• Jarupat Jundaeng Program in Clinical Dentistry, Faculty of Dentistry, Srinakharinwirot University
  • Teerachate Nantakeeratipat Department of Conservative Dentistry and Prosthodontics, Faculty of Dentistry, Srinakharinwirot University
  • Chuencheewit Thongsiri Department of Conservative Dentistry and Prosthodontics, Faculty of Dentistry, Srinakharinwirot University
  • Narongsak Laosrisin Department of Conservative Dentistry and Prosthodontics, Faculty of Dentistry, Srinakharinwirot University


Minocycline Gel, Periodontitis, Subgingival Ultrasonic Debridement, Type 2 Diabetes


Objective:To evaluated the clinical periodontal and inflammatory outcomes of adjunctive minocycline in patients with both periodontitis and type 2 diabetes mellitus (T2DM). Methods: Sixty patients with T2DM diagnosed with periodontitis were recruited and divided into control and test groups. All participants underwent a single-visit subgingival ultrasonic debridement, but only the test group received adjunctive sustained-release minocycline gel immediately afterward. Periodontal parameters, cytokines (IL-1b, IL-6, TNF-α and MCP-1), adiponectin, and Porphyromonas gingivalis (Pg) antibody were measured at baseline, 3 and 6 months after treatment. Results: Patients in both groups showed significant improvement in all clinical parameters after three and six months. The mean probing depth reduction and clinical attachment level gain after three months were significantly improved in the test group compared to the control group (p<0.05). IL-6, TNF-α, and MCP-1 were significantly reduced at the 3-month visit in both groups. Adiponectin was significantly increased at 6 months only in the test group. Moreover, Pg antibody was significantly reduced in both groups. Conclusion: Subgingival ultrasonic debridement combined with locally administered minocycline gel was effective in improving periodontal and inflammatory parameters in patients with T2DM at 3 months after treatment.


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Pihlstrom, B. L., Michalowicz, B. S., and Johnson, N. W. (2005, November 15). Periodontal diseases. Lancet, 366(9499), 1809-1820.

Stöhr, J., Barbaresko, J., Neuenschwander, M., and Schlesinger, S. (2021, July 1). Bidirectional association between periodontal disease and diabetes mellitus: A systematic review and meta-analysis of cohort studies. Sci Rep, 11(1), 1-9.

Stoicescu, M., Calniceanu, H., Tig, I., Nemeth, S., Tent, A., Popa, A., Brisc, C., and Ignat‑Romanul, I. (2021, July). Significant aspects and correlation between glycemic control and generalized chronic periodontitis in type 2 diabetes mellitus patients. Exp Ther Med, 22(1), 1-5.

Pan, W., Wang, Q., and Chen, Q. (2019, November 5). The cytokine network involved in the host immune response to periodontitis. Int J Oral Sci, 11(3), 1-13.

Radhakrishnan, P., Srikanth, P., Seshadri, K. G., Barani, R., and Samanta, M. (2014, July-August). Serum monocyte chemoattractant protein-1 is a biomarker in patients with diabetes and periodontitis. Indian J Endocrinol Metab, 18(4), 505.

Wang, Z., Chen, Z., Fang, F., and Qiu, W. (2021, May). The role of adiponectin in periodontitis: Current state and future prospects. Biomed Pharmacother, 137(1), 1-10.

Sun, W. L., Chen, L. L., Zhang, S. Z., Wu, Y. M., Ren, Y. Z., and Qin, G. M. (2011, August 1). Inflammatory cytokines, adiponectin, insulin resistance and metabolic control after periodontal intervention in patients with type 2 diabetes and chronic periodontitis. Intern Med, 50(15), 1569-1574.

O'Leary, T. J. (1986, February). The impact of research on scaling and root planing. J Periodontol, 57(2), 69-75.

Tunkel, J., Heinecke, A., and Flemmig, T. F. (2002). A systematic review of efficacy of machine‐driven and manual subgingival debridement in the treatment of chronic periodontitis. J Clin Periodontol, 29(3), 72-81.

Quirynen, M., Bollen, C. M. L., Vandekerckhove, B. N. A., Dekeyser, C., Papaioannou, W., and Eyssen, H. (1995, August). Full-vs. partial-mouth disinfection in the treatment of periodontal infections: short-term clinical and microbiological observations. J Dent Res, 74(8), 1459-1467.

Caffesse, R. G., Sweeney, P. L., and Smith, B. A. (1986, March). Scaling and root planing with and without periodontal flap surgery. J Clin Periodontol, 13(3), 205-210.

Greenstein, G., and Tonetti, M. (2000, January). The role of controlled drug delivery for periodontitis. The Research, Science and Therapy Committee of the American Academy of Periodontology. J Periodontol, 71(1), 125–140

Van Steenberghe, D., Rosling, B., Söder, P. Ö., Landry, R. G., Van der Velden, U., Timmerman, M. F. T., McCarthy, E. F., Vandenhoven, G., Wouters, C., Wilson, M., Matthews, J., and Newman, H. N. (1999, June 1). A 15‐month evaluation of the effects of repeated subgingival minocycline in chronic adult periodontitis. J Periodontol, 70(6), 657-667.

Lin, S. J., Tu, Y. K., Tsai, S. C., Lai, S. M., and Lu, H. K. (2012, April). Non-surgical periodontal therapy with and without subgingival minocycline administration in patients with poorly controlled type II diabetes: a randomized controlled clinical trial. Clin Oral Investig, 16(2), 599-609.

Munenaga, Y., Yamashina, T., Tanaka, J., Nishimura, F., and Hiroshima Study Group. (2013, April). Improvement of glycated hemoglobin in Japanese subjects with type 2 diabetes by resolution of periodontal inflammation using adjunct topical antibiotics: results from the Hiroshima Study. Diabetes Res Clin Pract, 100(1), 53-60.

Katagiri, S., Nitta, H., Nagasawa, T., Uchimura, I., Izumiyama, H., Inagaki, K., Kikuchi, T., and Noguchi, T. (2009, March). Multi-center intervention study on glycohemoglobin (HbA1c) and serum, high-sensitivity CRP (hs-CRP) after local anti-infectious periodontal treatment in type 2 diabetic patients with periodontal disease. Diabetes Res Clin Pract, 83(3), 308-315.

Silness, J., and Loe, H. (1964, February). Periodontal Disease in Pregnancy II. Correlation between Oral Hygiene and Periodontal Condition. Acta Odontologica Scandinavica, 22(1), 121-135.

Loe, H., and Silness, J. (1963, December). Periodontal disease in pregnancy I. Prevalence and severity. Acta Odontologica Scand, 21(6), 533-551.

Ainamo, J., and Bay, I. (1975, December). Problems and proposals for recording gingivitis and plaque. Int Dent J, 25(4), 229-235.

Yao, W., Xu, P., Pang, Z., Zhao, J., Chai, Z., Li, X., Li, H., Jiang, M., Cheng, H., Zhang, B., and Cheng, N. (2014, August 18). Local delivery of minocycline-loaded PEG-PLA nanoparticles for the enhanced treatment of periodontitis in dogs. Int J Nanomedicine, 9(1), 3963-3970.

Priyanka, N., Kalra, N., Saquib, S., Malgaonkar, N., Tarakji, B., Varsha, J., and Pradeep, A. R. (2015, September 30). Efficacy of Subgingivally Delivered Satranidazole in the Treatment of Type 2 Diabetes Subjects with Chronic Periodontitis: A Randomized Controlled Clinical Trial. J Int Acad Periodontol, 17(2), 42-48.

Agarwal, E., Bajaj, P., Naik, S. B., and Pradeep, A. R. (2017, December). Locally delivered 0.5% azithromycin as an adjunct to non‐surgical treatment in patients with chronic periodontitis with type 2 diabetes: a randomized controlled clinical trial. J Periodontol, 88(12), 1281-1287.

Jaubert de Beaujeu, M., Gignoux, M., and Mikaeloff, P. (1967). Results of surgical treatment of primary lung cancer. Review of statistics on 349 cases. Lyon Chir, 63(1), 520-535.

Heitz‐Mayfield, L. J. A., Trombelli, L., Heitz, F., Needleman, I., and Moles, D. (2002). A systematic review of the effect of surgical debridement vs. non‐surgical debridement for the treatment of chronic periodontitis. J Clin Periodontol, 29(3), 92-102.

Mailoa, J., Lin, G. H., Khoshkam, V., MacEachern, M., Chan, H. L., and Wang, H. L. (2015, October). Long‐term effect of four surgical periodontal therapies and one non‐surgical therapy: a systematic review and meta‐analysis. J Periodontol, 86(10), 1150-1158.

Aziz, A., Kalekar, M., Suryakar, A., Kale, R., Benjamin, T., and Dikshit, M. (2018, August 1). Pre-and post-treatment effectiveness of SRP on levels of IL-6, IL-10, and CRP in chronic periodontitis patients with and without diabetes. Am J Biochem, 8(1), 1-6.

Taba, M., Kinney, J., Kim, A. S., and Giannobile, W. V. (2005, July). Diagnostic biomarkers for oral and periodontal diseases. Dent Clin North AM, 49(3), 551-571.

Kardesler, L., Buduneli, N., Çetinkalp, S., and Kinane, D. F. (2010, January). Adipokines and inflammatory mediators after initial periodontal treatment in patients with type 2 diabetes and chronic periodontitis. J Periodontol, 81(1), 24-33.

Yooyen, J., Srisuwantha, R., Nantakeeratipat, T., Thongsiri, C., and Laosrisin, N. (2022, January-June). Change of hemoglobin A1C fasting plasma and hS-CRP levels after one visit subgingival ultrasonic debridement with adjunctive minocycline gel in type II diabetis-periodontitis patients. Srinakharinwirot university (journal of science and technology), 14(27), 24-36.

Talbert, J., Elter, J., Jared, H. L., Offenbacher, S., Southerland, J., and Wilder, R. S. (2006, April 1). The effect of periodontal therapy on TNF-α, IL-6 and metabolic control in type 2 diabetics. J Dent Hyg, 80(2), 7.

Arakelyan, A., Petrkova, J., Hermanova, Z., Boyajyan, A., Lukl, J., and Petrek, M. (2005, August 14). Serum levels of the MCP-1 chemokine in patients with ischemic stroke and myocardial infarction. Mediators Inflamm, 2005(3), 175-179.

Kiyici, S., Erturk, E., Budak, F., Ersoy, C., Tuncel, E., Duran, C., Oral, B., Sigirci, D., and Imamoglu, S. (2006, November). Serum monocyte chemoattractant protein-1 and monocyte adhesion molecules in type 1 diabetic patients with nephropathy. Arch Med Res, 37(8), 998-1003.

Hajishengallis, G., Darveau, R. P., and Curtis, M. A. (2012, October). The keystone-pathogen hypothesis. Nat Rev Microbiol, 10(10), 717-725.

Morozumi, T., Yashima, A., Gomi, K., Ujiie, Y., Izumi, Y., Akizuki, T., Mizutani, K., Takamatsu, H., Minabe, M., Miyauchi, S., Yoshino, T., Tanaka, M., Tanaka, Y., Hokari, T., and Yoshie, H. (2018, August). Increased systemic levels of inflammatory mediators following one‐stage full‐mouth scaling and root planing. J Periodontal Res, 53(4), 536-544.

Soeroso, Y., Akase, T., Sunarto, H., Kemal, Y., Salim, R., Octavia, M., Viandita, A., Setiawan, J., and Bachtiar, B. M. (2017, March 10). The risk reduction of recurrent periodontal pathogens of local application minocycline HCl 2% gel, used as an adjunct to scaling and root planing for chronic periodontitis treatment. Ther Clin Risk Manag, 13(1), 307-3014.

Silva, T. E., Colombo, G., and Schiavon, L. L. (2014, April). Adiponectin: A multitasking player in the field of liver diseases. Diabetes Metab, 40(2), 95-107.

Keller, P., Møller, K., Krabbe, K. S., and Pedersen, B. K. (2003, October). Circulating adiponectin levels during human endotoxaemia. Clin Exp Immunol, 134(1), 107-110.

Maeda, N., Shimomura, I., Kishida, K., Nishizawa, H., Matsuda, M., Nagaretani, H., Furuyama, N., Kondo, H., Takahashi, M., Arita, Y., Komuro, R., Ouchi, N., Kihara, S., Tochino, Y., Okutomi, K., Horie, M., Takeda, S., Aoyama, T., Funahashi, T., and Matsuzawa, Y. (2002, July). Diet-induced insulin resistance in mice lacking adiponectin/ACRP30. Nat Med, 8(7), 731-737.

Yokota, T., Oritani, K., Takahashi, I., Ishikawa, J., Matsuyama, A., Ouchi, N., Kihara, S., Funahashi, T., Tenner, A. J., Tomiyama, Y., and Matsuzawa, Y. (2000, September 1). Adiponectin, a new member of the family of soluble defense collagens, negatively regulates the growth of myelomonocytic progenitors and the functions of macrophages. Blood, 96(5), 1723-1732.

Kappes, A., and Loeffler, G. (2000, November-December). Influences of ionomycin, dibutyryl-cycloAMP and tumour necrosis factor-alpha on intracellular amount and secretion of apM1 in differentiating primary human preadipocytes. Horm Metab Res, 32(11/12), 548-554.

Boström, L., Linder, L. E., and Bergström, J. (1998, October). Clinical expression of TNF‐α in smoking‐associated periodontal disease. J Clin Periodontol, 25(10), 767-773.

Jain, P., Ved, A., Dubey, R., Singh, N., Parihar, A. S., and Maytreyee, R. (2020, October-December). Comparative evaluation of serum tumor necrosis factor α in health and chronic periodontitis: A case–control study. Contemp Clin Dent, 11(4), 342-349.

Gopinath, V., Ramakrishnan, T., Emmadi, P., Ambalavanan, N., and Mammen, B. (2009, May). Effect of a controlled release device containing minocycline microspheres on the treatment of chronic periodontitis: A comparative study. J Indian Soc Periodontol, 13(2), 79-84.

Van Steenberghe, D., Bercy, P., Kohl, J., De Boever, J., Adriaens, P., Vanderfaeillie, A., Adriaenssen, C., Rompem, E., Vree, H. D., McCarthy, E. F., and Vandenhoven, G. (1993, July). Subgingival minocycline hydrochloride ointment in moderate to severe chronic adult periodontitis: a randomized, double‐blind, vehicle‐controlled, multicenter study. J Periodontol, 64(7), 637-644.




How to Cite

Jundaeng, J. ., Nantakeeratipat, T. ., Thongsiri, C., & Laosrisin, N. . (2022). EFFECTS OF SINGLE-VISIT SUBGINGIVAL ULTRASONIC DEBRIDEMENT WITH ADJUNCTIVE MINOCYCLINE GEL ON PERIODONTAL AND INFLAMMATORY PARAMETERS IN PATIENTS WITH TYPE 2 DIABETES. Srinakharinwirot University Journal of Sciences and Technology, 14(28, July-December), 36–52. Retrieved from https://ph02.tci-thaijo.org/index.php/swujournal/article/view/248077