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The standardized karyotype and idiogram of the glass goby fish, Gobiopterus chuno (Hamilton, 1822) from Singburi Province, Thailand, were studied. Kidney (and testis in males) tissues were collected from ten female and ten male samples after soaking in the colchicine solution. Conventional staining (20% Giemsa solution) and Ag-NOR banding techniques (50% silver nitrate and 2% gelatin solution) were applied to stain the chromosomes. The results showed that diploid chromosome number of G. chuno was 2n= 48, and the fundamental number (NF) was 50 in both males and females. No strange sized chromosomes associated with sex were identified. Karyotype consisted of two large acrocentric, 24 large telocentric, 20 medium telocentric, and two small telocentric chromosomes. Positions of Nucleolar Organizer regions (NORs) were observed at the region adjacent to the telomeres of short arm of the chromosome pair 2 (acrocentric). The karyotype formula for G. chuno is as follows: 2n (diploid) 48=La2+Lt24+Mt20+St2
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