First Karyotypic Study of the Kissing Gouramies, Helostoma temminckii (Anabantiformes, Helostomatidae) from Thailand by Conventional and Ag-NOR staining techniques
Keywords:
Helostoma temminckii, karyotype, idiogram, chromosomeAbstract
The first chromosomal study of kissing Gouramies, Helostoma temminckii was karyotypically performed from Thailand. Kidney cells were obtained from ten of male and female specimens. Then, conventional staining and Ag-NOR banding were carried out to stain the chromosomes. The results of conventional staining mainly demonstrated telocentric chromosome with diploid number (2n) and fundamental number (NF) as 48. While, Ag-NOR banding technique presented the position of nucleolar organizer regions (NORs) at the end of telomere of chromosome pair four. Karyotype consisted of 16 large, 30 medium and two small telocentric chromosomes. Thus, the karyotype formula represented as follows: Lt16+Mt30+St2.
References
Abu-Almaaty, A.H., Hassan, M.K., Bahgat, I.M. & Suleiman, M.E.E. (2017). Inter simple sequence repeat (ISSR) and cytogenetic analysis of three fish species of family osphronemidae. Egyptian Journal of Aquatic Biology and Fisheries, 21 (2), 1-15.
Ahmadi. (2021). Length-weight relationship and relative condition factor of the Kissing Gourami (Helostoma temminckii) from Sungai Batang River, Indonesia. Songklanakarin J. Sci. Technology, 43 (1), 210-217.
Amemiya, C.T. & Gold, J.R. (1986). Chromomycin A 3 Stains Nucleolus Organizer Regions of Fish Chromosome. Copeia, 1986 (1), 226.
Arai, R. (2011). Fish karyotypes: a check list. Springer.
Arifin, O.Z., Cahyanti, W., Kristanto & A.H., (2017). Genetic diversity of three generations of kissing gourami (Helostoma temminckii) under a domestication program. Jurnal Riset Akuakultur,12 (4), 295-305.
Asyari, E. (2007). Food habit of Biawan fish (Helostoma temminckii) at Sababila, Barito South Kalimantan. Journal of Protein, 14, 1-11.
Baimai, V. (2010). Biodiversity in Thailand. The Journal of the Royal Institute of Thailand, 2, 107-118.
Chaiyasan, P., Mingkwan, B., Jantarat, S., Suwannapoom, C., Cioffi, M.B., Liehr, T., Talumphai, S., Tanomtong, A. & Supiwong, W. (2021). Classical and molecular cytogenetics of Belontia hasselti (Perciformes: Osphronemidae): Insights into the ZZ/ZW sex chromosome system. Biodiversitas, 22 (2), 548-556.
Chaiyasan, P., Supiwong, W., Saenjundaeng, P., Seetapan, K. Pinmongkhonkul, S., Tanomtong, A. (2018). A report on classical cytogenetics of highfin Barb fish, Cyclocheilichthys armatus (Cypriniformes, Cyprinidae). Cytologia, 83 (2), 149-154.
Chaiyasut, K. (1989). Cytogenetics and Cytotaxonomy of the Family Zephyranthes. Department of Botany, Chulalongkorn University.
Coates, D., Ouch, P., Suntornratana, U., Nguyen, T.T. & Viravong, S. (2003). Biodiversity and fisheries in the Mekong River Basin. Mekong Development Series, 2, 1-30.
Crocker, J. (1990). Nucleolar organiser regions. Current Topics in Pathology Ergebnisse Der Pathologie, 82, 91-149.
Donsakul, T., Magtoon, W. & Rungsiruji, A. (2006). Karyotypes of three giant goramy, Osphronemus goramy, O. exodon and O. laticlavius (Pisces: Osphronemidae). Proceedings of 44th Kasetsart University Annual Conference Fisheries (pp. 461- 468). Bangkok.
Donsakul, T., Magtoon, W. & Rungsiruji, A. (2009). Karyotypes of five belontiid fishes (Family Belongtiidae): Betta smaragdina, B. simplex, B. sp., Trichopsis pumila and T. Schalleri. The 35th Congress on Science and Technology of Thailand (pp.77). Chonburi, Thailand.
Esmaeili, H.R., Pirvar, Z., Ebrahimi, M. & Matthias, F. (2015). Karyological and molecular analysis of three endemic loaches (Actinopterygii: Cobitoidea) from Kor River basin, Iran. Molecular Biology Research Communications, 4 (1), 1-13.
Ferry, L.A., Konow, N. & Gibb, A.C. (2012). Are Kissing Gourami Specialized for Substrate-Feeding? Prey Capture Kinematics of Helostoma temminckii and Other Anabantoid Fishes. Journal of Experimental Zoology Part A: Ecological Genetics and Physiology, 317 (9), 571-579.
Furgala-Selezniow, G., Fopp-Bayat, D., Jankun, M., Krejszeff, S. & Mamcarz, A. (2008). Note on the karyotype and NOR location of Siamese fighting fish Betta splendens (Perciformes, Osphronemidae). Caryologia, 61 (4), 349-353.
Getlekha, N. & Tanomtong, A. (2020). First Cytogenetic Study of Green Chromis, Chromis viridis (Perciformes, Pomacentridae) by Conventional Staining and Ag-NOR Banding Techniques. Chiang Mai University Journal of Natural Sciences, 19 (2), 164-175.
Hinegardner, R. & Rosen, D.E. (1972). Cellular DNA content and the evolution of teleostean fishes. The American Naturalist, 106 (951), 621-644.
Howell, W.M. & Black, D.A. (1980). Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia, 36, 1014-1015.
Jantarat, S., Supiwong, W., Phintong, K., Sonsrin, K., Kong-ngarm, N. & Tanomtong, A. (2017). First Analysis on the Cytogenetics of Painted Sweetlip, Plectorhinchus pictus (Heamulidae: Perciformes) from Thailand. Cytologia, 82 (2), 145-150.
Juntaree, S. & Supiwong, W. (2020). Standardized Karyotype and Idiogram of the Glass Goby Fish (Gobiopterus chuno) (Hamilton, 1822) in Thailand. Science Technology and Engineering Journal (STEJ), 6 (2), 72-81.
Khakhong, S., Supiwong, W., Tanomtong, A., Sriuttha, M., Jearranaiprepame, P., Soemphol, W. & Jiwyam, W. (2014). A First Chromosomal Characterization of NORs in Splendid Snakehead Fish, Channa lucius (Perciformes, Channidae). Cytologia, 79 (2), 133-139.
Khuda-Bukhsh, A.R., Rahman, A., Chanda T., Nayak, K., Khuda-Bukhsh, A.R. (1995). Diploid numbers and chromosome formulae of some 29 species of Indian teleosts (Pisces)
Kopecký, J., Král, M., Čurlej, J. & Mrázová, L. (2012). Influence of Astaxanthin and β-carotene on Kissing Gourami (Helostoma temminckii) Colouring. Scientific Papers: Animal Science and Biotechnologies, 45 (2), 57-60.
Kottelat, M. (2001). Fishes of Laos. WHT Publications Ltd.
Krysanov, E.Y. & Golubtsov, A.S. (2001). Karytypes of eight species of African climbling gouramie from the genera Ctenopoma and Microctenopoma (Anabantidae, Perciformes) and their probable phylogenetic relationships. Voprosy Ikhtiologii, 41 (9), 737-741.
Kumar, R., Nagpure, N.S., Behera, B.K. & Lakra, W.S. (2008). Chromosome banding in two freshwater Gouramies from North Eastern India. Nucleus, 51 (1,2), 61-72.
Kumar, R., Kushwaha, B., Nagpure, N.S., Behera, B.K. & Lakra, W.S. (2013). Karyological and molecular diversity in three freshwater species of the genus Channa (Teleostei, Perciformes) from India. Caryologia: International Journal of Cytology, Cytosystematics and Cytogenetics, 66 (2), 109-119.
Liem, K.F. (1967). Functional morphology of the head of the anabantoid teleost fish Helastoma temmincki. Journal of Morphology, 121, 135-157.
Magtoon, W., Rangsiruji, A. & Donsakul, T. (2007). Karyotypes of Betta splendens, B. prima, Trichopsis vittatus and Trichogaster trichopterus (family Belontiidae) from Thailand. The 33rd Congress on Science and Technology of Thailand (pp. 94), Thailand.
Muryati, S., Putra, R.M. & Efizon, D. (2016). A Study on morphometric and meristic of Helostoma temminckii from swamp area in the Bencah Kelubi Village, Tapung Kiri Sub-Regency, Kampar Regency, Riau Province. Jurnal Online Mahasiswa Fakultas Peri-kanan dan Ilmu Kelautan Universitas Riau, 3, 1-10.
Nelson, J.S., Grande, T.C., Wilson, M.V.H. (2016). Fishes of the World (5th ed). John Wiley & Sons, Inc.
Pazza, R., Kavalco, K.F., Penteado, P.R., Kavalco, S.A.F. & De AlmeidaToledo L.F. (2009). Gene mapping of 18S and 5S rDNA genes in the karyotype of the three-spot gourami Trichogaster trichopterus (perciformes, osphronemidae). Zebrafish, 6 (3), 219-222.
Phimphan, S., Tanomtong, A., Jumrusthanasan, S., Supiwong, W., Siripiyasing, P. & Sanoamuang, L. (2013). First Report of NOR Polymorphism and Chromosome Analysis of John’s Snapper, Lutjanus johnii (Perciformes, Lutjanidae) in Thailand. Cytologia, 78 (4), 335-344.
Prianto, E., Husnah, N.S. & Asyari. (2016). Food habit of Biawan fish (Helostoma temminckii) at Sababila, Barito South Kalimantan. Jurnal PROTEIN, 14 (2), 161-166.
Rainboth, W.J. (1996). Fishes of the Cambodian Mekong. Food and Agriculture Organization of the United Nation.
Rishi, S., Gill, P.K. & Rishi, K.K. (1997). Karyotypic studies in eight species of fishes belonging to family Belontiidae (Pisces, Perciformes). Journal of Chromatographic Science, 1, 55-59.
Rishi, S., Gill, P.K. & Rishi, K.K. (2001). Heterochromatin localization in the chromosomes of five belontid fishes (Belontiidae, Perciformes). Chromosome Science, 5 (1), 35-38.
Rooney, D.E. (2001). Human Cytogenetics: Constutitional Analysis: A Practical Approach. Oxford University Press.
Sakai, H.M., Wang, J.L. & Naka, K. (1995). Contrast gain control in the lower vertebrate retinas. The Journal of General Physiology, 105 (6), 815-835.
Sangpakdee, W., Phimphan, S., Tanomtong A., Patawang, I., Pinthong, K. & Neeratanaphan, L. (2015). Karyological study of Lutjanus ehrenbergii and L. carponotatus (perciformes, lutjanidae) by classical and Ag-NOR staining techniques. Nucleus (India), 58 (3), 1-7.
Sangpakdee, W., Phimphan, S., Tengjaroenkul, B., Pinthong, K., Neeratanaphan, L., Tanomtong, A. (2017). Cytogenetic study of tree microhylid species (Anura, Microhylidae) from Thailand. Cytologia, 82, 67-74.
Seetapan, K. & Khamma-Ai, M. (2007). Karyotypes of the genus Trichogaster from Thailand. The Proceedings of Fisheries Annual Conference (pp. 323-329). Bangkok.
Sobita, N. & Bhagirath, T. (2007). Colisa fasciata (Schneider) (Perciformes, Anabantoidei, Belontidae) karyotype is divergent and ZW sex chromosomes are allocyclic and asynaptic. Nucleus, 50, 45-49.
Sochorová, J., Garcia, S., Gálvez, F., Symonová, R. & Kovaří, A. (2018). Evolutionary trends in animal ribosomal DNA loci: Introduction to a new online database. Chromosoma, 127 (1), 141-150.
Sreeputhorn, K., Mangumphan, K., Muanphet, B., Tanomtong, A., Supiwong, W. & Kaewmad, P. (2017). The First Report on Chromosome Analysis of F1 Hybrid Catfish: Mekong Giant Catfish (Pangasianodon gigas)x Striped Catfish (Pangasianodon hypophthalmus) and Spot Pangasius (Pangasius larnaudii)×Pangasianodon hypophthalmus (Siluriformes, Pangasiidae). Cytologia, 82 (4), 457-463.
Supiwong, W., Tanomtong, A., Kenthao, A., Seetapan, K., Kaewsri, S. & Sanoamuang L. (2010). Standardized karyotype of the three-spot gourami, Trichogaster trichopterus (Perciformes, Belontidae) from Thailand by conventional and Ag-NOR staining technique. The Nucleus, 53 (3), 103-107.
Tan, S.H. (2006). The Raffles Bulletin of Zoology. Raffles Bulletin of Zoology, 54.
Tanomtong, A. (2011). Cytogenetics. Khon Kaen University press.
Tanomtong, A., Supiwong, W., Jearranaiprepame, P., Khakhong, S., Kongpironchuen, C. & Getlekha, N. (2014). A new natural autotetraploid and chromosomal characteristics of dwarf snakehead fish, Channa gachua (Perciformes, Channidae) in Thailand. Cytologia, 79 (1), 15-27.
Vidthayanon, C. (2005). Freshwater Fish in Thailand (5th ed.). Nanmeebook Press.
Vinogradov, A.E. (2005). Genome size and chromatin condensation in vertebrates. Chromosoma, 113 (7), 362-369.
Wattanasirisareekul, R. & Suwannarak, A. (2012). Karyotype of peaceful betta, Betta imbellis (perciformes, Osphronemidae) from Thailand. Journal of Fisheries Technology Research, 2, 35-45.
Yan, H.Y. (1998). Auditory role of the suprabranchial chamber in gourami fish. Journal of Comparative Physiology A, 183, 325-333.
Downloads
Published
Issue
Section
License
Copyright (c) 2021 Science Technology and Engineering Journal (STEJ)
This work is licensed under a Creative Commons Attribution-NoDerivatives 4.0 International License.
