Assessing the soil bacterial community and minerals around roots of tiger grass (Thysanolaena latifolia) from Mae Sot District, Tak Province, Thailand

Authors

  • Kanittha Bangpra Faculty of Environmental Culture and Eco-tourism, Srinakharinwirot University.
  • Wirongrong Duangjai Department of Silviculture, Faculty of Forestry, Kasetsart University.
  • Phruet Racharak Department of Forest Biology, Faculty of Forestry, Kasetsart University.
  • Patarapong Kroeksakul Faculty of Environmental Culture and Eco-tourism, Srinakharinwirot University.
  • Phanom Sutthisaksopon Faculty of Environmental Culture and Eco-tourism, Srinakharinwirot University.
  • Jutatip Junead Faculty of Environmental Culture and Eco-tourism, Srinakharinwirot University.
  • Praepilai Mittrarath Faculty of Environmental Culture and Eco-tourism, Srinakharinwirot University.
  • Arin Ngamniyom Faculty of Environmental Culture and Eco-tourism, Srinakharinwirot University.

Keywords:

V3-4, NGS, Microbes, Highland, Tiger Grass

Abstract

This study aimed to investigate the soil bacterial community and minerals near the roots of tiger grass (Thysanolaena latifolia) from Mae Sot District, Tak Province. Soil samples were collected from three sites. Site 1 was an area that had been cultivated for maize. Site 2 had never been used for agriculture. Site 3 had been cultivated for cabbage. For the microbiota analysis, a HiSeq 2500 system was used to perform Illumina next-generation sequencing to obtain the bacterial sequences targeted to V3-V4 of the 16S rRNA gene. Moreover, pH, soil moisture, temperature, electrical conductivity, the C/N ratio, and the contents of potassium (K), phosphorus (P), sulfur (S), aluminium (Al), manganese (Mn), nickel (Ni), copper (Cu), iron (Fe) and zinc (Zn) were evaluated. The operational taxonomic units (OTUs) of the microbes at sites 1, 2 and 3 were 4,226, 3,937 and 2,958, respectively. The number of OTUs shared by all the samples and sites was 1,684. In phyla, "Proteobacteria" was the most abundant at sites 2 and 3. In contrast, "Acidobacteriota", "Planctomycetota" and "Verrucomicrobiota" were most abundant at site 1, but "Candidatus Thermoplasmatota" was predominant at site 2. In terms of genera, Methylobacillus and Pseudarthrobacter were the most abundant at site 2, but Sphingomonas was predominant at site 3. The values for pH, soil moisture, the C/N ratio, and the contents of K, Ni, Cu, Fe and Zn were significantly different among the three sites. These results may provide important data to support an understanding of soil microbes and minerals in waste lands. The results revealed that the core bacterial elements may depend on the characteristics of the soil and environment. In addition, these data may help support the restoration of land in the future.

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Published

2025-06-01

How to Cite

Bangpra, K., Duangjai, W., Racharak, P., Kroeksakul, P., Sutthisaksopon, P., Junead, J., Mittrarath, P., & Ngamniyom, A. (2025). Assessing the soil bacterial community and minerals around roots of tiger grass (Thysanolaena latifolia) from Mae Sot District, Tak Province, Thailand. Srinakharinwirot University Journal of Sciences and Technology, 17(1, January-June), 1–13, Article 255785. retrieved from https://ph02.tci-thaijo.org/index.php/swujournal/article/view/255785

Issue

Vol. 17 No. 1, January-June (2025): Srinakharinwirot University Journal of Sciences and Technology

Section

บทความวิจัย

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