Simultaneous alcoholic and acetic acid fermentation in pineapple juice with co-culture of yeast and acetic acid bacteria

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On-ong Chanprasartsuk
Kittaphat Jaroenmaneesap
Krittapoj Wongjinda


Co-culture of yeast and acetic acid bacteria (AAB) is consortium which be useful on food and beverage fermentation. The various fermented beverages; alcoholic and non-alcoholic drinks, are obtained by these microbial communities and their interactions. The activities of co-culture of yeasts and acetic acid bacteria during pineapple juice fermentation, including the growth of starters, physicochemical and key compounds changes during pineapple juice fermentation were investigated. The specific characteristics of initial pineapple juice was appropriate for using as a raw material for single and dual yeast and AAB fermentation with no chaptalization. The adding of AAB could encourage the growth and ethanol production of allochthonous and autochthonous yeasts during pineapple juice fermentation, concurrently, the growth of AAB slowly decreased throughout the fermentation. In order to better understand the relationship between yeast and AAB during pineapple juice fermentation. The influence of pineapple juice properties on the growth of AAB during pineapple fermentation was further investigated.

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How to Cite
Chanprasartsuk, O.- ong, Jaroenmaneesap, K. ., & Wongjinda, K. (2023). Simultaneous alcoholic and acetic acid fermentation in pineapple juice with co-culture of yeast and acetic acid bacteria. Food Agricultural Sciences and Technology, 9(3), 1–12. Retrieved from


Ajibade, V.A., Akinruli, F.T., & Ilesanmi, T.M. (2015). Antibacterial screening of crude extract of oven-dried pawpaw and pineapple. International Journal of Scientific and Research Publications, 5, 408-411.

Chanprasartsuk, O., Chamnoiprom, C., Charoenchai, K., Phunpheng, S., Khamee, S., & Panupintu, S. (2020). Dynamic changes of physicochemical properties of pineapple juice during fermentation with allochthonous and autochthonous yeasts under different conditions. Science Technology and Engineering Journal, 6(1), 67-77.

Chanprasartsuk, O., Pheanudomkitlert, K., & Toonwai, D. (2012). Pineapple wine fermentation with yeasts isolated from fruit as single and mixed starter cultures. Asian Journal of Food and Agro-Industry, 5(2), 104-111.

Chanprasartsuk, O., & Prakitchaiwattana, C. (2022). Growth kinetics and fermentation properties of autochthonous yeasts in pineapple juice fermentation for starter culture development. International Journal of Food Microbiology, 371, 109636.

Ciani, M., Comitini,F., Mannazzu, I., & Domizio, P. (2010). Controlled mixed culture fermentation: a new perspective on the use of non-Saccharomyces yeasts in winemaking. FEMS Yeast Research, 10(2), 123-133.

Colletti, A., Li, S., Marengo, M., Adinolfi, S., & Cravotto, G. (2021). Recent advances and insights into bromelain processing, pharmacokinetics and therapeutic uses. Applied Sciences, 11, 8428.

de Ancos, B., Sánchez-Moreno, C., & González-Aguilar, G.A. (2017). Pineapple composition and nutrition. In M.G., Lobo & R.E. Paull (Eds.), Handbook of Pineapple Technology: Production, Postharvest Science, Processing and Nutrition. https:// ch12

Desiderio, W., & Escalante, E. (2018). Perspectives and uses of non-saccharomyces yeasts in fermented beverages. In R., Lidia Solís-Oviedo, & Á., de la Cruz Pech-Canul (Eds.), Frontiers and new trends in the science of fermented food and beverages. https://doi:10.5772/intechopen. 73404.

Ferreira, A.M. & Mendes-Faia, A. (2020). The role of yeasts and lactic acid bacteria on the metabolism of organic acids during winemaking. Foods, 9, 1231, https://doi:10.3390/ foods9091231

George, S., Bhasker, S., Madhav, H., Nair, A., & Chinnamma, M. (2013). Functional characterization of recombinant bromelain of Ananas comosus expressed in a prokaryotic system. Molecular Biotechnology, 56, 166-174.

Grassi, A., Cristani C., Palla, M., Di Giorgi, R., Giovannetti, M., & Agnolucci, M. (2022). Storage time and temperature affect microbial dynamics of yeasts and acetic acid bacteria in a kombucha beverage. International Journal of Food Microbiology, 382, 109934.

Gullo, M., Caggia, C., De Vero, L., & Giudici, P. (2006). Characterization of acetic acid bacteria in “traditional balsamic vinegar”. International Journal of Food Microbiology, 106(2), 209-12.

He, Y., Xie, Z., Zhang, H., Liebl, W., Toyama, H., & Chen, F. (2022). Oxidative fermentation of acetic acid bacteria and its products. Frontiers in Microbiology, 13, https://doi. org/10.3389/fmicb.2022.879246

Jayabalan, R., Marimuthu, S., & Swaminathan, K. (2007). Changes in content of organic acids and tea polyphenols during kombucha tea fermentation. Food Chemistry, 102, 392-398.

Jost, P., & Piendl, A. (1975). Technological influences on the formation of acetate during fermentation. Journal of the American Society of Brewing Chemists, 34, 31–37.

Khattab, S.M.R., & Kodaki, T. (2016). A novel production method for high-fructose glucose syrup from sucrose-containing biomass by a newly isolated strain of osmotolerant Meyerozyma guilliermondii. Journal of Microbiology and Biotechnology, 26(4), 675-683.

Kumar, V., & Joshi V. (2016). Kombucha: technology, microbiology, production, composition and therapeutic value. International Journal of Food and Fermentation Technology, 6, 13-24.

Kurtzman, C.P., & Robnett, C.J. (1998). Identification and phylogeny of ascomycetous yeasts from analysis of nuclear large subunit (26S) ribosomal DNA partial sequences. Antonie Van Leeuwenhoek, 73, 331-371.

Liu, C.-H., Hsu, W.-H., Lee, F.-L., & Liao, C.-C. (1996). The isolation and identification of microbes from a fermented tea beverage, Haipao, and their interactions during Haipao fermentation. Food Microbiology, 13, 407-415.

Mameli, A., Natoli, V., & Casu, C. (2020). Bromelain: an overview of applications in medicine and dentistry. Biointerface Research in Applied Chemistry, 11, 8165-8170.

Mamo, J., & Assefa, F. (2019). Antibacterial and anticancer property of bromelain: a plant protease enzyme from pineapples (Ananas comosus). Current Trends in Biomedical Engineering & Biosciences, 19, 60-68.

Mathew, B., Agrawal, S., Nashikkar, N., Sunita, B., & Upadhyay, A. (2019). Isolation of acetic acid bacteria and preparation of starter culture for apple cider vinegar fermentation. Advances in Microbiology, 9(6). https://doi:10.4236/aim.2019.96034.

Mullins, E.A., Francois, J.A., & Kappock, T.J. (2008). A specialized citric acid cycle requiring succinyl-Coenzyme A (CoA): acetate CoA-transferase (AarC) confers acetic acid resistance on the acidophile Acetobacter aceti. Journal of Bacteriology, 190(14), 4933–4940.

Pavan, R., Jain, S., Shraddha, & Kumar, A. (2012). Properties and therapeutic application of bromelain: a review. Biotechnology Research International, 976203.

Ribéreau-Gayon, P., Dubourdieu, D., Donéche, B., & Lonvaud, A. (2006). Handbook of enology, volume 1: The microbiology of wine and vinifications (2nd ed.). John Wiley & Sons Ltd.

Shang, Y.-H., Zeng, Y.-J., Zhu, P. & Zhong, Q.-P. (2016). Acetate metabolism of Saccharomyces cerevisiae at different temperatures during lychee wine fermentation. Biotechnology & Biotechnological Equipment, 30(3), 512-520.

Tallei, T.E., Fatimawali, A.Y., Idroes, R., Kusumawaty, D., Bin Emran, T., Yesiloglu, T.Z., Sippl, W., Mahmud, S., Alqahtani, T., & Alqahtani, A.M. (2021). An analysis based on molecular docking and molecular dynamics simulation study of bromelain as anti-sars-cov-2 variants. Frontiers in Pharmacology, 12, 717757.

The Government Public Relations Department. (2023). Thailand drives to retain its status as the world’s largest canned pineapple exporter 2023. https://thailand.prd. id/48/iid/174194

Tran, T., Grandvalet, C., Verdier, F., Martin, A., Alexandre, H. & Tourdot-Maréchal, R. (2020). Microbial dynamics between yeasts and acetic acid bacteria in kombucha: impacts on the chemical composition of the beverage. Foods, 9, 963. https://doi:10.3390/ foods9070963

Villarreal-Soto, S.A., Beaufort, S., Bouajila, J., Souchard, J.P., & Taillandier, P. (2018). Understanding kombucha tea fermentation: a review. Journal of Food Science, 83, 580-588. https:// doi: 10.1111/1750-3841.14068.

Vollmer, W., Blanot, D., & de Pedro, M.A. (2008). Peptidoglycan structure and architecture. FEMS Microbiology Reviews, 32, 149-167.