Colorimetric Assay Based on Green Analytical Method for Determination of Acetic Acid Using Red Cabbage (Brassica oleraceae L. var.) as a Natural Reagent

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Yaowalak Khanhuathon


A green portable micro-scale colorimetric technique was developed for acetic acid determination. This system is controlled via an application on mobile phone. Furthermore, red cabbage (Brassica oleraceae L. var.) extracts were used as an alternative natural reagent for the quantification of acetic acid. The purple color of cyanidin contained in the red cabbage extracts reacted with acetic acid to produce a magenta color substance and showed maximum absorption wavelength at 525 nm. The various parameters for optimum condition were investigated. Under the suitable condition, a linear calibration graph in the range of   1.00-5.00 %w/v acetic acid (y = 0.063x + 0.142, R2 = 0.9777) was obtained with LOD and LOQ of 0.03 and 0.20 %w/v, respectively. The tests were produced 6 times (n = 6) to ensure the precision of the proposed technique, and showed 3.4, 2.2, and 1.0 %RSD for 1.00, 3.00, and 5.00 %w/v of acetic acid, respectively. The proposed method was successfully applied to determine acetic acid in vinegar samples. The results agreed well with the standard method with a non-significant difference at 95% confidence interval. The proposed method presented many advantages such as using a cost-effective and environmental friendly analysis system, less toxic and producing less waste.

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