Production and characterization of bacterial cellulose produced by Acetobacter xylinum TISTR 975 from pineapple peel juice

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adisak Jaturapiree
Ekrachan Chaichana
Thanunya Saowapark
Bongkot Chuenpraphai
Phimchanok Jaturapiree

Abstract

This research studies the production of bacterial cellulose from Acetobacter xylinum  TISTR 975 with pineapple peel juice, a low cost carbon source, and coconut water as nutrient and carbon source. The factors influencing the production were studied including incubation time, type of carbon sources and ratio of mixed carbon sources. In addition, the characteristics of the produced bacterial cellulose were investigated using Fourier Transform Infrared Spectrophotometer (FTIR), Scanning Electron Microscope (SEM), Thermogravimetric Analyzer (TGA), Differential Scanning Colorimeter (DSC) and X-ray Diffractometer (XRD). It was found that the coconut water produced the amount of bacterial cellulose higher than the pineapple peel juice with 10 days of production. An addition of the coconut water into the pineapple peel juice increased the amount of bacterial cellulose. The characteristics of two bacterial celluloses from two different sources were nearly similar but the one from the pineapple peel juice had higher impurities. It can be concluded that the pineapple shell extract could be used as low cost carbon source for bacterial cellulose production.

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บทความวิจัย (Research Articles)

References

[1] R. Jonas and L.F. Farah, “Production and application of microbial cellulose,” Polymer Degradation and Stability, vol. 59, pp. 101-106, 1998.
[2] H.C. Huang, L.C. Chen, S.B. Lin, C.P Hsu and H.H. Chen, “In situ modification of bacterial cellulose network structure by adding interfering substances during fermentation,” Bioresource Technology, vol. 15, pp. 6084-91, 2010.
[3] S.-P. Lin, C. I. Loira, J. M Catchmark, J.-R. Liu, A. Demirci and K.-C. Cheng, “Biosynthesis, production and applications of bacterial cellulose,” Cellulose, vol. 20, no. 5, pp. 2191-2219, 2013.
[4] E.P. Çoban and H. Biyik, “Effect of various carbon and nitrogen sources on cellulose synthesis by Acetobacter lovaniensis HBB5,” African Journal of Biotechnology, vol.10, no. 27, pp.5346-5354, 2011.
[5] A. Kurosumi, C. Sasaki, Y. Yamashita and Y. Nakamura, “Utilization of various fruit juices as carbon source for production of bacterial cellulose by Acetobacter xylinum NBRC 13693,” Carbohydrate Polymers, vol. 76, pp.333–335, 2009.
[6] M. Wichitta, K. Pichamol, S. Suwanna, P. Kullanan, A. Chutima, P. Jantima, and L. Surasak Laloknam, “Production of Bacterial Cellulose by Acetobacter xylinum TISTR086 using Agricultural Products as Carbon Sources,”Journal of Research Unit on Science, Technology and Environment for Learning , vol 3 , no. 2, pp. 92-97, 2012.
[7] P. Kriangkrai, P. Arunya and S. Wanticha, " Characterization of bacterial cellulose (Nata de coco) from pitaya , Khon Kaen Agriculture Journal, Vol.43 Supplement, pp. 917 -921, 2015.
[8] P. Chintana, Y. Wanida and K. Julaluk, “A Study of the Optimal Fermentation Conditions for Nata de Coco Production by Acetobacter xylinum TISTR 975 from Mango Juice,” KMUTT Research and Development Journal, vol. 40, no. 2, pp. 272-281, 2017.
[9] N. Tyagi and S.Suresh, “Production of cellulose from sugarcane molasses using Gluconacetobacter: optimization and characterization,”Journal of Cleaner Production, vol.112, pp.71–80, 2016.
[10] S. M. A. Keshk, M. A.T. Razek and K. Sameshima, “Bacterial Cellulose Production from Beet Molasses,” African Journal of Biotechnology, vol. 5, no.17, pp. 1519-1523, 2006.
[11] C. Moukamnerd, S. Saenchang, A. Krutjan and C. Techapun, “Production of bacterial cellulose by Acetobacter xylinum using wastewater from pineapple processing as a carbon source,” Khon Kaen Agriculture Journal. vol. 46, no.3, pp.581-590, 2018.
[12] E. Tsouko, C. Kourmentza, D. Ladakis, N. Kopsahelis, I. Mandala, S. Papanikolaou, F. Paloukis, V. Alves, A. Koutinas, “Bacterial cellulose production from industrial waste and by-product streams,” International Journal of Molecular Sciences, vol.16, no.7, pp.14832-14849, 2015.
[13] K. Chalermkiet, O. Sompong and P. Nantharat, “Cellulose Production from Oil Palm Shoot Juices Felled for Replanting by Acetobacter xylinum TISTR 086,” in proceeding of the 54th Kasetsart University Annual Conference, Thailand, pp. 102 -109, 2016.
[14] G. Pacheco, C.R. Nogueira, A. Meneguin, E. Trovatti, C.C.M. Silva, R. Machado, JR.S. Ribeiro, E.C. da Silva Filho and H. Da Silva Barud,“Development and characterization of bacterial cellulose produced by cashew tree residues as alternative carbon source,” Industrial Crops and Products, vol. 107, pp.13-19, 2017.
[15] M. Dubois, K. A. Gilles, J. K. Hamilton, P. A. Rebers and F. Smith, “Colorimetric method for determination of sugars and related substances,” Analytical Chemistry, vol. 28, pp.350-356. 1956.
[16] S. Sheykhnazari, T. Tabarsa, A. Ashori, A. Shakeri and M. Golalipour, “Bacterial synthesized cellulose nanofibers; Effects of growth times and culture mediums on the structural characteristics,” Carbohydrate Polymers. Vol.86, pp.1187–1191, 2011.
[17] P. Carreira, J.A.S. Mendes, E. Trovatti, L.S. Serafim, C.S.R. Freire, A.J.D. Silvestre and C.P. Neto, “Utilization of residues from agro-forest industries in the production of high value bacterial cellulose,” Bioresource Technology, vol. 102, pp. 7354–7360, 2011.
[18] F. Mohammadkazemi, M. Azin and A. Ashori, “Production of bacterial cellulose using different carbon sources and culture media,” Carbohydrate Polymer, vol. 117, pp. 518–523, 2015.
[19] F.P. Gomes, N.H.C.S. Silva, E. Trovatti, L.S. Serafim, M.F. Duarte, A.J.D. Silvestre, C.P. Neto, C.S.R. Freire,“Production of Bacterial Cellulose by Gluconacetobacter sacchari using Dry Olive Mill Residue,” Biomass and Bioenergy, vol. 55, pp. 205-211, 2013.
[20] M.M. Lapuz, E.G. Gallardo and M.A. Palo, “The nata organism cultural requirements, characteristics and identity,” Philippine Journal of Science, vol. 96, pp. 91- 108, 1967.
[21] C. Kanjana, and S. Rutairat, “Development of bacterial cellulose production in molasses by using coconut juice as the nutrient supplement and adding of gelling agent” RMUTSB Academic journal, vol. 3, no. 2, pp. 98-108, 2015.
[22] P. Singhsa, R. Narain and M. Manuspiya, “Bacterial Cellulose Nanocrystals (BCNC) Preparation and Characterizations from Three Bacterial Cellulose Sources, and Development of Functionalized BCNC as Nucleic Acid Delivery Systems,” ACS Applied Nano Materials. Vol.1, pp. 209-221, 2018.
[23] S.M. Yim, J.E. Song and H.R. Kim, “Production and characterization of bacterial cellulose fabrics by nitrogen sources of tea and carbon sources of sugar,” Process Biochemistry. Vol. 59, pp. 26-36, 2017.
[24] T. Tabarsa, S. Sheykhnazari, A. Ashori, M. Mashkour, and A. Khazaeian, “Preparation and characterization of reinforced papers using nano bacterial cellulose”. International journal of biological macromolecules. 101, 334-340. 2017.
[25] Y.Jia, X. Wang, M. Huo, X. Zhai, F. Li and C. Zhong, “Preparation and characterization of a novel bacterial cellulose/chitosan bio-hydrogel,” Nanomaterials and Nanotechnology, vol. 7, pp. 1-8, 2017.
[26] S. Gea, C. T. Reynolds, N. Roohpour, B. Wirjosentono, N. Soykeabkaew and E. Bilotti, Investigation into the structural, morphological, mechanical and thermal behaviour of bacterial cellulose after a two-step purification process” Bioresource Technology, vol. 102, pp. 9105–9110. 2011.
[27] Vasconcelos, N.F., Feitosa, J.P.A., da Gama, F.M.P., Andrade, F.K., de Souza Filho, M.S. and M.F. Rosa, “Bacterial cellulose nanocrystals produced under different hydrolysis conditions: Properties and morphological features,” Carbohydrate Polymers. Vol. 155, pp. 425-431.
[28] Surma-Slusarska B., Presler S., Danielewicz D. (2008). “Characteristics of Bacterial Cellulose Obtained from Acetobacter xylinum Culture for Application in Papermaking”. Fibre & Textiles in Eastern Europe.16, 108-111
[29] X. Fan, Y. Gao, W. He, H. Hu, M. Tian, K. Wang and S. Pan, “Production of nano bacterial cellulose from beverage industrial waste of citrus peel and pomace using Komagataeibacter xylinus,” Carbohydrate Polymers, vol. 151, pp. 1068-1072, 2016.
[30]. W. Czaja, D. Romanovicz and R. M. Brown, “Structural investigations of microbial cellulose produced in stationary and agitated culture” Cellulose, Vol. 11, pp. 403–411, 2004.