Chromosome study of Rasbora trilineata and Rasbora borapetensis (Cyprinidae, Cypriniformes): Reveal by conventional staining technique

Authors

  • Surachest Aiumsumang Biology Program, Faculty of Science and Technology, Phetchabun Rajabhat University, Phetchabun 67000, Thailand
  • Sumalee Phimphan Biology Program, Faculty of Science and Technology, Phetchabun Rajabhat University, Phetchabun 67000, Thailand
  • Alongklod Tanomtong Toxic Substances in Livestock and Aquatic Animals Research Group, Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen 40002, Thailand
  • Weerayuth Supiwong Faculty of Interdisciplinary Studies, Khon Kaen University, Nong Khai Campus, Muang, Nong Khai 43000, Thailand

Keywords:

Karyotype, Rasbora trilineata, Rasbora borapetensis

Abstract

The present study aims to construct the karyotype and idiogram of two Rasbora, namely Rasbora trilineata and R. borapetensis. Specimens were collected from Salween, Song Kharm, Mae Khlong basin and Sirindhorn Peat Swamp Forest, Thailand. Fish chromosome preparation was prepared directly from renal cells. Conventional staining technique was applied to stain the chromosomes with Giemsa’s solution. The results showed that two species the number of diploid chromosomes is 2n=50. The fundamental number (NF) of R. trilineata is 100, while R. borapetensis is 98. The types of chromosomes of R. trilineata were 12 metacentric, 24 submetacentric and 14 acrocentric chromosomes, and R. borapetensis eight metacentric 18 submetacentric, 20 acrocentric and four telocentric chromosomes. The karyotype formula are as follows: R. trilineata 2n (50) = 12m+24sm+14a, R. borapetensis 2n (50) = 8m+18sm+20a+4t.

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Published

22-11-2022 — Updated on 22-11-2022

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Vol. 8 No. 2 (2022): JULY-DECEMBER

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