COMPARISON OF PROTEIN CONTENT AND ANTIOXIDANT ACTIVITY IN BREAST MILK BASED ON PATHOMCHINDA SCRIPTURE
Keywords:Traditional Thai Medicine, Pathomchinda Scripture, Breast Milk, Protein, Antioxidant Activity
High quality of breast milk promotes the immune system and healthy growth of newborn infants. This study aims to proof the quality of milk. The first objective is to compare the protein content and the antioxidant activity of breast milk between good and bad character of mother in Pathomchinda scripture. Second objective is to evaluate the relationship between obstetrical factors, namely maternal age, gestational age, birth weight, underline disease, maternal complication, delivery method and lactogenesis phase, and the protein content and antioxidant activity of breast milk. The subject consisted of 30 breastfeeding mothers for 4 days – 6 months located in Songkhla province. The study adopted purposive sampling technique. The selection of subject was done by snowball sampling technique. According to Pathomchinda scripture, the subjects were divided into 2 groups: good character and bad character, by using the personal characteristics assessment between January and April 2020. The researchers collected 15 ml of milk samples using freeze-drying technique before determine protein content and antioxidant activity by Bradford and DPPH methods, respectively. Characters of population were described by descriptive statistic. Data were analyzed by Mann-Whitney test and Spearman’s rank correlation coefficients. The results showed that the protein content from good character mother (mean ± S.D. = 1.04 ± 0.23 mmol/L) and the protein content from bad character mother (mean ± S.D. = 0.91 ± 0.22 mmol/L) was not significant with p > 0.05. However, the antioxidant activity from good character mother (mean = 56.75, S.D. = 1.67) provided significance higher percent inhibition of free radicals than the milk from bad character mother (mean = 47.35, S.D. = 3.24) with p < 0.05. Therefore, the quality of milk from good character group is better than another group related to the Pathomchinda scripture. In addition, lactogenesis phase was found to be the only factor associated with protein content and antioxidant activity in breast milk. Maternal age and pregnancy complications factors were associated with antioxidant activity. However, the composition of human breast milk is a complex combination of lipids, carbohydrates, and minerals, which are essential for infant nutrition. Human breast milk also contains lots of distinct bioactive molecules that indicate the quality of milk. Hence, the relationship between the individual maternal characteristics and other nutrients in breast milk should be further studied.
Foundation of Thai Traditional Medicine. (2016). Original Thai medical textbooks: conservation book. (4th ed.) Bangkok: Supawanit Publishing House.
Andreas, N. J., Kampmann, B., and Mehring Le-Doare, K. (2015). Human breast milk: a review on its composition and bioactivity. Early human development, 91(11), 629-635.
Arianna, A., Isadora, B., Silvia, M., Giacomo, F., and Luigi, C. (2018). Oxidative stress and necrotizing enterocolitis: pathogenetic mechanisms, opportunities for Intervention, and role of human milk. Oxidative Medicine and Cellular Longevity, 2018, 1-7.
Matos, C., Ribeiro, M., and Guerra, A. (2015). Breastfeeding: antioxidative properties of breast milk. Journal of applied biomedicine, 13(3), 169-180.
Bachour, P., Yafawi, R., Jaber, F., Choueiri, E., and Abdel-Razzak, Z. (2012). Effects of smoking, mother's age, body mass index, and parity number on lipid, protein, and secretory immunoglobulin A concentrations of human milk. Breastfeeding medicine: the official journal of
the Academy of Breastfeeding Medicine, 7(3), 179-88.
Butts, C. A., Hedderley, D. I., Herath, T. D., Paturi, G., Glyn-Jones, S., Wiens, F., Stahl, B., and Gopal, P. (2018). Human milk composition and dietary intakes of breastfeeding women of different ethnicity from the Manawatu-Wanganui Region of New Zealand. Nutrients, 10(9), 1231.
Nouroozi, R.V., Noroozi, M.V., and Ahmadizadeh, M. (2015). Determination of protein concentration using bradford microplate protein quantification assay. International Electronic Journal of Medicine, 4(1), 11-17.
Martysiak-zurowska, D., and Wenta, W. (2012). A comparison of ABTS and DPPH methods for assessing the total antioxidant capacity of human milk. Acta scientiarum polonorum. Technologia alimentaria, 11(1), 83-89.
Mehta, R., and Petrova, A. (2014). Is variation in total antioxidant capacity of human milk associated with levels of bio-active proteins? Journal of Perinatology, 34, 220-222.
Mei-Yung, and Petrova A. (2014). Factor affecting human milk composition. Pediatrics and Neonatology, 55, 421-422.
Jelena, Z., Slavica, S., Natasa, T., Marko, D., Gordana, K., and Tatjana, J. (2015). Antioxidants and antioxidant capacity of human milk. Acta Facultatis Medicae Naissensis, 32(2), 115-125.
Tijerina-Saenz, A., Innis, S. M., and Kitts, D. D. (2009). Antioxidant capacity of human milk and its association with vitamins A and E and fatty acid composition. Acta paediatrica, 98(11), 1793-1798.
Gidrewicz, D. A., and Fenton, T. R. (2014). A systematic review and meta-analysis of the nutrient content of preterm and term breast milk. BMC pediatrics, 14, 216.
Zarban, A., Taheri, F., Chahkandi, T., Sharifzadeh, G., and Khorashadizadeh, M. (2009). Antioxidant and radical scavenging activity of human colostrum, transitional and mature milk. Journal of clinical biochemistry and nutrition, 45(2), 150-154.
Ballard, O., and Morrow, A. L. (2013). Human milk composition: nutrients and bioactive factors. Pediatric clinics of North America, 60(1), 49-74.
Klein, K., Bancher-Todesca, D., Graf, T., Garo, F., Roth, E., Kautzky-Willer, A., and Worda, C. (2013). Concentration of free amino acids in human milk of women with gestational diabetes mellitus and healthy women. Breastfeeding medicine: the official journal of the Academy of
Breastfeeding Medicine, 8(1), 111-115.
Morceli, G., Honorio-França, A. C., Fagundes, D. L., Calderon, I. M., and França, E. L. (2013). Antioxidant effect of melatonin on the functional activity of colostral phagocytes in diabetic women. PloS one. 8(2), Article e56915.
Paduraru, L., Dimitriu, D.C., Avasiloaiei, A.L., Moscalu, M., Zonda, G.I., and Stamatin, M. (2018). Total antioxidant status in fresh and stored human milk from mothers of term and preterm neonates. Pediatrics & Neonatology, 59(6), 600-605.
Olivares, M., Albrecht, S., De Palma, G., Ferrer, M. D., Castillejo, G., Schols, H. A., and Sanz, Y. (2015). Human milk composition differs in healthy mothers and mothers with celiac disease. European journal of nutrition, 54(1), 119-128.
Nuampa, S., Sinsuksai, N., Phahuwatanakorn, W., and Chanprapaph, P. (2014). Personal factors, first feeding time and nurse support in predicting successful exclusive breastfeeding at discharge in mothers with cesarean section. Journal of Nursing Science, 31(2), 49-59.
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