Quality control, cytotoxicity and inhibitory effect on nitric oxide production of Pathavi Apo Vayo formulary extract
คำสำคัญ:
Pathavi Apo Vayo formulary, Quality control, Medicinal plants, High glucose, Cytotoxicityบทคัดย่อ
Pathavi Apo Vayo formulary (PAV) is one of Thai traditional medicine that consisted of 21 herbal plant powders and has been used in the treatment of diabetic patients. An uncertainty on efficacy, safety and variation in quality of the products are important factors for herbal medicine usage. Therefore, the aim of this study was to establish quality control of PAV. The effects of PAV on cell viability and nitric oxide production in the different glucose containing media were also investigated. The 21 herbal plants and PAV were extracted using 50% ethanol. The possible chemical fingerprint and chemical markers of these plants were identified by using TLC and HPLC comparing with PAV. Normal glucose medium (NGM) and high glucose medium (HGM) were used to culture RAW264.7 cells in this study. The cytotoxicity and nitric oxide (NO) production were studied by using MTT assay and Griess reaction, respectively. The Rf values of 21 plant extracts on TLC fingerprint were similar with the Rf values of PAV extract. Gallic acid was the major content in PAV and plant extracts using qualitative HPLC. PAV extract showed low cytotoxicity (IC50 of 1,139.48 ± 36.22 and 1,134.69 ± 13.55 µg/mL, respectively) and could inhibit NO production (IC50 of 128.49 ± 4.68 and 127.57 ± 14.02 µg/mL, respectively) in NGM and HGM without a statistical difference. In conclusion, the chemical composition of plant extracts was remaining content in PAV extract and gallic acid can be used as a chemical marker. This PAV extract had low cytotoxicity and inhibited NO production.
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