Effect of water quality, sediment quality and microbial community on the growth of green mud crab (Scylla paramamosian) at different stocking densities
Keywords:
Mud crab, water quality, sediment quality, microbial community, stocking densityAbstract
The influence of water quality, sediment quality, and microbial community factors on the growth of mud crabs (Scylla paramamosian) at different stocking densities was examined. The experiment involved stocking mud crab culture at four densities: (T-1) 1 crab/m2, (T-2) 1.5 crab/m2, (T-3) 2 crab/m2, and (T-4) 3 crab/ m2, with triplicates for each density. The growth performance of the mud crab over a 101-day culture period was monitored. The survival rate at 1 crab/ m2 was significantly higher than at 2 and 3 crabs/m2 (P < 0.05). Additionally, the average production of 1 and 1.5 crabs/m2 was significantly higher than at 3 crabs/m2 (P < 0.05). Nitrite levels in sediment at the 3 crabs/m2 stocking density were significantly higher than at the 1 crab/m2 stocking density during the culture period. The dominant phyla found in the ponds were Phylum Cyanobacteria, Actinobacteria, and Bacteroidota, with no microbial pathogens detected. The recommended stocking densities for mud crab culture range between 1 crab/m2 and 1.5 crab/m2, as these densities are deemed suitable for maximizing productivity in mud crab farming
References
ACFS. (2012). Good aquaculture practices for blue swimming crab farm and mud crab farm. Ministry of Agriculture and Cooperatives, Bangkok.
Aji, M. B., Supriyono, E., & Soelistyowati, D. T. (2019). A preliminary study of the effect of alkalinity level on the survival rate and growth of the Panulirus homarus lobster. International Journal of Fisheries and Aquatic Studies, 7(4), 339-342. https://api.semanticscholar.org/CorpusID:202020760
Alfansah, Y. R., Hassenruck, C., Kunzmann, A., Taslihan, A., Harder, J., & Gardes, A. (2018). Bacterial abundance and community composition in pond water from shrimp aquaculture systems with different stocking densities. Frontiers in Microbiology, 9, 2457. https://doi.org/10.3389/fmicb.2018.02457
APHA, AWWA, & WEF. (1998). Standard methods for the examination of water and wastewater (20th ed.). United Book Press, Maryland.
Antony, J., Hussain, T., Patil, P. A., Biu, I. F., Mahalakshmi, P., Balasubramanian, C. P., Kailasam, M., & Ambasankar, K. (2019). Mud Crab Culture: A Gujarat Perspective. CIBA Extension Series No. 67. ICAR-CIBA, Chennai. (pp. 9).
Avnimelech, Y., & Ritvo, G. (2003). Shrimp and fish pond soils: Processes and management. Aquaculture, 220(1-4), 549-567. https://doi.org/10.1016/S0044-8486(02)00641-5
Bai, D., Li, X., Liu, Z., Wan, L., Song, C., Zhou, & Cao, X. (2023). Nitrogen and phosphorus turnover and coupling in ponds with different aquaculture species. Aquaculture, 563(2), 738997. https://doi.org/10.1016/j.aquaculture.2022.738997
Baliao, D. D., Rodriguez, E. M., & Gerochi, D. D. (1981). Culture of the mud crab, Scylla serrata (Forskal) at different stocking densities in brackishwater ponds. SEAFDEC Aquaculture Department Quarterly Research Report, 5(1), 10–14.
Bentzon-Tilia, M., Sonnenschein, E. C., & Gram, L. (2016). Monitoring and managing microbes in aquaculture – Towards a sustainable industry. Microbial Biotechnology, 9(5), 576-584.
Blake, G. R., & Hartge, K. H. (1986). Bulk density. In A. Klute (Ed.), Methods of Soil Analysis, Part 1—Physical and Mineralogical Methods (2nd ed., pp. 363-382). Agronomy Monograph 9. American Society of Agronomy—Soil Science Society of America. https://doi.org/10.2136/sssabookser5.1.2ed.c13
Boyd, C. E. (1995). Bottom Soils, Sediment, and Pond Aquaculture. Chapman & Hall Book.
Boyd, C. E. (1998). Water Quality in Ponds for Aquaculture. Auburn University Press.
Boyd, C. E., Wood, C. W., & Thunjai, T. (2002). Aquaculture Pond Bottom Soil Quality Management. Pond Dynamics/Aquaculture Collaborative Research Support Program, Oregon State University.
Boyd, C. E., & Tucker, C. S. (2014). Handbook for Aquaculture Water Quality. Craftmaster Printers, Inc.
Chuan, L. L., & Sugahara, I. (1984). A Manual on Chemical Analysis of Coastal Water and Bottom Sediment. Primary Production Department/Marine Fisheries Research Department.
Chen, F., & Wang, K. (2019). Characterization of the innate immunity in the mud crab Scylla paramamosain. Fish & Shellfish Immunology, 93, 436-448. https://doi.org/10.1016/j.fsi.2019.07.076
Chen, C., Xu, C., Qian, D., Yu, Q., Huang, M., Zhou, L., Qin, J. G., Chen, L., & Li, E. (2020). Growth and health status of Pacific white shrimp, Litopenaeus vannamei, exposed to chronic waterborne cobalt. Fish and Shellfish Immunology, 100, 137-145. https://doi.org/10.1016/j.fsi.2020.03.011
Chowdhury, R. M., Muntasir, S. Y., Naser, N., & Rafee, S. M. (2012). Water quality analysis of surface water bodies along the Dhaka-Maya-Bhanga road based on pre-monsoon water quality parameters for aquaculture. International Journal of Civil Engineering and Technology, 3(2), 154-168.
Deng, Y., Cheng, C., Feng, J., Liu, S., Ma, H., Chen, X., Chen, H., & Guo, Z. (2020). Rapid environmental change shapes pond water microbial community structure and function, affecting mud crab (Scylla paramamosain) survivability. Applied Microbiology and Biotechnology, 104(5), 2229-2241. https://doi.org/10.1007/s00253-019-10328-w
Department of Fisheries. (2018). Fisheries Statistics of Thailand. Fisheries Development Policy and Planning Division, Ministry of Agriculture and Cooperatives.
Fan, L., & Li, Q. X. (2019). Characteristics of intestinal microbiota in the Pacific white shrimp Litopenaeus vannamei differing growth performances in the marine cultured environment. Aquaculture, 505, 450-461. https://doi.org/10.1016/j.aquaculture.2019.02.075
Fazhan, H., Waiho, K., Wan Norfaizza, W. I., Megat, F. H., & Ikhwabuddin, M. (2017). Assortative mating by size in three species of mud crabs, genus Scylla De Haan, 1833 (Brachyura: Portunidae). Journal of Crustacean Biology, 3(5), 654-660. https://doi.org/10.1093/jcbiol/rux063
Funge-Smith, S. J., & Briggs, M. R. P. (1998). Nutrient budgets in intensive shrimp ponds: Implications for sustainability. Aquaculture, 164(1-4), 117-133. https://doi.org/10.1016/S0044-8486(98)00181-1
Hargreaves, J. A. (1998). Nitrogen biogeochemistry of aquaculture ponds. Aquaculture, 166(3-4), 181-212. https://doi.org/10.1016/S0044-8486(98)00298-1
Hong, M., Chen, L., Qin, J. G., Sun, X., Li, E., Gu, S., & Yu, N. (2009). Acute tolerance and metabolic responses of Chinese mitten crab (Eriocheir sinensis) juveniles to ambient nitrite. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 149(3), 419-426. https://doi.org/10.1016/j.cbpc.2008.10.001
Hossain, Md. B., Sultana, N., Noor, P., Khan, S., Lisa, A., Begum, M., Punom, N. J., Begum, Mst. K., Hasan, Md. R., & Rahman, M. S. (2017). Growth performance and fatty acid profile of Nile tilapia Oreochromis niloticus (Linnaeus, 1758) fed with different phytoplankton. Dhaka University Journal of Biological Sciences, 26(1), 13-27.
Huang, F., Pan, L., Song, M., Tian, C., & Gao, S. (2018). Microbiota assemblages of water, sediment, and intestine and their associations with environmental factors and shrimp physiological health. Applied Microbiology and Biotechnology, 102(19), 8585-8598. https://doi.org/10.1007/s00253-018-9229-5
Hubatsch, H. A., Lee, S. Y., Meynecke, J. O., Diele, K., Nordhaus, I., & Wolff, M. (2016). Life-history, movement, and habitat use of Scylla serrata (Decapoda, Portunidae): Current knowledge and future challenges. Hydrobiologia, 763, 5-21. https://doi.org/10.1007/s10750-015-2393-z
Hussenot, J., & Martin, L. M. (1995). Assessment of the quality of pond sediment in aquaculture using simple, rapid techniques. Aquaculture International, 3, 123-133. https://doi.org/10.1007/BF00117879
IBM Corp. (2020). IBM SPSS Statistics for Windows (Version 27.0) [Computer software].
Islam, S. T., Hossain, Md. Z., & Sultana, I. (2017). Challenges in mud crab production and marketing: A study on Khulna and Satkhira District. International Journal of Multidisciplinary Research and Development, 4(9), 206-212.
Keenan, C. P. (1999). Aquaculture of the Mud Crab, Genus Scylla—Past, Present and Future. Proceedings of an international scientific forum held in Darwin, Australia, 21–24 April 1997. ACIAR Proceedings No. 78, (pp. 9-13).
Mia, Md., Rahman Shah, M. M., Alam, J. M., & Begum, M. (2007). Optimization of the stocking density of mud crab, Scylla serrata culture in brackish water earthen ponds in Bangladesh. Sindh University Research Journal, 39(1), 25-32.
Mondal, A., Bhatacharya, S., Mitra, A., Sundaray, J. K., & Mohanty, R. K. (2020). Performance evaluation of mud crab Scylla olivacea (Herbst, 1896) co-culture with different fish species in confined brackish water ponds. Aquaculture, 552, 735125. https://doi.org/10.1016/j.aquaculture.2020.735125
Munni, M. A., Fardus, Z., Mia, M. Y., & Afrin, R. (2015). Assessment of pond water quality for fish culture: A case study of Santosh region in Tangail, Bangladesh. Journal of Environmental Science and Natural Resources, 6(2), 157-162. https://doi.org/10.3329/jesnr.v6i2.22112
Mutoti, M., Gumbo, J., & Jideani, A. I. O. (2022). Occurrence of cyanobacteria in water used for food production: A review. Physics and Chemistry of the Earth, Parts A/B/C, 125, 103101. https://doi.org/10.1016/j.pce.2021.103101
Nelson, D. W., & Sommer, L. E. (1982). Total carbon, organic carbon and organic matter. In A. L. Page, R. H. Miller, & D. R. Keeney (Eds.), Methods of Soil Analysis, Part 2: Chemical and Microbiological Properties (2nd ed., pp. 579-595). ASA-SSSA.
Nikhom, L. (2019). Water analysis manual for aquaculture and farm standard certification inspection. Narathiwat Marine Fisheries Research and Development, Fisheries Department.
Nooseng, S. (2015). Status of mud crab industry in Thailand. In F. D. Parado-Estepa, E. M. Avila, & J. C. Ladja (Eds.), Proceedings of the International Seminar-Workshop on Mud Crab Aquaculture and Fisheries Management (pp. 37-43). SEAFDEC Aquaculture Department. http://hdl.handle.net/10862/3208
Páez-Osuna, F., Guerrero-Galván, S. R., Ruiz-Fernández, A. C., & Espinoza-Angulo, R. (1997). Fluxes and mass balances of nutrients in a semi-intensive shrimp farm in north-western Mexico. Marine Pollution Bulletin, 34(5), 290-297. https://doi.org/10.1016/S0025-326X(96)00133-6
Rao, S. P. S., Karunasagar, I., & Ota, S. (2000). Incidence of bacteria involved in nitrogen and sulphur cycles in tropical shrimp culture ponds. Aquaculture International, 8, 463–472. https://doi.org/10.1023/A:1009250004999
Romano, N., & Zeng, C. (2016). Cannibalism of decapod crustaceans and implications for their aquaculture: A review of its prevalence, influencing factors, and mitigating methods. Reviews in Fisheries Science & Aquaculture, 25(1), 42–69. https://doi.org/10.1080/23308249.2016.1221379
Hedayati, S. A., Bagheri, T., Hoseinifar, S. H., & Van Doan, H. (2020). Growth performances and hemato-immunological responses of common carp (Cyprinus carpio Linnaeus, 1758) to fermented Aspergillus oryzae. Iranian Journal of Fisheries Sciences, 19(4), 749–756. https://doi.org/10.22092/ifs.2018.117403
Shelley, C., & Lovatelli, A. (2011). Mud crab aquaculture – A practical manual (FAO Fisheries and Aquaculture Technical Paper No. 567). Food and Agriculture Organization of the United Nations. https://www.fao.org/4/ba0110e/ba0110e.pdf
Steeby, J. A., Hargreaves, J. A., Tucker, C. S., & Kingsbury, S. (2004). Accumulation, organic carbon, and dry matter concentration of sediment in commercial channel catfish ponds. Aquacultural Engineering, 30(3–4), 115–126. https://doi.org/10.1016/j.aquaeng.2003.10.001
Thunjai, T., Boyd, C. E., & Boonyaratpalin, M. (2004). Bottom soil quality in tilapia ponds of different ages in Thailand. Aquaculture Research, 35(7), 698–705. https://doi.org/10.1111/j.1365-2109.2004.01072.x
Triño, A. T., Millamena, O. M., & Keenan, C. P. (1999). Monosex culture of the mud crab Scylla serrata at three stocking densities with Gracilaria as crab shelter. In C. P. Keenan & A. Blackshaw (Eds.), Mud crab aquaculture and biology: Proceedings of an international scientific forum held in Darwin, Australia, 21–24 April 1997 (pp. 61–66). Australian Centre for International Agricultural Research.
Wang, X., Huang, Z., Wang, C., Qi, C., Gu, Z., Li, E., G., J., & Chen, L. (2020). A comparative study on growth and metabolism of Eriocheir sinensis juveniles under chronically low and high pH stress. Frontiers in Physiology, 11, 885. https://doi.org/10.3389/fphys.2020.00885
Washim, M. M. R., Rubel, A. K. M. S., Ahmmed, S., Rahman, S. L., & Islam, M. L. (2022). Stocking density optimization of juvenile mud crab (Scylla olivacea) cultivation in bamboo-fetched earthen ponds. AACL Bioflux, 15(1), 384–394.
Weber, J. B. (1977). Soil properties, herbicide sorption, and model soil systems. In B. Truelove (Ed.), Research methods in weed science (2nd ed., pp. 59–62). Southern Weed Science Society.
Youssef, N., Sheik, C. S., Krumholz, L. R., Najar, F. Z., Roe, B. A., & Elshahed, M. S. (2009). Comparison of species richness estimates obtained using nearly complete fragments and simulated pyrosequencing-generated fragments in 16S rRNA gene-based environmental surveys. Applied and Environmental Microbiology, 75(16), 5227–5236. https://doi.org/10.1128/AEM.00592-09
Zhang, X., Tang, X., Tran, N. T., Huang, Y., Gong, Y., Zhang, Y., Zheng, H., Ma, H., & Li, S. (2019). Innate immune responses and metabolic alterations of mud crab (Scylla paramamosain) in response to Vibrio parahaemolyticus infection. Fish and Shellfish Immunology, 8, 166–177. https://doi.org/10.1016/j.fsi.2019.01.011
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