Prevalence and intensity of metacercariae in cyprinoid fish from Lam Pao Dam reservoir, Northeastern Thailand

Authors

  • Nipawan Du Souich Faculty of Sciences, Udon Thani Rajabhat University, Thailand
  • Chanaporn Rattanamalee Faculty of Sciences, Nakhon Panom University, Thailand
  • Wiwat Kaensa Faculty of Sciences, Udon Thani Rajabhat University, Thailand
  • Sakulak Siriso Faculty of Sciences, Udon Thani Rajabhat University, Thailand
  • Kultida Pausri Faculty of Sciences, Udon Thani Rajabhat University, Thailand

Keywords:

Prevalence, metacercariae, trematode, cyprinoid fish

Abstract

The northeast of Thailand is considered as an endemic area of trematode infection. The infective stage of trematode (metacercariae) is encysted in the cyprinoid fish which transmitted to human by raw fish ingestion. The aims of our study were to report the prevalence of the infected cyprinoid fish from Lam Pao Dam Reservoir and identify the metacercariae species. This research was conducted during May to August 2019. Fish species were identifed by morphological method then an individual samples were minced and mixed in 0.25% pepsin solution in 1% HCl. The suspension was fltered with a series of sieves (850, 300 mm) and washed with 0.85% NaCl. All metacercariae were identifed under a stereomicroscope and a compound light microscope. The total of 482 cyprinoid fish individuals were identifed as seven fish species namely Henicorhynchus siamensis, Puntioplites proctozystron, Barbonymus altus, Osteochilus vittatus, Osteochilus lini, Puntius brevis and Thynnichthys thynnoides. Metacercariae were found in fve of seven species which H. siamensis showed the highest percentage (8.18% of prevalence) with intensity was 13.61 metacercariae per fish. A total of 278 metacercariae were found and Haplorchis pumilio (88.13%) was the most abundance metacercariae. The infection of Opisthorchis viverrini and heterophyid metacercariae was also reported in this study. This research indicates the risk of human parasite infection when consuming raw fish.

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Published

15-10-2024

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Vol. 10 No. 3 (2024): SEPTEMBER - DECEMBER

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