Effects of Planting Media and Plant Care Practices on Ex Vitro Rooting of Philodendron pedatum (Hook.) Kunth
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Abstract
Philodendron pedatum (Hook.) Kunth is widely used as an ornamental plant for cutting leaf, gardening, or pot plant to decorate buildings. The plant tissue culture technique is employed to propagate this economically important crop for the high demand of the ornamental plant industry. However, the transplantation of in vitro rooted plants frequent results in plant death, which is causing a significant huge loss in plant propagation resource. This research aimed to evaluate the ex vitro rooting induction of P. pedatum. In vitro young shoots, 1 cm in height with 3 leaves, were cultured on Murashige and Skoog (MS) agar medium and compared with in vitro-raised plants grown under ex vitro conditions on peat moss (10 plants/condition with 3 times repeated). The results showed that all plants grown ex vitro were able to survive and rooting. Additionally, ex vitro plants exhibited data of new root number, new root length, and overall fresh weight significantly higher than in vitro plants. Planting media (peat moss, coco peat, a mixture of 1 peat moss : 1 coco peat) and plant care substance application (distilled water, MS liquid medium, Flavogen®, Osil®) were subsequently studied to identify the most suitable substrate for ex vitro rooting (10 plants/treatment with 3 times repeated). The findings revealed that using peat moss as the planting material combined with Flavogen®, an agrochemical, as the plant care substance resulted in the highest shoots and roots growth. This research will solve the problem of plant death after transplantation and could help reduce the cost of propagating P. pedatum using plant tissue culture techniques.
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ลิขสิทธ์ ของมหาวิทยาลัยเทคโนโลยีราชมงคลพระนครReferences
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