Anatomical Characteristics of the Leaf Blade of Medicinal Plants in the Family Menispermaceae in Roi Et Province
Keywords:
Leaf epidermis, Medicinal plants, Menispermaceae, Roi Et ProvinceAbstract
This study investigated the leaf anatomical characteristics of 9 medicinal plant species belonging to 6 genera within the family Menispermaceae in Roi Et Province. The objectives were to describe their anatomical features, construct a taxonomic key for species identification, and establish a regional database. Leaf samples were prepared using epidermal peeling and clearing methods and examined under a light microscope. The results revealed key diagnostic characters for species identification, including: 1) epidermal cell shapes (polygonal, irregular, and jigsaw-like); 2) stomatal types (anomocytic, pentacytic, and tetracytic); 3) trichome types (unicellular, clavate unicellular, and glandular); 4) cuticle ornamentations (smooth, papillose, and striated); and 5) types of cell inclusions (prismatic, raphide, and druse crystals, oil cells, and red-staining substances). Quantitative analysis showed that the stomatal index ranged from 11.02 to 26.33%, while the guard cell length ranged from 21.7 to 32.1 µm. These values exhibited clear interspecific variation and can be used as statistically significant supporting characters for species identification. These anatomical characteristics reflect interspecific variability and enable the construction of an effective identification key that can clearly distinguish all 9 species. The findings provide important diagnostic characters for the classification and identification of plants in the family Menispermaceae.
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