DETERMINATION OF PESTICIDE RESIDUES IN CITRUS RETICULATA USING GC-MS/MS WITH ETHYL ACETATE EXTRACTION
Keywords:
Citrus Reticulata, Pesticide Residues, Ethyl Acetate, GC-MS/MSAbstract
A simple, fast, inexpensive, and sensitive method, namely ethyl acetate extraction was developed for analysis of pesticide residues in Citrus Reticulata. The optimum conditions for extraction of pesticide residues were orange sample (g): sodium hydrogen carbonate (g): sodium sulphate (g): ethyl acetate (ml) in the ratio of 10:3: 10:20. Eleven types of pesticide residues in Citrus Reticulata were analyzed by using gas chromatography tandem mass spectrometry (GC-MS/MS). The linear calibration curve was in the range of 0.005 - 1.000 mg/kg-1 with a correlation coefficient (R2) higher than 0.9950. The percentage recovery and relative standard deviations (%RSD) were in the range of 68.38 – 108.16 and 2.51 – 19.19, respectively. The limit of detection of the method was 0.50 µg/kg-1 and limit of quantification was 1.70 µg/kg-1. From the analysis of 50 citrus samples, it was found that there were pesticide residues in 43 samples, and the amount exceeded the Agricultural Product Certification Standards in 5 samples. The ethyl acetate extraction followed by GC-MS/MS determination were suitable method for the analysis of pesticide residues in Citrus Reticulata samples.
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