Sex Predetermination by semen sexing in bovine
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Abstract
This academic paper is compiled based on past research that has attempted to isolate sperm to have specific X or Y chromosomes, which, when applied, would result in a calves that meet the needs of farmers. The most accurate sperm screening method is flow cytometry / cell sorter. This method is based on the principle that sperm with X have approximately 4% more DNA content than sperm with Y chromosome. Sperm isolation was performed DNA stained with Hoeschst 33342, which gave a blue glow when receiving a laser light with a wavelength of 351 - 364 nm. Sperms with an X chromosome glow more than sperm with the Y chromosome when the dyed sperm was introduced into the machine. sorter Sperm with X chromosome, Y chromosome, can be separated with approx 90% screening accuracy. The sorter is able to sort 32,000 sperm / sec. After the separation process, 8,000 sperm with X and Y chromosomes are obtained. Semen / second. If containing 2 million semen tubes per tube, 14 ampoules can be produced / each sex. In an hour This method can be used for commercial purposes. Separate semen can be used for insemination of both dairy cattle and beef cattle. It is more suitable than used in the mother cows. Simultaneous induction of ovulation (FTAI) may be performed to facilitate the management of IVF. Gender separated semen is suitable for embryo production prior to transfer in order to obtain calves of the sex they need. The use of separate sexes shortened the time for cattle breeding. And resulting in increased income within the farmers' farms.
Article Details
เนื้อหาและข้อมูลในบทความที่ลงตีพิมพ์ในวารสารวารสารวิทยาศาสตร์และเทคโนโลยีถือเป็นข้อคิดเห็นและความรับผิดชอบของผู้เขียนบทความโดยตรงซึ่งกองบรรณาธิการวารสาร ไม่จำเป็นต้องเห็นด้วย หรือร่วมรับผิดชอบใด ๆ
บทความ ข้อมูล เนื้อหา รูปภาพ ฯลฯ ที่ได้รับการตีพิมพ์ในวารสารวารสารวิทยาศาสตร์และเทคโนโลยีถือเป็นลิขสิทธิ์ของวารสารวารสารวิทยาศาสตร์และเทคโนโลยีหากบุคคลหรือหน่วยงานใดต้องการนำทั้งหมดหรือส่วนหนึ่งส่วนใดไปเผยแพร่ต่อหรือเพื่อกระทำการใด ๆ จะต้องได้รับอนุญาตเป็นลายลักษณ์อักษรจากวารสารวารสารวิทยาศาสตร์และเทคโนโลยี ก่อนเท่านั้น
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