Evaluation of primary probiotic properties of bacteriocin-producing lactic acid bacteria isolated from Southern Thailand fermented foods

Authors

  • Sirinat Srionnual Department of Food and Service Management, Faculty of Agro-Industy, Rajamangala University of Technology Srivijaya.
  • Nomchit Kaewthai Andre Department of Food and Service Management, Faculty of Agro-Industy, Rajamangala University of Technology Srivijaya.
  • Saowanee Chaipetch Department of Food and Service Management, Faculty of Agro-Industy, Rajamangala University of Technology Srivijaya.
  • Tanikarn Torasin Department of Food and Service Management, Faculty of Agro-Industy, Rajamangala University of Technology Srivijaya.

Keywords:

Probiotic, Lactic Acid Bacteria, Southern Thailand Fermented Foods

Abstract

The 6 strains (PPD90 PS113 KS173 HSD392 HSD398 and PPD444) of bacteriocin-producing lactic acid bacteria isolated from southern Thailand fermented foods were evaluated their primary probiotic properties. Among the selected strains, PS113 showed the best probiotic potential with survival at pH 3.0 (84.02%), high tolerance to acid followed by 0.3% (w/v) bile salt, high tolerance to bile salt, survived well in aerobic and anaerobic conditions and resistant to penicillin and tetracycline, as following by PPD90 KS173 and HSD392 because the different of their survive in test conditions. By analysis of their 16S rDNA gene sequences, PPD90 was identified as Lactobacillus pentosus with 99.15% similarity, PS113 was identified as Lactobacillus plantarum with 100% similarity, KS173 was identified as Lactobacillus pentosus with 100% similarity and HSD392 was identified as Enterococcus faecium with 99.79% similarity.

Downloads

Download data is not yet available.

References

Chanchaichaovivat, A., Panvilai, S., Prajanban, J., and Phornphisutthimas, S. (2020). Important pathogenic microorganisms causing intestinal diseases. Journal of Research Unit on Science, Technology and Environment for Learning, 11(1), 188-206. (in Thai)

Gueimonde, M., Frias, R., and Ouwehand, A. C. (2006). Assuring the continued safety of lactic acid bacteria used as probiotics. Biologia, 61(6), 755-760. https://doi.org/10.2478/s11756-006-0153-2

La-ongkham, O. (2019). Probiotics: The selected microorganism for human health. Food Journal, 49(4), 29-38. (in Thai)

Dicks, L. M. T., and Botes, M. (2010). Probiotic lactic acid bacteria in the gastro-intestinal tract: Health benefits, safety and mode of action. Beneficial Microbes, 1(1), 11-29.

Quinto, E. J., Jiménez, P., Caro, I., Tejero, J., Mateo, J., and Girbés, T. (2014). Probiotic lactic acid bacteria: A review. Food and Nutrition Sciences, 5(18), 1765-1775.

Kuasuwan, P., Charernjiratrakul, W., and Kantachote, D. (2014). Selection of probiotic lactic acid bacteria to be used as starter culture for pickles production. In L., Manmart, U., Tattawasart and D. Ayuwat (Eds.), Graduate Research Conference 2014 (pp. 667-676). Khon Kaen University. (in Thai)

Pennacchia, C., Vaughan, E. E., and Villani, F. (2006). Potential probiotic Lactobacillus strains from fermented sausages: Further investigations on their probiotic properties. Meat Science, 73(1), 90-101.

Hoque, M. Z., Akter, F., Hossain, K. M., Rahman, M. S. M., Billah, M. M., and Islam, K. M. D. (2010). Isolation, identification and analysis of probiotic properties of Lactobacillus spp. from selective regional yoghurt. World Journal of Dairy and Food Sciences, 5(1), 39-46.

Kim, Y. G., Choi, S-I., Jeong, Y., and Kang, C-H. (2022). Evaluation of safety and probiotic potential of Enterococcus faecalis MG5206 and Enterococcus faecium MG5232 isolated from kimchi, a Korean fermented cabbage. Microorganisms, 10(10), 1-15.

Srionnual, S. (2019). Screening of bacteriocin-producing lactic acid bacteria from southern Thailand traditional fermented seafood products for growth inhibition of foodborne pathogens. In Fiscal Year 2018 Grant Research Report. Rajamangala University of Technology Srivijaya, Nakhon Si Thammarat Campus.

Charteris, W. P., Kelly, P. M., Morelli, L., and Collins, J. K. (1998). Antibiotic susceptibility of potentially probiotic Lactobacillus species. Journal of Food Protection, 61(12), 1636-1643.

Woo, P. C. Y., Lau, S. K. P., Teng, J. L. L., Tse, H., and Yuen, K. –Y. (2008). Then and now: Use of 16S rDNA gene sequencing or bacterial identification and discovery of novel bacteria in clinical microbiology laboratories. Clinical Microbiology and Infection, 149(10), 908-934.

Sahadeva, R. P. K., Leong, S. F., Chua, K. H., Tan, C. H., Chan, H. Y., Tong, E. V., Wong, S. Y. W., and Chan, H. K. (2011). Survival of commercial probiotic strains to pH and bile. International Food Research Journal, 18(4), 1515-1522.

Khempaka, S. (2020). Evaluation of the efficacy of Lactobacillus and Bifidobacterium strains from Broiler gastrointestinal tract for use as probiotics in broiler diets. In Fiscal Year 2016 Grant Research Report. Academy of science Suranaree University of Technology, Nakhon Ratchasima.

Marutpong, P., Lulitanon, W., Rattanachaikunsopon, P., Sriwaramat, T., Chaiwong, T., and Tinbanmai, T. (2014). Probiotic characterization of lactic acid bacteria isolated from infant feces. Journal of Science and Technology Ubon Ratchathani University, 16(3), 38-45. (in Thai)

Begley, M., Hill, C., and Gahan, C. G. M. (2006). Bile salt hydrolase activity in probiotics. Applied and Environmental Microbiology, 72(3), 1729-1738.

Ruiz, L., Margolles, A., and Sanchez, B. (2013). Bile resistance mechanisms in Lactobacillus and Bifidobacterium. Frontiers in Microbiology, 396(4), 1-8. https://doi.org/10.3389/fmicb.2013.00396

Shehata, M. G., El Sohaimy, S. A., El-Sahn, M. A., and Youssef, M. M. (2016). Screening of isolated potential probiotic lactic acid bacteria for cholesterol lowering property and bile salt hydrolase activity. Annals of Agricultural Sciences, 61(1), 65-75.

Rastall, R. A. (2004). Bacteria in the gut: Friends and foes and how to alter the balance. The Journal of Nutrition, 134(8), 2022-2026.

Fijan, S. (2014). Microorganisms with claimed probiotic properties: An overview of recent literature. International Journal of Environmental Research and Public Health, 11(5), 4745-4767.

Campana, R., Hemert, S., and Baffone, W. (2017). Strain-specific probiotic properties of lactic acid bacteria and their interference with human intestinal pathogens invasion. Gut pathogens, 9, 1-12. https://doi.org/10.1186/s13099-017-0162-4

Bu, Y., Liu, Y., Liu, Y., Wang, S., Liu, Q., Hao, H., and Yi, H. (2022). Screening and probiotic potential evaluation of bacteriocin-producing Lactiplantibacillus plantarum in vitro. Foods, 11, 1-12.

Franz, C. M., Huch, M., Abriouel, H., Holzapfel, W., and Gálvez, A. (2011). Enterococci as probiotics and their implications in food safety. International Journal of Food Microbiology, 151(2), 125-140.

Bednorz, C., Guenther, S., Oelgeschläger, K., Kinnemann, B., Pieper, R., Hartmann, S., Tedin, K., Semmler, T., Neumann, K., Schierack P., Bethe, A., and Wieler L. H. (2013). Feeding the probiotic Enterococcus faecium strain NCIMB 10415 to piglets specifically reduces the number of Escherichia coli pathotypes that adhere to the gut mucosa. Applied and Environmental Microbiology, 79, 7896-7904.

Cao, G. T., Zeng, X. F., Chen, A. G., Zhou, L., Zhang L., Xiao, Y. P., and Yang C. M. (2013). Effects of a probiotic, Enterococcus faecium, on growth performance, intestinal morphology, immune response, and cecal microflora in broiler chickens challenged with Escherichia coli K88. Poultry Science, 92, 2949-2955.

Downloads

Published

2025-06-01

How to Cite

Srionnual, S., Kaewthai Andre, N., Chaipetch, S., & Torasin, T. . (2025). Evaluation of primary probiotic properties of bacteriocin-producing lactic acid bacteria isolated from Southern Thailand fermented foods. Srinakharinwirot University Journal of Sciences and Technology, 17(1, January-June), 1–13, Article 256217. retrieved from https://ph02.tci-thaijo.org/index.php/swujournal/article/view/256217