emmenagogue drugs have no clastogenic property.