Influence of carbon and nitrogen sources on the performance of Polyhydoxyalkanoates (PHA) production using Novosphingobium sp. THA_AIK7 in batch operation

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Jantima Teeka, et al


The effect of various carbon and nitrogen sources on the growth and Polyhydoxyalkanoates (PHA) production of Novosphingobium sp. THA_AIK7 were investigated. Novosphingobium sp. THA_AIK7 was grown on Mineral salt medium (MSM) supplemented with four different carbon (molasses, starch hydrolysate, glucose and crude glycerol) and nitrogen sources (ammonium sulfate, ammonium chloride, urea and monosodium glutamate (MSG)). Crude glycerol with MSG was chosen as the best source for PHA production as it produced a flexible and white PHA casted film. This condition gave PHA concentration, PHA content and Yp/s of 0.407 g/L, 14.59% of dry cell weight and 0.298 g PHA/g crude glycerol after 96 h, respectively.

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