Biomass Productivity and Antioxidant Composition of Marine Microalga Tisochrysis sp. TISTR 11470 Under Outdoor Cultivation Condition
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This study aimed to evaluate the growth performance and biochemical composition of the marine microalga Tisochrysis sp. TISTR 11470 cultivated under outdoor cultivation using white rectangular plastic tanks with a volume of 600 L over a 9-day period. The results revealed a continuous increase in cell density, as indicated by the optical density at 750 nm (OD750), which rose from 0.10 on day 1 to 0.50 on day 9. The culture exhibited a highest specific growth rate of 0.58 day-1 at day 1, reflecting efficient photosynthetic adaptation under outdoor conditions. The pH increased from 8.54 to 9.33, while salinity rose from 15 to 19 ppt, which was associated with photosynthetic activity and water evaporation under outdoor conditions. The total biomass yield reached 1,275 g (fresh weight) or 24.84 g (dry weight; DW.), with an average productivity of 0.81 g DW m-2day-1. Biochemical analysis revealed that the microalgal biomass contained chlorophyll a at 2.47 ± 0.03 mg g-1 DW and total carotenoids at 1.90 ± 0.23 mg g-1 DW. The protein content was 11.48 ± 0.95 mg g-1 DW and carbohydrate content was 127.46±17.41 mg g-1 DW. Additionally, total phenolic content was 5.29±0.02 mg gallic acid equivalent (GAE) g-1DW, and total flavonoid content was 16.66±2.17 mg quercetin equivalent (QE) g-1DW, indicating a high level of antioxidant potential. The results of this study indicate the feasibility of cultivating Tisochrysis sp. TISTR 11470 in outdoor systems. However, further investigations are required to optimize key cultivation parameters, such as light intensity, temperature, and nutrient composition, in order to enhance growth performance, ensure production consistency, and improve its potential for industrial-scale applications.
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ลิขสิทธ์ ของมหาวิทยาลัยเทคโนโลยีราชมงคลพระนครReferences
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