Methylene blue decolorization using bead immobilized Thunbergia laurifolia Linn. leaf extract
Keywords:
Decolorization, Thunbergia laurifolia, BeadAbstract
Methylene blue is a dye using for textile cotton wool silk and leather industries which causes coloring mill effluent resulting in bad effects to organisms and aquatic ecosystem. The objective of this study examined ability of bead immobilized Thunbergia laurifolia Linn. leaf extract to methylene blue decolorize. The polyphenol oxidase, the decolorize enzyme, was also determined. The crude leaf extract of middle leaf stage showed highest polyphenol oxidase activity with 21,911.25±270.08 unit/ml %immobilization using 1.3% w/v calcium alginate was 18.03±1.10%. It could decolorize methylene blue within 60 min with % decolorization of 91.35±1.41. The bead also decolorized methylene blue after incubation in various pHs and temperatures for 30 min. The optimum pH and temperature was pH 10 and 70°C with %decolorization of 95.80 ±0.70 and 87.74±1.41, respectively. The bead immobilized Thunbergia laurifolia Linn. was reusable for 20 repeated cycles with 59.43±8.72% decolorization. These result suggested that bead immobilized Thunbergia laurifolia Linn leaf extract has a high potential to apply for color mill effluent treatment from many textile industries.
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