In vitro Seed Propagation and Effect of BA and NAA on Plantlet Regeneration of Coelogyne viscosa Rchb.f.

Abstract

The objectives of this study were to optimize the culture medium and sucrose level for seeds germination and investigate the effect of BA and NAA on plantlet growth of (Coelogyne viscosa Rchb.f.). Orchid seeds were inoculated on Murashige and Skoog (MS, 1962) and 1/2MS media with three variable sucrose level (15, 20 and 30 g/L) for 8 and 12 weeks. After that, protocorm were cultured on 1/2MS medium with 20 g/L sucrose supplemented with different concentrations of BA (0, 1, 2 and 4 mg/L) and NAA (0, 0.5 and 1 mg/L) for 9 weeks. In order that, all experiments were cultured under standard conditions: 16-h photoperiod with cool white light 3,000 lx at 25±2˚C with completely randomized design (CRD). The results showed that after 12 weeks, orchid seeds successfully germinated on 1/2MS medium with 20 g/L sucrose with protocorm score was 5.00±0.00. Moreover, the results of plantlets were cultured on 1/2MS medium with 20 g/L sucrose supplemented with different concentrations of BA and NAA for 9 weeks showed the highest plantlet regeneration when cultured on the medium supplemented with BA 2 mg/mL and NAA 0.5 mg/mL. This study is orchid propagation by seed tissue culture which is an alternative methods that can further be used for Thailand orchids micropropagation and conservation.