Chromosome study of Rasbora trilineata and Rasbora borapetensis (Cyprinidae, Cypriniformes): Reveal by conventional staining technique
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Abstract
The present study aims to construct the karyotype and idiogram of two Rasbora, namely Rasbora trilineata and R. borapetensis. Specimens were collected from Salween, Song Kharm, Mae Khlong basin and Sirindhorn Peat Swamp Forest, Thailand. Fish chromosome preparation was prepared directly from renal cells. Conventional staining technique was applied to stain the chromosomes with Giemsa’s solution. The results showed that two species the number of diploid chromosomes is 2n=50. The fundamental number (NF) of R. trilineata is 100, while R. borapetensis is 98. The types of chromosomes of R. trilineata were 12 metacentric, 24 submetacentric and 14 acrocentric chromosomes, and R. borapetensis eight metacentric 18 submetacentric, 20 acrocentric and four telocentric chromosomes. The karyotype formula are as follows: R. trilineata 2n (50) = 12m+24sm+14a, R. borapetensis 2n (50) = 8m+18sm+20a+4t.
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References
Donsakul, T. & Magtoon, W. (2002). Karyotype of Rasbora Caudimaculata, R. myersi, R. retrodorsalis, R. paviei from Thailand. Academic conference seminar for research papers of Srinakharinwirot University (pp. 1-7). Bangkok.
Donsakul, T. & Magtoon, W. (1995). Karyological studies of Puntius brevis (Bleeker, 1850) and Cyclocheilichthys enoplos in Thailand. Proceeding of 21st Congress on Science and Technology of Thailand, BO 14 (pp. 292-293).
Donsakul, T., Magtoon, W. & Rangsiruji, W. (2005). Karyotype of five species of fish (Pla siew) in subfamily Rasboranae. 31st Congress on Science and Technology of Thailand. Suranaree University of Technology. Nakhon Ratchasima Province Thailand.
Donsakul, T., Rangsiruji, A. & Magtoon, W. (2009). Karyotypes of five cyprinid fishes (Cyprinidae, DanioninaeDanionini): Rasbora agilis, R. dorsiocellata, R. rubrodorsalis, Boraras maculate and B. urophthalmoides from Thailand. In Proceedings of the 47th Kasetsart University Annual Conference (pp. 320-327). Kasetsart University, Thailand.
Eschmeyer, W.N. (2013). Catalog of Fishes electronic version. http:// research. calacademy.org/research/ ichthyology/catalog/fishcatmain.asp.
Froese, R. & Pauly, D. (2013). Fish Base, World Wide Web electronic publication. http:// www.fishbase. org.
Galetti, Jr. P.M., Molina, W.F., Affonso, P.R.A.M. & Aguilar, C.T. (2006). Assessing genetic diversity of Brazilian reef fishes by chromosomal and DNA markers. Genetica 126, 161-177.
Gornung, E., Cordisco, C., Rossi, A., Innocentiis, D.S., Crosetti, D. & Sola, L. (2001). Chromosomal evolution in Mugilidae: karyotype characterization of Liza saliens and comparative localization of major and minor ribosomal genes in the six Mediterranean mullets. Marine Biology, 139, 55-60.
Kavaco, K.F., Pazza, R., Bertollo, L.A.C. & Moreira-Filho, O. (2005). Molecular cytogenetics of Oligosarcus hepsetus (Teleotei, Chareciformes) from two Brazilian locations. Genetica , 124, 85-91.
Khuda-Bukhsh, A.R. (1979). Karyology of two species of hillstream fishes, Barilius bendelisis and Rasbora daniconius (Fam. Cyprinidae). Current Science, 48, 793-794.
Kottelat, M. (2001). Fishes of Laos. WHT Publications.
Kottelat, M. (1998). Fishes of the Nam Theun and Xe Bangfai basins, Laos, with diagnoses of twenty-two new species (Teleostei: Cyprinidae, Balitoridae, Cobitidae, Coiidae and Odontobutidae). Ichthyological Explorations of Freshwaters, 9(1), 1-128.
Kottelat, M. & Widjanarti, E. (2005). The fishes of Danau Sentarum National Park and the Kapuas Lakes area, Kalimantan Barat, Indonesia. Raffles Bulletin of Zoology Supplement, 13, 139-173.
Manna, G.K. & Khuda-Bukhsh, A.R. (1977). Karyomorphology of cyprinid fishes and cytological evaluation of the family. The Nucleus, 20, 119-127.
Monkolprasit, S., Sontirat, S., Vimollohakarn, S. & Songsirikul, T. (1997). Checklist of fishes in Thailand. Office of Environmental Policy and Planning.
Muchlisin, Z.A., Fadlia, N. & Siti Azizah, M.N. (2012). Genetic variation and taxonomy of Rasbora roup (Cyprinidae) from Lake Laut Tawar, Indonesia. Journal of Ichthyology, 52(4), 284-290.
Nirchio, M., Turner, B.J., Perez, J.E., Gaviria, J.I. & Cequea, H. (2002). Karyotypes of three species of toadfish (Batrachoididae: Teleostei) from Venezuela. Scientia Marina, 66, 1-4.
Phimphan, S., Chaiyasan, P., Suwannapoom, C., Reungsing, M., Juntaree, S., Tanomtong, A. & Supiwong W. (2020). Comparative karyotype study of three Cyprinids (Cyprinidae, Cyprininae) in Thailand by classical cytogenetic and FISH techniques. Comparative Cytogenetics, 14(4), 597-612.
Post, A. (1965). Vergleichede Untersuchungen der chromosomenzahlen bei Susswasser-Teleosteern. In: Gyldenholm, A.O. & Scheel, J.J. Chromosome number of fishes I. Journal Fish Biology, 3, 47-93.
Rainboth, W.J. (1996). Fishes of the Cambodian Mekong. FAO species identification field guide for fishery purposes. Rome.
Ren, X.H., Cui, J.X. & Yu, Q.X. (1996). Chromosomal nucleolar organizer regions differentiations in Chinese cyprinid fishes. Journal of Wuhan University (Natural Science Edition), 42, 475-480.
Seetapan, K. & Moeikum, T. (2004). Karyotypes of ten Cyprinid fishes (Family Cyprinidae). Journal Agricultural Extension, 22, 92-101.
Sola, L., De Innocentiis, S., Gornung, E., Papalia, S., Rossi, A.R., Marini, G., De Marco, P. & Cataudella, S. (2000). Cytogenetic analysis of Epinephelus marginatus (Pisces: Serranidae), with the chromosome localization of the 18S and 5S rRNA genes and of the (TTAGGG) on telomere sequence. Marine Biology, 137, 47-51.
Taki, Y. (1974). Fishes of the Lao Mekong Basin. United States Agency for International Development Mission to Laos Agriculture Division.
Thongnetr, W., Aiumsumang, S., Kongkaew, R., Tanomtong, A., Suwannapoom, C. & Phimphan, S. (2021). Cytogenetic characterisation and chromosomal mapping of microsatellite and telomeric repeats in two gecko species (Reptilia, Gekkonidae) from Thailand. Comp Cytogenet, 15(1), 41-52.
Turpin, R. & Lejeune, J. (1965). Les Chromosomes Humains. GauthierPillars.
Vidthayanon, C. (2002). Peat swamp fishes of Thailand. Office of Environmental Policy and Planning, Bangkok, Thailand.
Wang, S. Su, Y., Ding, S., Cai, Y. & Wang, J. (2010). Cytogenetic analysis of orange-spotted grouper, Epinephelus coioides, using chromosome banding and fluorescence in situ hybridization. Hydrobiologia, 638, 1-10.
Yeesaem, N., Jantarat, S. & Yeesin, P. (2019). Cytogenetic Characterigation of Rasbora einthovenii in Sirindhorn Peat Swamp Forest, Narathiwat Province. Journal of Fisheries Technology Research, 13, 58-68.