Isolation and Identification of Organic Acid-Producing Bacteria from Xylose

Abstract

The objective of present study is to isolate and identify the bacteria that capable of producing organic acid from xylose. A total of 401 samples were collected from Thailand and inoculated to basal medium containing 10% (v/v) xylose as the carbon source at 37^๐C. Microorganisms that grown and produced organic acid were purified by cross - streaking on an agar plate. The single colony was chosen for organic acid production by size of clear zone. The isolate LK34 produced malic acid as a major product with a high concentration at 35.381 g/L and productivity 0.491 g/l/h. Nucleotide sequences analysis of 16S rRNA showed that LK34 is more similar to Klebsiella sp. (sequence identity, 99%). This is the first report of genus Klebsiella that can produce malic acid from xylose.