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This research aims to study karyotype of Pisang Awak (Kluai Namwa Tanao Sri, Musa × paradisiaca L.) from Suan Phueng district, Ratchaburi province. Chromosomes were obtained directly from the root tip and fixed roots; meristem cells were stained with acetocarmine dye which were then photographed under a light microscope for cells that were in the metaphase stage. Excel and the Adobe Photoshop CS5 application were used to analyze the data and display ideograms. The chromosome numbers of Musa × paradisiaca L. is 3n = 33 (x = 11), metacentric, submetacentric and acrocentric chromosome are commonly observed. The chromosome range length was reported between 0.742- 1.697 μm, karyotype comprising three large submetacentric, six large acrocentric, six medium metacentric, nine medium submetacentric, three medium acrocentric, three small metacentric and three small submetacentric chromosome, respectively. The karyotype formula of Kluai Namwa Tanao Sri (Musa × paradisiaca L.) as follows: 3n (33) = Lsm 3 + La 6 + Mm 6 + Msm 9 + Ma 3 + Sm 3 + Ssm 3
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