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This research studies the production of bacterial cellulose from Acetobacter xylinum TISTR 975 with pineapple peel juice, a low cost carbon source, and coconut water as nutrient and carbon source. The factors influencing the production were studied including incubation time, type of carbon sources and ratio of mixed carbon sources. In addition, the characteristics of the produced bacterial cellulose were investigated using Fourier Transform Infrared Spectrophotometer (FTIR), Scanning Electron Microscope (SEM), Thermogravimetric Analyzer (TGA), Differential Scanning Colorimeter (DSC) and X-ray Diffractometer (XRD). It was found that the coconut water produced the amount of bacterial cellulose higher than the pineapple peel juice with 10 days of production. An addition of the coconut water into the pineapple peel juice increased the amount of bacterial cellulose. The characteristics of two bacterial celluloses from two different sources were nearly similar but the one from the pineapple peel juice had higher impurities. It can be concluded that the pineapple shell extract could be used as low cost carbon source for bacterial cellulose production.
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