Phytochemical Screening Test, Antioxidant Activity and Antibacterial Activity of Crude Extract of Vitex rotundifolia L.f. Leaves

Abstract

Phytochemical screening of the 95% ethanolic extracts from the dried leaves of Vitex rotundifolia L.f revealed the presence of alkaloids and tannin. The total phenolics content and antioxidant activity were evaluated according to Folin-Ciocalteu method and DPPH assay, respectively. The results showed that total phenolics content was 54.408 2.78 mg GAE/g extract. The IC₅₀ of ethanolic extracts was 133.97 μg/ml. The antibacterial activity were evaluated according to Disc Diffusion method. The results showed that extract not exhibited antibacterial activity against Escherichia coli and Staphylococus aureus at concentrations 20, 40 and 80 mg/ml