PRELIMINARILY STUDY OF TOUCH DNA DETECTION AND BUCCAL ON COTTON SWAB BY USING SYBR®GREEN I NUCLEIC ACID GEL STAIN
Keywords:
Touch DNA, SYBR Green I, Cotton swab, Forensic ScienceAbstract
Touch DNA and buccal samples are among the most common sample types submitted to DNA forensic laboratory. However, some Touch DNA samples and buccal collections don’t realize DNA appears on the sample until the DNA detection processing is completed. This study aims to investigate the touch DNA and buccal sample detection on cotton swabs by using SYBR Green I Nucleic Acid Gel Stain. So, this screening method reduces samples and resources in Thailand DNA forensic laboratory processing. Touch DNA samples were collected from male and female volunteers. Buccal samples were collected from female volunteers. All samples were stained with 5x SYBR®Green I and detected using Gel Documentation System (Major Science, USA) and Fluorescence microscopy. Fluorescence levels were detected by Image J compared with a non-DNA cotton swab. The results showed that Cotton swabs are unsuitable for the touch DNA screening method However it can be used to detect DNA from Buccal swabs. Cotton swab that collect Buccal sample and stain it with 5X SYBR®Green I revealed that SYBR green I fluorescence was visualized significant differently from DNA-free swab at 95% confidence interval when tested with Paired Samples T-Test (p-value > 0.05).
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