Quality control, cytotoxicity and inhibitory effect on nitric oxide production of Pathavi Apo Vayo formulary extract

ผู้แต่ง

  • Bung-on Prajanban Faculty of Agricultural Technology, Burapha University Sakaeo Campus
  • Orapun Jaisamut Faculty of Science and Technology, Rajamangala University of Technology Tawa-Ok
  • Niramai Fangkrathok Center for Research and Development of Herbal Health Products, Faculty of Pharmaceutical Sciences

คำสำคัญ:

Pathavi Apo Vayo formulary, Quality control, Medicinal plants, High glucose, Cytotoxicity

บทคัดย่อ

Pathavi Apo Vayo formulary (PAV) is one of Thai traditional medicine that consisted of 21 herbal plant powders and has been used in the treatment of diabetic patients. An uncertainty on efficacy, safety and variation in quality of the products are important factors for herbal medicine usage. Therefore, the aim of this study was to establish quality control of PAV. The effects of PAV on cell viability and nitric oxide production in the different glucose containing media were also investigated.  The 21 herbal plants and PAV were extracted using 50% ethanol. The possible chemical fingerprint and chemical markers of these plants were identified by using TLC and HPLC comparing with PAV. Normal glucose medium (NGM) and high glucose medium (HGM) were used to culture RAW264.7 cells in this study. The cytotoxicity and nitric oxide (NO) production were studied by using MTT assay and Griess reaction, respectively. The Rf values of 21 plant extracts on TLC fingerprint were similar with the Rf values of PAV extract. Gallic acid was the major content in PAV and plant extracts using qualitative HPLC. PAV extract showed low cytotoxicity (IC50 of 1,139.48 ± 36.22 and 1,134.69 ± 13.55 µg/mL, respectively) and could inhibit NO production (IC50 of 128.49 ± 4.68 and 127.57 ± 14.02 µg/mL, respectively) in NGM and HGM without a statistical difference. In conclusion, the chemical composition of plant extracts was remaining content in PAV extract and gallic acid can be used as a chemical marker. This PAV extract had low cytotoxicity and inhibited NO production.

Downloads

Download data is not yet available.

References

Adela R, Nethi SK, Bagul PK, Barui AK, Mattapally S, Kuncha M, et al. Hyperglycaemia enhances nitric oxide production in diabetes: a study from South Indian patients. PloS one 2015;10(4):e0125270.

Torres SH, De Sanctis JB, de Briceno LM, Hernandez N, Finol HJ. Inflammation and nitric oxide production in skeletal muscle of type 2 diabetic patients. J Endocrinol 2004;181(3):419-27.

Guo H, Zhang Q, Yuan H, Zhou L, Li FF, Wang SM, et al. Nitric oxide mediates inflammation in type II diabetes mellitus through the PPARγ/eNOS signaling pathway. PPAR research 2020;2020:1-7.

Ozcelik O, Algul S. Nitric oxide levels in response to the patients with different stage of diabetes. Cell Mol Biol (Noisy-le-grand) 2017;63(1):49-52.

Chatterjee S, Riewpaiboon A, Piyauthakit P, Riewpaiboon W, Boupaijit K, Panpuwong N, et al. Cost of diabetes and its complications in Thailand: a complete picture of economic burden. Heal Soc Care Community 2011;19(3):289–98.

Harrigan RA, Nathan MS, Beattie P. Oral agents for the treatment of type 2 diabetes mellitus: pharmacology, toxicity, and treatment. Annu Emerg Med 2001;38(1):68–78.

Moolasarn S, Sripa S, Kuessirikiet V, Sutawee K, Huasary J, Chaisila C. Usage of and cost of complementary/alternative medicine in diabetic patients. J Med Assoc Thai 2005;88(11):1630–7.

Kumar S, Mittal A, Babu D, Mittal A. Herbal medicines for diabetes management and its secondary complications. Curr Diabetes Rev 2021;7(4):437–56.

Eloff JN, Ntloedibe DT, van Brummelen R. A simplified but effective method for the quality control of medicinal plants by planar chromatography. Afr J Tradit Complement Altern Med 2011;8(5 Suppl):1–12.

Singh S, Verma V, Yadav R, Singh B. Pharmacognostical study of Amalaki (Emblica officinalis Gaertn.). J Pharmacogn Phytochem 2018;7(3):3476–80.

Muthuraman A, Sood S, Singla SK. The antiinflammatory potential of phenolic compounds from Emblica officinalis L. in rat. Inflammopharmacology 2011;19(6):327–34.

Theepireddy SKR, Chinthala R, Rao LV, Kurisetty VV, Kura RR, Duvvada MR. The isolation, characterization and quantification of gallic acid from the fruit extract of Terminalia chebula. JMPR 2015;3(2):983–8.

Peungvicha P, Vallisuta O, Mangmool S, Sirithamwanich T, Sirithamwanich R. Anti-hyperglycemic effect and subchronic toxicity of the combined extract from Sattagavata-Mathurameha-Tubpikarn anti-diabetic herbal formulae. Thai J Pharm Sci 2018;42(1):6-13.

Torres- Castro I, Arroyo- Camarena UD, Martínez-Reyes CP, Gómez- Arauz AY, Due˜nas- Andrade T, Hernández-Ruiz J, et al. Human monocytes and macrophages undergo M1-type inflammatory polarization in response to high levels of glucose. Immunol Lett 2016;176:81–9.

Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65(1-2):55–63.

Ardalani H, Hejazi Amiri F, Hadipanah A, Kongstad, KT. Potential antidiabetic phytochemicals in plant roots: A review of in vivo studies. J Diabetes Metab Disord 2021;20(2):1837–54.

Balekundri A, Mannur V. Quality control of the traditional herbs and herbal products: a review. Futur J Pharm Sci 2020;6(1):1-9.

Amakura Y, Yoshimura M, Mouri C, Mikage M, Kawahara N, Goda Y, et al. Convenient TLC-based identification test for the crude drug “Pogostemoni Herba”. Yakugaku Zasshi 2008;128(12):1833-7.

Lin D, Xiao M, Zhao J, Li Z, Xing B, Li X, et al. An Overview of Plant Phenolic Compounds and Their Importance in Human Nutrition and Management of Type 2 Diabetes. Molecules 2016;21(10):1374.

Singh B, Kumar A, Singh H, Kaur S, Arora S, Singh B. Protective effect of vanillic acid against diabetes and diabetic nephropathy by attenuating oxidative stress and upregulation of NF-κB, TNF-α and COX-2 proteins in rats. Phytother Res 2022;36(3):1338–52.

Xu Y, Tang G, Zhang C, Wang N, Feng Y. Gallic acid and diabetes mellitus: its association with oxidative stress. Molecules 2021;26(23):7115.

Salau VF, Erukainure OL, Ijomone OM, Islam MS. Caffeic acid regulates glucose homeostasis and inhibits purinergic and cholinergic activities while abating oxidative stress and dyslipidaemia in fructose-streptozotocin-induced diabetic rats. J Pharm Pharmacol 2022;74(7):973–84.

Chao CY, Mong MC, Chan KC, Yin MC. Anti-glycative and anti-inflammatory effects of caffeic acid and ellagic acid in kidney of diabetic mice. Mol Nutr Food Res 2010;54(3):388–95.

Chawananorasest K, Pikulthong A, Meeploy M, Munyanont M, Jitprom N, Banjongsinsiri P, et al. Total phenolic and gallic acid contents in fresh and preserved Emblica officinalis Gaertn fruits from five different varieties. Srinakharinwirot University (Journal of Science and Technology) 2019;11(22):13–22.

Saha S, Verma RJ. Antioxidant activity of polyphenolic extract of Terminalia chebula Retzius fruits. J Taibah University for Science 2016;10(6):805–12.

Makihara H, Koike Y, Ohta M, Horiguchi-Babamoto E, Tsubata M, Kinoshita K, et al. Gallic acid, the active ingredient of Terminalia bellirica, enhances adipocyte differentiation and adiponectin secretion. Biol Pharm Bull 2016;39(7):1137–43.

Dhanasehian A, Srivani S, Rameshkumar MR. Nitric oxide production and antioxidant activity of dried fruit extracts of Terminalia chebula. Asian J Pharm Clin Res 2018;11(5):370–6.

Hazra B, Sarkar R, Biswas S, Mandal N. Comparative study of the antioxidant and reactive oxygen species scavenging properties in the extracts of the fruits of Terminalia chebula, Terminalia belerica and Emblica officinalis. BMC Complement Altern Med 2010;10(1):1–15.

Kumar GPS, Arulselvan P, Kumar DS, Subramanian SP. Anti-diabetic activity of fruits of Terminalia chebula on streptozotocin induced diabetic rats. J Health Sci 2006;52(3):283-91.

Aung EPP, Lwin SH, Aye NN, Phyu KP. Hypoglycemic Effect of Terminalia chebula retz. Fruit on alloxan-induced diabetic rats. Siriraj Medical Journal 2017;69(2):80–4.

Variya BC, Bakrania AK, Patel SS. Antidiabetic potential of gallic acid from Emblica officinalis: Improved glucose transporters and insulin sensitivity through PPAR-γ and Akt signaling. Phytomedicine 2020;73:152906.

Abdulrazaq NB, Cho MM, Win NN, Zaman R, Rahman MT. Beneficial effects of ginger (Zingiber officinale) on carbohydrate metabolism in streptozotocin-induced diabetic rats. Br J Nutr 2012;108(7):1194–201.

Singh P, Khosa RL, Mishra G, Jha KK. Antidiabetic activity of ethanolic extract of Cyperus rotundus rhizomes in streptozotocin-induced diabetic mice. J Pharm Bioallied Sci 2015;7(4):289–92.

Sudatri NW, Warasiti N, Suartini NM, Bidura NI. Anti- diabetic and anti-cholesterol activity of Kaempferia galangal L. herbal medicine rhizome in albino rats. Int J Fauna Biol Stud 2019;6(5):13–7.

Ashraf R, Khan RA, Ashraf I. Garlic (Allium sativum) supplementation with standard antidiabetic agent provides better diabetic control in type 2 diabetes patients. Pak J Pharm Sci 2011;24(4):565–70.

Cahyaningrum PL, Yuliari SAM, Suta IBP. Antidiabetic Activity Test Using Amla Fruit (Phyllanthus Emblica L) Extract in Alloxan-Induced Balb/C Mice. J Vocational Health Stud 2019;3(2):53–8.

Saha P, Mazumder UK, Haldar PK, Sen SK, Naskar S. Antihyperglycemic activity of Lagenaria siceraria aerial parts on streptozotocin induced diabetes in rats. Diabetol Croat 2011;40(2):49–60.

Isdadiyanto S, Mardiati SM, Sitasiwi AJ. Blood-glucose levels of rats given high-fat diets after administration of neem leaf ethanolic extract. Biosaintifika 2021;13(2):142–8.

Latha RC, Daisy P. Insulin-secretagogue, antihyperlipidemic and other protective effects of gallic acid isolated from Terminalia bellerica Roxb. in streptozotocin-induced diabetic rats. Chem Biol Interact 2011;189(1-2):112–8.

Variya BC, Bakrania AK, Patel SS. Antidiabetic potential of gallic acid from Emblica officinalis: improved glucose transporters and insulin sensitivity through PPAR-γ and Akt signaling. Phytomedicine 2020;73: 152906.

Downloads

เผยแพร่แล้ว

2022-12-07