• Thitima Parkpoom Department of Environmental Science, Faculty of Science, Khon Kaen University.
  • Parinda Suksabye Department of Urban and Industrial Environment, Faculty of Science and Technology, Suan Dusit University.
  • Phot Parkpoom Department of Environmental Health Occupational Health and Safety, Faculty of Public Health, Khon Kaen University.
  • Kanlayaporn Chantree Department of Cosmetic and Beauty Sciences Program, Faculty of Science and Technology, Kanchanaburi Rajabhat University.
  • Sureeporn Thummamikkapong Department of Environmental Science, Faculty of Science and Technology, Rajabhat Rajanagarindra University.


Antioxidant, Cytotoxic, Cyperus Rotundus, Kaempferia Parviflora


The purpose of this study was to analyze the total phenolic, total flavonoids, antioxidant, cytotoxic activity and antioxidant components of crude extracts from eight species of Thai local plants using different solvents such as ethanol and hexane. Ethanol showed significantly higher percentage extraction yields than hexane. The contents of total phenolic and total flavonoids in the crude ethanol extract of Cyperus rotundus (CRE) were the highest at 11.617±0.289 mg GAE/g dry extract and 7.973±0.231 mg QE/g dry extract, respectively. The free radical scavenging of crude ethanol extracts of C. rotundus was the highest compared to the other plants and showed IC50 values in the DPPH assay and ABTS assay of 0.408±0.011 mg/ ml and 0.402±0.0071 mg/ml, respectively. Moreover, crude ethanol extracts of C. rotundus indicated the presence of catechin (m/z 289.1) and anthocyanin (m/z 463.2), as analyzed by the LC-MS method. The catechin content was important in corroborating antioxidant activity. Studies of the in vitro cytotoxic activity of the crude ethanol extracts of C. rotundus against the NCI-H187 cell line showed IC50 values of 42.47±2.66 µg/ml. In addition, the crude ethanol extract of Kaempferia parviflora against KB and NCI-H 187 cell lines showed IC50 values of 60.24±1.73 and 27.22±1.04 µg/ml, respectively. These results indicated that the traditional medicinal plants C. rotundus and K. parviflora are excellent sources of antioxidants and potential cytotoxic agents against KB and NCI-H 187 cell lines.


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Palipoch, S., Punsawad, C., and Suwannalert, P. (2013). Thunbergia laurifolia, a new choice of natural antioxidant to prevent oxidative stress-related pathology: A review. Journal of Medicinal Plants Research, 7(12), 698-701.

Al-Owaisi, M., Al-Hadiwi, N., and Khan, S. A. (2014). GC-MS analysis, determination of total phenolics, flavonoid content free radical scavenging activities of various crude extracts of Moringa peregrina (Forssk.) Fiori leaves. Asian Pacific Journal of Tropical Biomedicine, 4(12), 964-970.

Tantengco, O. A. G., and Jacinto, S. D. (2015). Cytotoxic activity of crude extracts fractions from Premna odorata (Blanco), Artocarpus camansi (Blanco) Gliricidia sepium (Jacq.) against selected human cancer cell lines. Asian Pacific Journal of Tropical Biomedicine, 5(12), 1037-1041.

Pitt, J. J. (2009). Principles applications of liquid chromatography-mass spectrometry in clinical biochemistry. The Clinical Biochemist Reviews, 30(1), 19-34.

Figueroa, L. A., Navarro, L. B., Vera, M. P., and Petricevich, V. L. (2014). Antioxidant activity, total phenolic flavonoid contents, cytotoxicity evaluation of Bougainvillea xbuttiana. International Journal of Pharmacy Pharmaceutical Sciences, 6(5), 497-502.

Br-Williams, W., Cuvelier, M. E., and Berset, C. (1995). Antioxidative activity of phenolic composition of commercial extracts of sage rosemary. Lwt, 28, 25-30.

O'brien, J., Wilson, I., Orton, T., and Pognan, F. (2000). Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. European Journal of Biochemistry, 267(17), 5421-5426.

Bao, J., Cai, Y., Sun, M., Wang, G., and Corke, H. (2005). Anthocyanins, flavanols, free radical scavenging activity of Chinese bayberry (Myrica rubra) extracts their color properties stability. Journal of Agricultural Food Chemistry, 53(6), 2327-2332.

Hunt, L., Jordan, M., De Jesus, M., and Wurm, F. M. (1999). GFP‐expressing mammalian cells for fast, sensitive, noninvasive cell growth assessment in a kinetic mode. Biotechnology Bioengineering, 65(2), 201-205.

Boonsong, P., Laohakunjit, N., and Kerdchoechuen, O. (2012). Natural pigments from six species of Thai plants extracted by water for hair dyeing product application. Journal of Cleaner Production, 37, 93-106.

Sriket, P. (2014). Chemical components and antioxidant activities of Thai local vegetables. KMITL Science and Technology Journal, 14(1), 18-23.

Tian, L., Zhao, Y., Guo, C., and Yang, X. (2011). A comparative study on the antioxidant activities of an acidic polysaccharide various solvent extracts derived from herbal Houttuynia cordata. Carbohydrate Polymers, 83(2), 537-544.

Settharaksa, S., Jongjareonrak, A., Hmadhlu, P., Chansuwan, W., and Siripongvutikorn, S. (2012). Flavonoid, phenolic contents antioxidant properties of Thai hot curry paste extract its ingredients as affected of pH, solvent types high temperature. International Food Research Journal, 19(4), 1581-1587.

Chen, J., Yang, J., Ma, L., Li, J., Shahzad, N., and Kim, C. K. (2020). Structure-antioxidant activity relationship of methoxy, phenolic hydroxyl, carboxylic acid groups of phenolic acids. Scientific Reports, 10(1), 1-9.

De Bruijn, J., Loyola, C., Aqueveque, P., Cañumir, J., Cortéz, M., and France, A. (2009). Antioxidant properties of extracts obtained from Grifola gargal mushrooms. Micologia Aplicada International, 21(1), 11-18.

Samariya, K., and Sarin, R. (2013). Isolation identification of flavonoids from Cyperus rotundus Linn. in vivo in vitro. Journal of Drug Delivery Therapeutics, 3(2), 109-113.

Vichitphan, S., Vichitphan, K., and Sirikhansaeng, P. (2007). Flavonoid content and antioxidant activity of krachai-dum (Kaempferia parviflora) wine. KMITL Science and Technology Journal, 7(2-1), 97-105.

Tachakittirungrod, S., Okonogi, S., and Chowwanapoonpohn, S. (2007). Study on antioxidant activity of certain plants in Thai: Mechanism of antioxidant action of guava leaf extract. Food Chemistry, 103(2), 381-388.

Priya, R., Prathapan, A., Raghu, K. G., and Menon, A. N. (2012). Chemical composition in vitro antioxidative potential of essential oil isolated from Curcuma longa L. leaves. Asian Pacific Journal of Tropical Biomedicine, 2(2), S695-S699.

Kamala, A., Middha, S. K., Gopinath, C., Sindhura, H. S., and Karigar, C. S. (2018). In vitro antioxidant potentials of Cyperus rotundus L. rhizome extracts their phytochemical analysis. Pharmacognosy Magazine, 14(54), 261-267.

Wanich, S., and Yenjai, C. (2009). Amino nitro derivatives of 5, 7-dimethoxyflavone from Kaempferia parviflora cytotoxicity against KB cell line. Archives of Pharmacal Research, 32(9), 1185-1189.

Hossain, M., Wongsrikaew, N., Yoo, G. W., Han, J., and Shin, C. G. (2012). Cytotoxic effects of polymethoxyflavones isolated from Kaempferia parviflora. Journal of the Korean Society for Applied Biological Chemistry, 55(4), 471-476.

Yenjai, C., Prasanphen, K., Daodee, S., Wongpanich, V., and Kittakoop, P. (2004). Bioactive flavonoids from Kaempferia parviflora. Fitoterapia, 75(1), 89-92.

Wangthong, S., Palaga, T., Rengpipat, S., Wanichwecharungruang, S. P., Chanchaisak, P., and Heinrich, M. (2010). Biological activities safety of Thanaka (Hesperethusa crenulata) stem bark. Journal of Ethnopharmacology, 132(2), 466-472.

Mongelli, E., Desmarchelier, C., Talou, J. R., Coussio, J., and Ciccia, G. (1997). In vitro antioxidant cytotoxic activity of extracts of Baccharis coridifolia DC. Journal of ethnopharmacology, 58(3), 157-163.




How to Cite

Parkpoom, T., Suksabye, P., Parkpoom, P., Chantree, K., & Thummamikkapong, S. (2024). ANTIOXIDANT AND CYTOTOXIC ACTIVITIES OF THAI LOCAL PLANT EXTRACTS. Srinakharinwirot University Journal of Sciences and Technology, 16(31, January-June), 1–16, Article 253652. Retrieved from