สารสกัดจากใบกระถินที่ถูกตรึงบนกระดาษสำหรับเป็นชุดทดสอบปริมาณไอออนเหล็ก ในตัวอย่างยาเสริมธาตุเหล็ก

Yaowalak  Khunhuathon; Narong  Kotchabhakdi; Napaporn Wannaprom

Authors

Yaowalak Khunhuathon Chemistry Program, Faculty of Education, Chiang Rai Rajabhat University
Narong Kotchabhakdi Chemistry Program, Faculty of Science, Lampang Rajabhat University
Napaporn Wannaprom Chemistry Program, Faculty of Education, Chiang Rai Rajabhat University https://orcid.org/0009-0006-8021-1730

Keywords:

Iron ion, Leucaena leucocephala (Lam) de Wit leaf extracts, Natural reagent, Lab-on-paper, Iron supplement

Abstract

The iron ion determination in iron supplement using the lab-on-paper technique with Leucaena leucocephala (Lam) de Wit leaf extracts as a reagent and RGB color measurement via mobile phone was developed. The reaction between mimosine in Leucaena leucocephala (Lam) de Wit leaf extracts and iron ions was provided by a brick-orange complex compound which its highest light absorption spectrum at 460 nm. The various parameters were examined in order to obtain the appropriate condition were studied. The optimum values were found to be the concentration of acacia leaf extract at 2.5 % w/v, extraction time of 20 minutes, pH in the reaction of 4.5, volume of sample solution dropped on paper of 15 mL, reaction time of 7.5 minutes, and blue light (B) used as monitoring of the signals. Under optimum conditions, it was found that the linearity range of the iron ion standard solution was in the range of 1.00–10.00 ppm (y = 0.9626x–2.1379, R² = 0.9959). LOD and LOQ values were 0.09 and 3.68 ppm, respectively. The accuracy of the proposed method was studied at iron ion concentrations of 1.00, 5.00 and 10.00 ppm (n = 8). The results showed that the %RSD was 3.00, 2.84 and 2.38, respectively. The % recovery was 87–93%. The iron ion determination in iron supplements by the proposed method and the standard method were compared using t-test statistical analysis values at the 95% confidence level. It was found that there were no significant differences. This research can be considered a simple, environmentally friendly and economical.

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