องค์ประกอบทางเคมีของเปลือกถั่วดาวอินคา (PLUKENTIA VOLUBILIS L.) และการผลิตโอลิโกแซคคาไรด์ด้วยไซลาเนสทางการค้า (CHEMICALCOMPOSITION OF SACHA INCHI HULLS (PLUKENTIA VOLUBILIS L.) AND OLIGOSACCHARIDES PRODUCTION USING COMMERCIAL XYLANASE)
Keywords:
Sacha Inchi hulls, Oligosaccharides, Holocellulose, Xylanase, XylobioseAbstract
Sacha Inchi (PlukentiaVolubilis L.) hull is the biomass derived from the oil extraction, remaining fibers mainly hemicellulose and cellulose. The hemicelluloses were reported to be a good source of oligosaccharides production. The aim of this study was to analyze the chemical composition of SachaInchi hulls and study the effects of enzyme concentration and hydrolysis time of commercial endoxylanase, Pentopan Mono BG. Firstly, the chemical composition of Sacha Inchi hulls including holocellulose content, insoluble lignin, and extractives were analyzed. Then Sacha Inchi hulls were pretreated with alkali solution to remove the extractives,non-extractives and improve the enzymatic hydrolysis ability followed by enzymatic saccharification using commercial xylanase. The results showed that Sacha Inchi hulls consisted of protein(4.10%), fat(1.55%), crude fiber(37.20%), ash(1.44%), moisture(8.51%) and total carbohydrate(47.20%). Among those, the holocellulosewas found to be as much as 24.12%. To produce oligosaccharides, the alkali-pretreated biomass (2% NaOH, 6 days) was hydrolyzed by a commercial xylanase, Pentopan Mono BG (10-150 U/g, 50°C, pH 6.0 and 2-24 h). During hydrolysis with the enzyme content of 10 U/g, the total reducing sugar content was slightly increased while dramatically increased with that of 100, 133 and 150 U/g. The enzyme content of 133 U/g was found to be an optimum content as it gave total reducing sugar contents with no significant difference compared to 150 U/g. The TLC chromatogram was evident that xylose was absent during each incubation time interval and xylobiose was the main oligosaccharide having been produced since 4 h of incubation time. Hence, this study has proved the possibility of using Sacha Inchi hulls as an alternative source for the oligosaccharides production. And the oligosaccharide mixture produced in this study should be further investigated for the prebiotic properties.
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