DETERMINATION OF PESTICIDE RESIDUES IN CITRUS RETICULATA USING GC-MS/MS WITH ETHYL ACETATE EXTRACTION

Authors

  • Sirirat Phaisansuthichol Program in Chemistry, Faculty of Science, Maejo University.
  • Sureewan Saengsrichan Program in Chemistry, Faculty of Science, Maejo University.
  • Nongpanga Olsen Office of Agricultural Research and Development Region 1, Department of Agriculture.

Keywords:

Citrus Reticulata, Pesticide Residues, Ethyl Acetate, GC-MS/MS

Abstract

A simple, fast, inexpensive, and sensitive method, namely ethyl acetate extraction was developed for analysis of pesticide residues in Citrus Reticulata. The optimum conditions for extraction of pesticide residues were orange sample (g): sodium hydrogen carbonate (g): sodium sulphate (g): ethyl acetate (ml) in the ratio of 10:3: 10:20. Eleven types of pesticide residues in Citrus Reticulata were analyzed by using gas chromatography tandem mass spectrometry (GC-MS/MS). The linear calibration curve was in the range of 0.005 - 1.000 mg/kg-1 with a correlation coefficient (R2) higher than 0.9950. The percentage recovery and relative standard deviations (%RSD) were in the range of 68.38 – 108.16 and 2.51 – 19.19, respectively. The limit of detection of the method was 0.50 µg/kg-1 and limit of quantification was 1.70 µg/kg-1. From the analysis of 50 citrus samples, it was found that there were pesticide residues in 43 samples, and the amount exceeded the Agricultural Product Certification Standards in 5 samples. The ethyl acetate extraction followed by GC-MS/MS determination were suitable method for the analysis of pesticide residues in Citrus Reticulata samples.

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Published

2024-10-07

How to Cite

Phaisansuthichol, S., Saengsrichan, S., & Olsen, N. (2024). DETERMINATION OF PESTICIDE RESIDUES IN CITRUS RETICULATA USING GC-MS/MS WITH ETHYL ACETATE EXTRACTION . Srinakharinwirot University Journal of Sciences and Technology, 16(32, July-December), 1–12, Article 256027. Retrieved from https://ph02.tci-thaijo.org/index.php/swujournal/article/view/256027

Issue

Vol. 16 No. 32, July-December (2024): Srinakharinwirot University Journal of Sciences and Technology

Section

บทความวิจัย

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