การเพิ่มคุณสมบัติพรีไบโอติกของกากถั่วเหลืองโดยใช้เอนไซม์ที่เหมาะสม

Premsuda Saman; Nanthida Khamkongkaew

Authors

Premsuda Saman Biodiversity Research Center, Thailand Institute of Scientific and Technological Research
Nanthida Khamkongkaew Biodiversity Research Center, Thailand Institute of Scientific and Technological Research

Abstract

This research aimed to study the prebiotic properties of dietary fiber obtained from soybean residue hydrolyzed with appropriate enzymes. Soybean meal was treated using five commercial enzymes: alpha-amylase, cellulase, hemicellulase, mannanase, and xylanase. The results showed that the digestion of soybean residue with cellulase yielded the highest amount of reducing sugar. When studying the composition of the sugars produced using the thin layer chromatography (TLC) technique, it was found that the color bands that appeared on TLC plate had a distance similar to that of glucose, fructose, arabinose and sucrose. The prebiotic activity of cellulase-treated soybean residue solutions was investigated using two probiotic strains, Bifidobacterium animalis TISTR 2194 and B. bifidum TISTR 2195 while Escherichia coli TISTR 887 was tested as a comparative inoculum. Results showed that the solution obtained from cellulase-treated soybean residue could promote the growth of B. animalis and B. bifidum. As a result, the prebiotic activity score of the cellulase-treated soybean residue solution was positive and significantly higher than those of the untreated soybean residue solution.

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Enhancement of prebiotic properties of soybean residue using appropriate enzymes

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