Using a Smart Phone Camera as a Color Detector for Ethanol Estimation based on Time Analysis in Mixed Alcoholic Drinks

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Narong Kotchabhakdi


The colorimetric method for ethanol estimation was developed based on the redox reaction of chromium using a smartphone camera as a color detector. Time-Based Analysis (TBA) was employed for color signal evaluation. The Red Green Blue (RGB), Cyan Magenta Yellow (CMY) and Hue saturation Value (HSV) color models were used for monitoring a color change from Orange (Cr(VI)) to Blue (Cr (III)) in a reaction plate-form. The reaction time of ethanol and dichromate ion depended on the concentration of ethanol in which the stop time of each color model was selected and recorded in the second unit and employed for signal processing in log scale. The results showed a linear range of 1.0-5.0 %w/v ethanol, r2= 0.991-0.998, % recovery of 82-118% and %RSD less than 10% of analysis performance test with mixed alcoholic drink samples. The proposed method was successfully applied for determination ethanol in real samples with less complicated, rapid, low cost and portable.

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Cabanero, A.I., Recio, J.L. & Ruperez, M. (2010). Simultaneous stable carbon isotopic analysis of wine glycerol and ethanol by liquid chromatography coupled to isotope ratio mass spectrometry. Journal of Agricultural and Food Chemistry, 58(2), 722-728.

Diaz, A.N., Sanchez, F.G., Baro, E.N., Diaz, A.F., Aguilar, A. & Algarra, M. (2012). Sensitive chemiluminescent immunoassay of triclopyr by digital image analysis. Talanta, 97, 42-47.

Devadhasan, J.P. & Kim, S. (2015). An ultrasensitive method of real time pH monitoring with complementary metal oxide semiconductor image sensor. Analytica Chimica Acta, 858, 55-59.

Jayawardane, B.M., Wongwilai, W., Grudpan, K., Kolev, S.D., Heaven, M.W., Nash, D.M. & McKelvie, I.D. (2014). Evaluation and application of a paper-based device for the determination of reactive phosphate in soil solution. Journal of Environmental Quality, 43 (3), 1081-1085.

Klasner, S.A., Price, A.K., Hoeman, K.W., Wilson, R.S., Bell, K.J. & Culbertson, C.T. (2010). Paper-based microfluidic devices for analysis of clinically relevant analytes present in urine and saliva. Analytical and Bioanalytical Chemistry, 397(5), 1821-1829.

Li, H., Chai, X.S., Deng, Y., Zhan, H. & Fu, S. (2009). Rapid determination of ethanol in fermentation liquor by full evaporation headspace gas chromatography. Journal of Chromatography A,1216(1), 169-172.

Li, X., Jia, G., Cao, Y., Zhang, J., Wang, L. & Sun, H. (2013). Simultaneous determination of delta13C values of glycerol and ethanol in wine by liquid chromatography coupled with isotope ratio mass spectrometry. Chinese Journal of Chromatography, 31(12), 1201-1205.

Moonrungsee, N., Pencharee, & Jakmunee, J. (2015). Colorimetric analyzer based on mobile phone camera for determination of available phosphorus in soil. Talanta, 136, 204-209.

Nascimento, E.S., Cardoso, D.R. & Franco, D.W. (2008). Quantitative ester analysis in cachaca and distilledspirits by gas chromatography-mass spectrometry (GC-MS). Journal of Agricultural and Food Chemistry, 56(14), 5488-5493.

Neves, L.A., Rodrigues, J.M., Daroda, R.J., Silva, P.R., Ferreira, A.A., Aranda, D.A., Eberlin, M.N. & Fasciotti, M. (2015). The influence of different referencing methods on the accuracy of delta(13) C value measurement of ethanol fuel by gas chromatography/combustion/ isotope ratio mass spectrometry. Rapid Communications in Mass Spectrometry, 29(21), 1938-1946.

Semichon, L. & Flanzy, M. (1929). Determination of alcohol in wines and liquors by means of potassium dichromate and sulfuric acid. Annales des Falsifications et des Fraudes, 22, 139-152.

Songjaroen, T., Dungchai, W., Chailapakul, O. & Laiwattanapaisal, W. (2011). Novel, simple and low-cost alternative method for fabrication of paper-based Microfluidics by Wax Dipping. Talanta, 85(5), 2587-2953.

Williams, M.B. & Darwin, R. (1950). Colorimetric determination of ethyl alcohol. Analytical Chemistry, 22 (12), 1556-1564.

Wongwilai, W., Lapanantnoppakhun, S. & Grudpan, K. (2010). Webcam camera as a detector for a simple lab-on-chip time based approach.Talanta, 81(3), 1137-1141.

Zhang, C.Y., Lin, N.B., Chai, X.S., Zhong, L. & Barnes, D.G. (2015). A rapid method for simultaneously determining ethanol and methanol content in wines by full evaporation headspace gas chromatography. Food Chemistry, 183, 169-172.

Zimmerman, H.W. (1963). Studies on the dichromate method of alcohol determination. The American Journal of Enology and Viticulture, 14, 205-213.