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This study conducted to develop a method for improving tenderness of beef meat using proteinases from tilapia (Oreochromis niloticus Linneaus) viscera at different concentrations (1, 2, 3, and 4 % (w/v)). Their tenderizing efficacy was compared with the most popular enzyme, papain. Electrophoretic pattern of muscle proteins revealed extensive proteolysis and reduction in number of small protein bands in all treated samples. Reduction (p < 0.05) in shear force values were observed in enzyme-treated samples compared to control. Viscera extract-treated meat samples (4 % w/v) received better scores for appearance, flavor, juiciness, tenderness and overall acceptability. These results indicated proteinases from viscera of tilapia can be used as an effective alternative to papain.
Shahidi, F. (1994). Proteins from Seafood Processing Discard. In E.Z. Sikorski, B.S. Pan & F. Shahidi, (Eds.). Seafood Proteans. 171-193. New York, USA : Chapman & Hall.
Shahidi, F., & Kamil, Y. Y. A. J. (2001). Enzymes from Fish and Aquatic Invertebrates and Their Application in Food Industry. Trends in Food Science and Technology, 12, 435-464.
Naveena, B. M., Kiran, M., Reddy, K. S., Ramakrishna, C., Vaithiyanathan, S., & Devatkal, S. K. (2011). Effect of Ammonium Hydroxide on Ultrastructure and Tenderness of Buffalo Meat. Meat Science, 88, 727–732.
Klomklao, S., Benjakul, S., & Visessanguan, W. (2004). Comparative Studies on Proteolytic Activity of Spleen Extracts from Three Tuna Species Commonly Used in Thailand. Journal of Food Biochemistry, 28, 355-372.
Klomklao, S., Kishimura, H., Benjakul, S., & Simpson, B.K. (2009). Autolysis and Biochemical Properties of Endogenous Proteinase in Japanese Sandfish (Arctoscopus japonicus). International Journal of Food Science and Technology, 44, 1344-1350.
Laemmli, U.K. (1970). Cleavage of Structure Proteins during the Assembly of the Head of Bacteriophage T4. Nature. 277, 680-685.
Zhao, G. Y., Zhou, M. Y., Zhao, H. L., Chen, X. L., Xie, B. B., Zhang, X. Y., He, H. L., Zhou, B.C., & Zhang, Y. Z.(2012). Tenderization Effect of Cold-adapted Collagenolytic Protease MCP-01 on Beef Meat at Low Temperature and Its Mechanism. Food Chemistry, 134, 1738-1744.